A Novel Tandem Zinc Finger Protein in <i>Gossypium hirsutum</i>, GhTZF2, Interacts with GhMORF8 to Regulate Cotton Fiber Cell Development
Arginine-rich Tandem Zinc Finger (RR-TZF) proteins make up a plant-specific superfamily that participates in plant development, while their roles in cotton fiber development remain to be explored. In this study, we identified an RR-TZF protein-coding gene, <i>GhTZF2</i>, containing two C...
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MDPI AG
2023-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/2/519 |
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| author | Yang Li Wei Xi Jianfeng Hao Li Zhang Xingpeng Wen Zhiguo Wu Yuxian Zhu |
| author_facet | Yang Li Wei Xi Jianfeng Hao Li Zhang Xingpeng Wen Zhiguo Wu Yuxian Zhu |
| author_sort | Yang Li |
| collection | DOAJ |
| description | Arginine-rich Tandem Zinc Finger (RR-TZF) proteins make up a plant-specific superfamily that participates in plant development, while their roles in cotton fiber development remain to be explored. In this study, we identified an RR-TZF protein-coding gene, <i>GhTZF2</i>, containing two CCCH domains (C-X<sub>7</sub>-C-X<sub>5</sub>-C-X<sub>3</sub>-H-X<sub>16</sub>-C-X<sub>5</sub>-C-X<sub>4</sub>-C-X<sub>3</sub>-H) and one RR domain at the N-terminus of the two CCCH domains, by comparing the differences of chromatin H3K4me3 modifications between wild-type upland cotton (WT) and the <i>fuzzless-lintless</i> mutant (<i>fl</i>) ovules. <i>GhTZF2</i> was highly expressed in ovule cells near anthesis, and multiple experiments revealed that <i>GhTZF2</i> could interact directly with GhMORF8. Homozygotic <i>GhTZF2</i>-knockout cotton lines produced significantly shorter fibers with thinner cell walls. Additionally, comparative transcriptome analysis confirmed that many differentially expressed transcripts contain adenine- and uridine-rich (AU-rich) elements (AREs) in their 3’ untranslated regions (UTR). Together, this study indicated that <i>GhTZF2</i> may regulate cotton fiber cell development through interacting with GhMORF8, or may be involved in mRNA turnover. |
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| last_indexed | 2024-03-11T09:17:12Z |
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| series | Agronomy |
| spelling | doaj.art-a54f3a05e7e54a14b61a793f91ea6fc52023-11-16T18:35:48ZengMDPI AGAgronomy2073-43952023-02-0113251910.3390/agronomy13020519A Novel Tandem Zinc Finger Protein in <i>Gossypium hirsutum</i>, GhTZF2, Interacts with GhMORF8 to Regulate Cotton Fiber Cell DevelopmentYang Li0Wei Xi1Jianfeng Hao2Li Zhang3Xingpeng Wen4Zhiguo Wu5Yuxian Zhu6State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaArginine-rich Tandem Zinc Finger (RR-TZF) proteins make up a plant-specific superfamily that participates in plant development, while their roles in cotton fiber development remain to be explored. In this study, we identified an RR-TZF protein-coding gene, <i>GhTZF2</i>, containing two CCCH domains (C-X<sub>7</sub>-C-X<sub>5</sub>-C-X<sub>3</sub>-H-X<sub>16</sub>-C-X<sub>5</sub>-C-X<sub>4</sub>-C-X<sub>3</sub>-H) and one RR domain at the N-terminus of the two CCCH domains, by comparing the differences of chromatin H3K4me3 modifications between wild-type upland cotton (WT) and the <i>fuzzless-lintless</i> mutant (<i>fl</i>) ovules. <i>GhTZF2</i> was highly expressed in ovule cells near anthesis, and multiple experiments revealed that <i>GhTZF2</i> could interact directly with GhMORF8. Homozygotic <i>GhTZF2</i>-knockout cotton lines produced significantly shorter fibers with thinner cell walls. Additionally, comparative transcriptome analysis confirmed that many differentially expressed transcripts contain adenine- and uridine-rich (AU-rich) elements (AREs) in their 3’ untranslated regions (UTR). Together, this study indicated that <i>GhTZF2</i> may regulate cotton fiber cell development through interacting with GhMORF8, or may be involved in mRNA turnover.https://www.mdpi.com/2073-4395/13/2/519fiber development<i>GhMORF8</i><i>GhTZF2</i>RNA turnoverRR-TZF |
| spellingShingle | Yang Li Wei Xi Jianfeng Hao Li Zhang Xingpeng Wen Zhiguo Wu Yuxian Zhu A Novel Tandem Zinc Finger Protein in <i>Gossypium hirsutum</i>, GhTZF2, Interacts with GhMORF8 to Regulate Cotton Fiber Cell Development Agronomy fiber development <i>GhMORF8</i> <i>GhTZF2</i> RNA turnover RR-TZF |
| title | A Novel Tandem Zinc Finger Protein in <i>Gossypium hirsutum</i>, GhTZF2, Interacts with GhMORF8 to Regulate Cotton Fiber Cell Development |
| title_full | A Novel Tandem Zinc Finger Protein in <i>Gossypium hirsutum</i>, GhTZF2, Interacts with GhMORF8 to Regulate Cotton Fiber Cell Development |
| title_fullStr | A Novel Tandem Zinc Finger Protein in <i>Gossypium hirsutum</i>, GhTZF2, Interacts with GhMORF8 to Regulate Cotton Fiber Cell Development |
| title_full_unstemmed | A Novel Tandem Zinc Finger Protein in <i>Gossypium hirsutum</i>, GhTZF2, Interacts with GhMORF8 to Regulate Cotton Fiber Cell Development |
| title_short | A Novel Tandem Zinc Finger Protein in <i>Gossypium hirsutum</i>, GhTZF2, Interacts with GhMORF8 to Regulate Cotton Fiber Cell Development |
| title_sort | novel tandem zinc finger protein in i gossypium hirsutum i ghtzf2 interacts with ghmorf8 to regulate cotton fiber cell development |
| topic | fiber development <i>GhMORF8</i> <i>GhTZF2</i> RNA turnover RR-TZF |
| url | https://www.mdpi.com/2073-4395/13/2/519 |
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