Effect of Traditionally Used Neem and Babool Chewing Stick (Datun) on Streptococcus Mutans: An In–Vitro Study
Purpose: There are various plants, which are used as chewing sticks in different parts of the world. Several studies have been reported on the antimicrobial effects of chewing sticks on oral bacteria. This study was conducted to evaluate the effectiveness of traditionally used neem and babool ch...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/4549/9817_CE[Ra1]_F(DK0_PF1(PAK)_PFA(AK)_PH2(AGAK)_PF2(PN).pdf |
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| author | ABHISHEK SHARMA BHARAT SANKHLA SUJAL M PARKAR SUDHEER HONGAL THANVEER K AJITHKRISHNAN CG |
| author_facet | ABHISHEK SHARMA BHARAT SANKHLA SUJAL M PARKAR SUDHEER HONGAL THANVEER K AJITHKRISHNAN CG |
| author_sort | ABHISHEK SHARMA |
| collection | DOAJ |
| description | Purpose: There are various plants, which are used as chewing
sticks in different parts of the world. Several studies have been
reported on the antimicrobial effects of chewing sticks on oral
bacteria. This study was conducted to evaluate the effectiveness
of traditionally used neem and babool chewing sticks (datun)
extracts on Streptococcus mutans.
Materials and Methods: The present invitro study was conducted
to assess effectiveness of 5%, 10%, and 50% neem and babool
extract on Streptococcus mutans. The ditch plate method was
used to test the antimicrobial activity. Ditches were prepared on
blood agar plates with the help of punch having 6mm diameter.
The plates were left for 1h at room temperature and then incubated
at 37°C for 48h and examined for zone of inhibition.
Results: There was no zone of inhibition observed with 5% babool
and neem aqueous extract. There was significant difference in
mean diameter of zone of inhibition of 10% neem and babool
extract (p-value 0.001 < 0.05). Similarly the mean difference
in 50% neem and babool extract was found to be significant
(p-value < 0.001).
Conclusion: Both neem and babool extracts had antimicrobial
activity against Streptococcus mutans, while antimicrobial activity
was significantly higher in neem aqueous extract than babool
aqueous extract. |
| first_indexed | 2024-12-13T06:16:05Z |
| format | Article |
| id | doaj.art-a54f55f8291d41f188f5e8912c3834dd |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-13T06:16:05Z |
| publishDate | 2014-07-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-a54f55f8291d41f188f5e8912c3834dd2022-12-21T23:56:58ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-07-0187ZC15ZC1710.7860/JCDR/2014/9817.4549Effect of Traditionally Used Neem and Babool Chewing Stick (Datun) on Streptococcus Mutans: An In–Vitro StudyABHISHEK SHARMA0BHARAT SANKHLA1SUJAL M PARKAR2SUDHEER HONGAL3THANVEER K4AJITHKRISHNAN CG5Assistant Professor, Department of Public Health Dentistry, Government Dental College & Hospital, Jaipur, India.Assistant Professor, Department of Oral Pathology, Government Dental College & Hospital, Jaipur, India.Assistant Professor, Department of Public Health Dentistry, Siddhpur Dental College, Patan, Gujarat, India.Reader, Department of Public Health Dentistry, Peoples Dental Academy, Bhopal, India.Professsor, Department of Public Health Dentistry, K M Shah Dental College & Hospital, Gujarat, India.Professsor, Department of Public Health Dentistry, K M Shah Dental College & Hospital, Gujarat, India.Purpose: There are various plants, which are used as chewing sticks in different parts of the world. Several studies have been reported on the antimicrobial effects of chewing sticks on oral bacteria. This study was conducted to evaluate the effectiveness of traditionally used neem and babool chewing sticks (datun) extracts on Streptococcus mutans. Materials and Methods: The present invitro study was conducted to assess effectiveness of 5%, 10%, and 50% neem and babool extract on Streptococcus mutans. The ditch plate method was used to test the antimicrobial activity. Ditches were prepared on blood agar plates with the help of punch having 6mm diameter. The plates were left for 1h at room temperature and then incubated at 37°C for 48h and examined for zone of inhibition. Results: There was no zone of inhibition observed with 5% babool and neem aqueous extract. There was significant difference in mean diameter of zone of inhibition of 10% neem and babool extract (p-value 0.001 < 0.05). Similarly the mean difference in 50% neem and babool extract was found to be significant (p-value < 0.001). Conclusion: Both neem and babool extracts had antimicrobial activity against Streptococcus mutans, while antimicrobial activity was significantly higher in neem aqueous extract than babool aqueous extract.https://jcdr.net/articles/PDF/4549/9817_CE[Ra1]_F(DK0_PF1(PAK)_PFA(AK)_PH2(AGAK)_PF2(PN).pdfantimicrobial activitychewing sticksoral bacteriazone of inhibition |
| spellingShingle | ABHISHEK SHARMA BHARAT SANKHLA SUJAL M PARKAR SUDHEER HONGAL THANVEER K AJITHKRISHNAN CG Effect of Traditionally Used Neem and Babool Chewing Stick (Datun) on Streptococcus Mutans: An In–Vitro Study Journal of Clinical and Diagnostic Research antimicrobial activity chewing sticks oral bacteria zone of inhibition |
| title | Effect of Traditionally Used Neem and Babool Chewing Stick (Datun) on Streptococcus Mutans: An In–Vitro Study |
| title_full | Effect of Traditionally Used Neem and Babool Chewing Stick (Datun) on Streptococcus Mutans: An In–Vitro Study |
| title_fullStr | Effect of Traditionally Used Neem and Babool Chewing Stick (Datun) on Streptococcus Mutans: An In–Vitro Study |
| title_full_unstemmed | Effect of Traditionally Used Neem and Babool Chewing Stick (Datun) on Streptococcus Mutans: An In–Vitro Study |
| title_short | Effect of Traditionally Used Neem and Babool Chewing Stick (Datun) on Streptococcus Mutans: An In–Vitro Study |
| title_sort | effect of traditionally used neem and babool chewing stick datun on streptococcus mutans an in vitro study |
| topic | antimicrobial activity chewing sticks oral bacteria zone of inhibition |
| url | https://jcdr.net/articles/PDF/4549/9817_CE[Ra1]_F(DK0_PF1(PAK)_PFA(AK)_PH2(AGAK)_PF2(PN).pdf |
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