The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study
Danshensu, a traditional herb-based active component (<i>Salvia miltiorrhiza</i> Bunge), has garnered attention, due to its safety, nutritional value, and antioxidant effects, along with cardiovascular-protective and neuroprotective abilities; however, its effect on the retinal tissues a...
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MDPI AG
2021-03-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/3/978 |
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| author | Yun-Wen Chen Yun-Ping Huang Pei-Chang Wu Wei-Yu Chiang Ping-Hsun Wang Bo-Yie Chen |
| author_facet | Yun-Wen Chen Yun-Ping Huang Pei-Chang Wu Wei-Yu Chiang Ping-Hsun Wang Bo-Yie Chen |
| author_sort | Yun-Wen Chen |
| collection | DOAJ |
| description | Danshensu, a traditional herb-based active component (<i>Salvia miltiorrhiza</i> Bunge), has garnered attention, due to its safety, nutritional value, and antioxidant effects, along with cardiovascular-protective and neuroprotective abilities; however, its effect on the retinal tissues and functional vision has not been fully studied. The objective of this study was to analyze the protective effect of danshensu on retinal tissues and functional vision in vivo in a mouse model of light-induced retinal degeneration. High energy light-evoked visual damage was confirmed by the loss in structural tissue integrity in the retina accompanied by a decline in visual acuity and visual contrast sensitivity function (VCSF), whereas the retina tissue exhibited severe Müller cell gliosis. Although danshensu treatment did not particularly reduce light-evoked damage to the photoreceptors, it significantly prevented Müller cell gliosis. Danshensu exerted protective effects against light-evoked deterioration on low spatial frequency-based VCSF as determined by the behavioral optomotor reflex method. Additionally, the protective effect of danshensu on VCSF can be reversed and blocked by the injection of a dopamine D1 receptor antagonist (SCH 23390). This study demonstrated that the major functional vision promotional effect of danshensu in vivo was through the dopamine D1 receptors enhancement pathway, rather than the structural protection of the retinas. |
| first_indexed | 2024-03-10T13:08:52Z |
| format | Article |
| id | doaj.art-a551cd5f3917477db126b013acfbc8be |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T13:08:52Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-a551cd5f3917477db126b013acfbc8be2023-11-21T10:56:34ZengMDPI AGNutrients2072-66432021-03-0113397810.3390/nu13030978The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo StudyYun-Wen Chen0Yun-Ping Huang1Pei-Chang Wu2Wei-Yu Chiang3Ping-Hsun Wang4Bo-Yie Chen5Department of Ophthalmology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 88301, TaiwanDepartment of Optometry, Chung Shan Medical University, Taichung 40201, TaiwanDepartment of Ophthalmology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 88301, TaiwanDepartment of Ophthalmology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 88301, TaiwanDepartment of Optometry, Chung Shan Medical University, Taichung 40201, TaiwanDepartment of Optometry, Chung Shan Medical University, Taichung 40201, TaiwanDanshensu, a traditional herb-based active component (<i>Salvia miltiorrhiza</i> Bunge), has garnered attention, due to its safety, nutritional value, and antioxidant effects, along with cardiovascular-protective and neuroprotective abilities; however, its effect on the retinal tissues and functional vision has not been fully studied. The objective of this study was to analyze the protective effect of danshensu on retinal tissues and functional vision in vivo in a mouse model of light-induced retinal degeneration. High energy light-evoked visual damage was confirmed by the loss in structural tissue integrity in the retina accompanied by a decline in visual acuity and visual contrast sensitivity function (VCSF), whereas the retina tissue exhibited severe Müller cell gliosis. Although danshensu treatment did not particularly reduce light-evoked damage to the photoreceptors, it significantly prevented Müller cell gliosis. Danshensu exerted protective effects against light-evoked deterioration on low spatial frequency-based VCSF as determined by the behavioral optomotor reflex method. Additionally, the protective effect of danshensu on VCSF can be reversed and blocked by the injection of a dopamine D1 receptor antagonist (SCH 23390). This study demonstrated that the major functional vision promotional effect of danshensu in vivo was through the dopamine D1 receptors enhancement pathway, rather than the structural protection of the retinas.https://www.mdpi.com/2072-6643/13/3/978danshensuretinafunctional visionvisual contrast sensitivity function |
| spellingShingle | Yun-Wen Chen Yun-Ping Huang Pei-Chang Wu Wei-Yu Chiang Ping-Hsun Wang Bo-Yie Chen The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study Nutrients danshensu retina functional vision visual contrast sensitivity function |
| title | The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study |
| title_full | The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study |
| title_fullStr | The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study |
| title_full_unstemmed | The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study |
| title_short | The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study |
| title_sort | functional vision protection effect of danshensu via dopamine d1 receptors in vivo study |
| topic | danshensu retina functional vision visual contrast sensitivity function |
| url | https://www.mdpi.com/2072-6643/13/3/978 |
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