The Porcine Nasal Microbiota with Particular Attention to Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> in Germany—A Culturomic Approach
Livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) remains a serious public health threat. Porcine nasal cavities are predominant habitats of LA-MRSA. Hence, components of their microbiota might be of interest as putative antagonistically acting competitors...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/4/514 |
| _version_ | 1797571477601517568 |
|---|---|
| author | Andreas Schlattmann Knut von Lützau Ursula Kaspar Karsten Becker |
| author_facet | Andreas Schlattmann Knut von Lützau Ursula Kaspar Karsten Becker |
| author_sort | Andreas Schlattmann |
| collection | DOAJ |
| description | Livestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) remains a serious public health threat. Porcine nasal cavities are predominant habitats of LA-MRSA. Hence, components of their microbiota might be of interest as putative antagonistically acting competitors. Here, an extensive culturomics approach has been applied including 27 healthy pigs from seven different farms; five were treated with antibiotics prior to sampling. Overall, 314 different species with standing in nomenclature and 51 isolates representing novel bacterial taxa were detected. <i>Staphylococcus aureus</i> was isolated from pigs on all seven farms sampled, comprising ten different <i>spa</i> types with t899 (<i>n</i> = 15, 29.4%) and t337 (<i>n</i> = 10, 19.6%) being most frequently isolated. Twenty-six MRSA (mostly t899) were detected on five out of the seven farms. Positive correlations between MRSA colonization and age and colonization with <i>Streptococcus hyovaginalis</i>, and a negative correlation between colonization with MRSA and <i>Citrobacter</i> spp. were found (<i>p</i> < 0.05). Of 209 non-<i>S. aureus</i> members of the <i>Staphylococcaceae</i> family, 25 isolates (12.0%) from three out of the seven farms exhibited methicillin resistance, including two <i>Macrococcus goetzii</i> isolates carrying the <i>mecB</i> gene. Among 125 <i>Enterobacterales</i>, none tested positive for extended-spectrum beta-lactamase (ESBL) and carbapenemase production. The high frequency of methicillin-resistant staphylococci supports the need for enhanced efforts within the “One Health” concept to manage the antibiotic resistance crisis in the human and veterinary medicine sector. |
| first_indexed | 2024-03-10T20:41:13Z |
| format | Article |
| id | doaj.art-a5547c14db2a4a7ea0c66d1d24409afc |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:41:13Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a5547c14db2a4a7ea0c66d1d24409afc2023-11-19T20:42:10ZengMDPI AGMicroorganisms2076-26072020-04-018451410.3390/microorganisms8040514The Porcine Nasal Microbiota with Particular Attention to Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> in Germany—A Culturomic ApproachAndreas Schlattmann0Knut von Lützau1Ursula Kaspar2Karsten Becker3Institute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyLivestock-associated methicillin-resistant <i>Staphylococcus aureus</i> (LA-MRSA) remains a serious public health threat. Porcine nasal cavities are predominant habitats of LA-MRSA. Hence, components of their microbiota might be of interest as putative antagonistically acting competitors. Here, an extensive culturomics approach has been applied including 27 healthy pigs from seven different farms; five were treated with antibiotics prior to sampling. Overall, 314 different species with standing in nomenclature and 51 isolates representing novel bacterial taxa were detected. <i>Staphylococcus aureus</i> was isolated from pigs on all seven farms sampled, comprising ten different <i>spa</i> types with t899 (<i>n</i> = 15, 29.4%) and t337 (<i>n</i> = 10, 19.6%) being most frequently isolated. Twenty-six MRSA (mostly t899) were detected on five out of the seven farms. Positive correlations between MRSA colonization and age and colonization with <i>Streptococcus hyovaginalis</i>, and a negative correlation between colonization with MRSA and <i>Citrobacter</i> spp. were found (<i>p</i> < 0.05). Of 209 non-<i>S. aureus</i> members of the <i>Staphylococcaceae</i> family, 25 isolates (12.0%) from three out of the seven farms exhibited methicillin resistance, including two <i>Macrococcus goetzii</i> isolates carrying the <i>mecB</i> gene. Among 125 <i>Enterobacterales</i>, none tested positive for extended-spectrum beta-lactamase (ESBL) and carbapenemase production. The high frequency of methicillin-resistant staphylococci supports the need for enhanced efforts within the “One Health” concept to manage the antibiotic resistance crisis in the human and veterinary medicine sector.https://www.mdpi.com/2076-2607/8/4/514StaphylococcusMRSAmicrobiotapigMacrococcusESBL |
| spellingShingle | Andreas Schlattmann Knut von Lützau Ursula Kaspar Karsten Becker The Porcine Nasal Microbiota with Particular Attention to Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> in Germany—A Culturomic Approach Microorganisms Staphylococcus MRSA microbiota pig Macrococcus ESBL |
| title | The Porcine Nasal Microbiota with Particular Attention to Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> in Germany—A Culturomic Approach |
| title_full | The Porcine Nasal Microbiota with Particular Attention to Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> in Germany—A Culturomic Approach |
| title_fullStr | The Porcine Nasal Microbiota with Particular Attention to Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> in Germany—A Culturomic Approach |
| title_full_unstemmed | The Porcine Nasal Microbiota with Particular Attention to Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> in Germany—A Culturomic Approach |
| title_short | The Porcine Nasal Microbiota with Particular Attention to Livestock-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> in Germany—A Culturomic Approach |
| title_sort | porcine nasal microbiota with particular attention to livestock associated methicillin resistant i staphylococcus aureus i in germany a culturomic approach |
| topic | Staphylococcus MRSA microbiota pig Macrococcus ESBL |
| url | https://www.mdpi.com/2076-2607/8/4/514 |
| work_keys_str_mv | AT andreasschlattmann theporcinenasalmicrobiotawithparticularattentiontolivestockassociatedmethicillinresistantistaphylococcusaureusiingermanyaculturomicapproach AT knutvonlutzau theporcinenasalmicrobiotawithparticularattentiontolivestockassociatedmethicillinresistantistaphylococcusaureusiingermanyaculturomicapproach AT ursulakaspar theporcinenasalmicrobiotawithparticularattentiontolivestockassociatedmethicillinresistantistaphylococcusaureusiingermanyaculturomicapproach AT karstenbecker theporcinenasalmicrobiotawithparticularattentiontolivestockassociatedmethicillinresistantistaphylococcusaureusiingermanyaculturomicapproach AT andreasschlattmann porcinenasalmicrobiotawithparticularattentiontolivestockassociatedmethicillinresistantistaphylococcusaureusiingermanyaculturomicapproach AT knutvonlutzau porcinenasalmicrobiotawithparticularattentiontolivestockassociatedmethicillinresistantistaphylococcusaureusiingermanyaculturomicapproach AT ursulakaspar porcinenasalmicrobiotawithparticularattentiontolivestockassociatedmethicillinresistantistaphylococcusaureusiingermanyaculturomicapproach AT karstenbecker porcinenasalmicrobiotawithparticularattentiontolivestockassociatedmethicillinresistantistaphylococcusaureusiingermanyaculturomicapproach |