Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec
Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of bloodstream infections (BSIs). We aimed to study molecular epidemiological characteristics of MRSA isolates from BSIs in northern Japan to elucidate the recent trend of their clonal diversity. Methods: MRS...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-03-01
|
| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520303222 |
| _version_ | 1819004971904925696 |
|---|---|
| author | Meiji Soe Aung Noriko Urushibara Mitsuyo Kawaguchiya Mina Hirose Masahiko Ito Satoshi Habadera Nobumichi Kobayashi |
| author_facet | Meiji Soe Aung Noriko Urushibara Mitsuyo Kawaguchiya Mina Hirose Masahiko Ito Satoshi Habadera Nobumichi Kobayashi |
| author_sort | Meiji Soe Aung |
| collection | DOAJ |
| description | Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of bloodstream infections (BSIs). We aimed to study molecular epidemiological characteristics of MRSA isolates from BSIs in northern Japan to elucidate the recent trend of their clonal diversity. Methods: MRSA isolates (n = 277) were collected from blood samples of patients who attended healthcare facilities in Hokkaido, the northern main island of Japan, for a two-year period from August 2017. Genotypes, virulence factors/drug-resistance determinants, and structure of SCCmec complex were analysed by PCR and sequencing analysis. Results: SCCmec-IIa (n = 171, 61.7%) with coagulase genotype (coa-) II, ST5/ST764/ST2389 was the most common genetic trait, followed by SCCmec-IVa (n = 78, 28.2%), and IVl (n = 10, 3.6%). Among the MRSA-IVa, 14 isolates (5.1% of all the isolates) had genetic features identical to USA300 clone (ST8/coa-IIIa/spa-t008 having ΦSa2USA and ACME-I), while PVL/ACME-negative MRSA-IVa isolates (n = 64) were classified into coa-IIa/IIIa/VIIa/VIIb, with coa-VIIa/spa-t1784/ST1 being dominant. Other minor clones included ST8-SCCmec-I, and ST30/ST45/ST81/ST121/ST1232-SCCmec-V, among which the ST1232 isolate harboured PVL genes. Spermidine N-acetyltransferase gene (speG), which is typically present in ACME-I of USA300 clone, was also identified in two isolates, ACME-II’-positive ST764-MRSA-IIa and ACME-negative ST1-MRSA-IVa, showing resistance to spermine. speG of these isolates was located in additional SCCs adjacent to SCCmec. Conclusions: Our present study revealed clonal diversity of MRSA from BSIs in Japan, with increased prevalence of ST8-USA300. Distinct types of speG-carrying SCCs associated with SCCmec-II or IV were identified. |
| first_indexed | 2024-12-20T23:45:23Z |
| format | Article |
| id | doaj.art-a5558a114d4c438cae038f1d9866928a |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-20T23:45:23Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-a5558a114d4c438cae038f1d9866928a2022-12-21T19:22:58ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-03-0124207214Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmecMeiji Soe Aung0Noriko Urushibara1Mitsuyo Kawaguchiya2Mina Hirose3Masahiko Ito4Satoshi Habadera5Nobumichi Kobayashi6Department of Hygiene, Sapporo Medical University School of Medicine, Hokkaido, Sapporo, Japan; Corresponding author at: Department of Hygiene, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo 060-8556, Japan.Department of Hygiene, Sapporo Medical University School of Medicine, Hokkaido, Sapporo, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Hokkaido, Sapporo, JapanDivision of Pediatric Dentistry, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, JapanSapporo Clinical Laboratory, Hokkaido, Sapporo, JapanSapporo Clinical Laboratory, Hokkaido, Sapporo, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Hokkaido, Sapporo, JapanObjectives: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of bloodstream infections (BSIs). We aimed to study molecular epidemiological characteristics of MRSA isolates from BSIs in northern Japan to elucidate the recent trend of their clonal diversity. Methods: MRSA isolates (n = 277) were collected from blood samples of patients who attended healthcare facilities in Hokkaido, the northern main island of Japan, for a two-year period from August 2017. Genotypes, virulence factors/drug-resistance determinants, and structure of SCCmec complex were analysed by PCR and sequencing analysis. Results: SCCmec-IIa (n = 171, 61.7%) with coagulase genotype (coa-) II, ST5/ST764/ST2389 was the most common genetic trait, followed by SCCmec-IVa (n = 78, 28.2%), and IVl (n = 10, 3.6%). Among the MRSA-IVa, 14 isolates (5.1% of all the isolates) had genetic features identical to USA300 clone (ST8/coa-IIIa/spa-t008 having ΦSa2USA and ACME-I), while PVL/ACME-negative MRSA-IVa isolates (n = 64) were classified into coa-IIa/IIIa/VIIa/VIIb, with coa-VIIa/spa-t1784/ST1 being dominant. Other minor clones included ST8-SCCmec-I, and ST30/ST45/ST81/ST121/ST1232-SCCmec-V, among which the ST1232 isolate harboured PVL genes. Spermidine N-acetyltransferase gene (speG), which is typically present in ACME-I of USA300 clone, was also identified in two isolates, ACME-II’-positive ST764-MRSA-IIa and ACME-negative ST1-MRSA-IVa, showing resistance to spermine. speG of these isolates was located in additional SCCs adjacent to SCCmec. Conclusions: Our present study revealed clonal diversity of MRSA from BSIs in Japan, with increased prevalence of ST8-USA300. Distinct types of speG-carrying SCCs associated with SCCmec-II or IV were identified.http://www.sciencedirect.com/science/article/pii/S2213716520303222Bloodstream infectionMRSAClonal diversityACMEspeGJapan |
| spellingShingle | Meiji Soe Aung Noriko Urushibara Mitsuyo Kawaguchiya Mina Hirose Masahiko Ito Satoshi Habadera Nobumichi Kobayashi Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec Journal of Global Antimicrobial Resistance Bloodstream infection MRSA Clonal diversity ACME speG Japan |
| title | Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec |
| title_full | Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec |
| title_fullStr | Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec |
| title_full_unstemmed | Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec |
| title_short | Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec |
| title_sort | clonal diversity of methicillin resistant staphylococcus aureus mrsa from bloodstream infections in northern japan identification of spermidine n acetyltransferase gene speg in staphylococcal cassette chromosomes sccs associated with type ii and iv sccmec |
| topic | Bloodstream infection MRSA Clonal diversity ACME speG Japan |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520303222 |
| work_keys_str_mv | AT meijisoeaung clonaldiversityofmethicillinresistantstaphylococcusaureusmrsafrombloodstreaminfectionsinnorthernjapanidentificationofspermidinenacetyltransferasegenespeginstaphylococcalcassettechromosomessccsassociatedwithtypeiiandivsccmec AT norikourushibara clonaldiversityofmethicillinresistantstaphylococcusaureusmrsafrombloodstreaminfectionsinnorthernjapanidentificationofspermidinenacetyltransferasegenespeginstaphylococcalcassettechromosomessccsassociatedwithtypeiiandivsccmec AT mitsuyokawaguchiya clonaldiversityofmethicillinresistantstaphylococcusaureusmrsafrombloodstreaminfectionsinnorthernjapanidentificationofspermidinenacetyltransferasegenespeginstaphylococcalcassettechromosomessccsassociatedwithtypeiiandivsccmec AT minahirose clonaldiversityofmethicillinresistantstaphylococcusaureusmrsafrombloodstreaminfectionsinnorthernjapanidentificationofspermidinenacetyltransferasegenespeginstaphylococcalcassettechromosomessccsassociatedwithtypeiiandivsccmec AT masahikoito clonaldiversityofmethicillinresistantstaphylococcusaureusmrsafrombloodstreaminfectionsinnorthernjapanidentificationofspermidinenacetyltransferasegenespeginstaphylococcalcassettechromosomessccsassociatedwithtypeiiandivsccmec AT satoshihabadera clonaldiversityofmethicillinresistantstaphylococcusaureusmrsafrombloodstreaminfectionsinnorthernjapanidentificationofspermidinenacetyltransferasegenespeginstaphylococcalcassettechromosomessccsassociatedwithtypeiiandivsccmec AT nobumichikobayashi clonaldiversityofmethicillinresistantstaphylococcusaureusmrsafrombloodstreaminfectionsinnorthernjapanidentificationofspermidinenacetyltransferasegenespeginstaphylococcalcassettechromosomessccsassociatedwithtypeiiandivsccmec |