Use of Temporary Immersion Systems as an alternative for the <em>in vitro</em> propagation of the plantain cultivar INIVITPV06-30 (<em>Musa</em> AAB)
Temporary Immersion Systems (TIS) had been used for in vitro multiplication of different cultivars of Musa spp. achieving high coefficients of multiplication. This investigation was carried out to focussed on the multiplication of the plantain `INIVITPV06-30' (Musa AAB) in TIS. Independent and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2012-01-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/150 |
| _version_ | 1818217897126789120 |
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| author | Milagros Basail Pérez Víctor Medero Vega Eneida Otero Gálvez Marlenys Torres Delgado Jorge López Torres Manuel Cabrera Jova Arletys Santos Pino Aymé Rayas Cabrera Maricel Bauta Toledo Yoel Beovidez García |
| author_facet | Milagros Basail Pérez Víctor Medero Vega Eneida Otero Gálvez Marlenys Torres Delgado Jorge López Torres Manuel Cabrera Jova Arletys Santos Pino Aymé Rayas Cabrera Maricel Bauta Toledo Yoel Beovidez García |
| author_sort | Milagros Basail Pérez |
| collection | DOAJ |
| description | Temporary Immersion Systems (TIS) had been used for in vitro multiplication of different cultivars of Musa spp. achieving high coefficients of multiplication. This investigation was carried out to focussed on the multiplication of the plantain `INIVITPV06-30' (Musa AAB) in TIS. Independent and consecutive experiments were realized to determine the effect of immersion period, immersion frequency, culture medium volume, time of culture and number of explants per flask on the multiplication of this cultivar in TIS. The best results were obtained with 10 minutes immersions of every 6 hours as immersion frequency, 40 ml culture medium per explant, 90 explants per culture flask and 18 days of culture. Plantain cultivar `INIVITPV06-30' (Musa AAB) was multiplied in TIS. The multiplication coefficient and the quality of plant material were high allowing the scaling of them in the tissue culture commercial laboratories.
Key words: explants, micropropagation, multiplication coefficient. |
| first_indexed | 2024-12-12T07:15:10Z |
| format | Article |
| id | doaj.art-a5589e88c72a45ef89d691408eb14af7 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-12T07:15:10Z |
| publishDate | 2012-01-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-a5589e88c72a45ef89d691408eb14af72022-12-22T00:33:31ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472012-01-01121126Use of Temporary Immersion Systems as an alternative for the <em>in vitro</em> propagation of the plantain cultivar INIVITPV06-30 (<em>Musa</em> AAB)Milagros Basail PérezVíctor Medero VegaEneida Otero GálvezMarlenys Torres DelgadoJorge López TorresManuel Cabrera JovaArletys Santos PinoAymé Rayas CabreraMaricel Bauta ToledoYoel Beovidez GarcíaTemporary Immersion Systems (TIS) had been used for in vitro multiplication of different cultivars of Musa spp. achieving high coefficients of multiplication. This investigation was carried out to focussed on the multiplication of the plantain `INIVITPV06-30' (Musa AAB) in TIS. Independent and consecutive experiments were realized to determine the effect of immersion period, immersion frequency, culture medium volume, time of culture and number of explants per flask on the multiplication of this cultivar in TIS. The best results were obtained with 10 minutes immersions of every 6 hours as immersion frequency, 40 ml culture medium per explant, 90 explants per culture flask and 18 days of culture. Plantain cultivar `INIVITPV06-30' (Musa AAB) was multiplied in TIS. The multiplication coefficient and the quality of plant material were high allowing the scaling of them in the tissue culture commercial laboratories. Key words: explants, micropropagation, multiplication coefficient.https://revista.ibp.co.cu/index.php/BV/article/view/150 |
| spellingShingle | Milagros Basail Pérez Víctor Medero Vega Eneida Otero Gálvez Marlenys Torres Delgado Jorge López Torres Manuel Cabrera Jova Arletys Santos Pino Aymé Rayas Cabrera Maricel Bauta Toledo Yoel Beovidez García Use of Temporary Immersion Systems as an alternative for the <em>in vitro</em> propagation of the plantain cultivar INIVITPV06-30 (<em>Musa</em> AAB) Biotecnología Vegetal |
| title | Use of Temporary Immersion Systems as an alternative for the <em>in vitro</em> propagation of the plantain cultivar INIVITPV06-30 (<em>Musa</em> AAB) |
| title_full | Use of Temporary Immersion Systems as an alternative for the <em>in vitro</em> propagation of the plantain cultivar INIVITPV06-30 (<em>Musa</em> AAB) |
| title_fullStr | Use of Temporary Immersion Systems as an alternative for the <em>in vitro</em> propagation of the plantain cultivar INIVITPV06-30 (<em>Musa</em> AAB) |
| title_full_unstemmed | Use of Temporary Immersion Systems as an alternative for the <em>in vitro</em> propagation of the plantain cultivar INIVITPV06-30 (<em>Musa</em> AAB) |
| title_short | Use of Temporary Immersion Systems as an alternative for the <em>in vitro</em> propagation of the plantain cultivar INIVITPV06-30 (<em>Musa</em> AAB) |
| title_sort | use of temporary immersion systems as an alternative for the em in vitro em propagation of the plantain cultivar inivitpv06 30 em musa em aab |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/150 |
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