Gut Microbiota across Normal Gestation and Gestational Diabetes Mellitus: A Cohort Analysis
The prevalence of gestational diabetes mellitus (GDM) is a global public health concern. The mechanism that leads to glucose tolerance beyond normal physiological levels to pathogenic conditions remains incompletely understood, and it is speculated that the maternal microbiome may play an important...
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MDPI AG
2022-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/9/796 |
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| author | Patricia M. Dualib Carla R. Taddei Gabriel Fernandes Camila R. S. Carvalho Luiz Gustavo Sparvoli Isis T. Silva Rosiane Mattar Sandra R. G. Ferreira Sergio A. Dib Bianca de Almeida-Pititto |
| author_facet | Patricia M. Dualib Carla R. Taddei Gabriel Fernandes Camila R. S. Carvalho Luiz Gustavo Sparvoli Isis T. Silva Rosiane Mattar Sandra R. G. Ferreira Sergio A. Dib Bianca de Almeida-Pititto |
| author_sort | Patricia M. Dualib |
| collection | DOAJ |
| description | The prevalence of gestational diabetes mellitus (GDM) is a global public health concern. The mechanism that leads to glucose tolerance beyond normal physiological levels to pathogenic conditions remains incompletely understood, and it is speculated that the maternal microbiome may play an important role. This study analyzes the gut microbiota composition in each trimester of weight-matched women with and without GDM and examines possible bacterial genera associations with GDM. This study followed 56 pregnant women with GDM and 59 without admitted to the outpatient clinic during their first/second or third trimester of gestation. They were submitted to a standardized questionnaire, dietary recalls, clinical examination, biological sample collection, and molecular profiling of fecal microbiota. Women with GDM were older and had a higher number of pregnancies than normal-tolerant ones. There was no difference in alpha diversity, and the groups did not differ regarding the overall microbiota structure. A higher abundance of <i>Bacteroides</i> in the GDM group was found. A positive correlation between <i>Christensenellaceae</i> and <i>Intestinobacter</i> abundances with one-hour post-challenge plasma glucose and a negative correlation between <i>Enterococcus</i> and two-hour plasma glucose levels were observed. <i>Bifidobacterium</i> and <i>Peptococcus</i> abundances were increased in the third gestational trimester for both groups. The gut microbiota composition was not dependent on the presence of GDM weight-matched women throughout gestation. However, some genera abundances showed associations with glucose metabolism. Our findings may therefore encourage a deeper understanding of physiological and pathophysiological changes in the microbiota throughout pregnancy, which could have further implications for diseases prevention. |
| first_indexed | 2024-03-09T23:11:59Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T23:11:59Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-a55cfb448ad2428abc43ec9c4a742e322023-11-23T17:43:49ZengMDPI AGMetabolites2218-19892022-08-0112979610.3390/metabo12090796Gut Microbiota across Normal Gestation and Gestational Diabetes Mellitus: A Cohort AnalysisPatricia M. Dualib0Carla R. Taddei1Gabriel Fernandes2Camila R. S. Carvalho3Luiz Gustavo Sparvoli4Isis T. Silva5Rosiane Mattar6Sandra R. G. Ferreira7Sergio A. Dib8Bianca de Almeida-Pititto9Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, Sena Madureira, 1500, Vila Clementino, São Paulo CEP 04021-001, BrazilDepartment of Clinical and Toxicological Analysis and Obstetrics, School of Arts, Sciences and Humanities, Universidade de São Paulo (USP), Av. Prof. Lineu Prestes 580—Bloco 17, São Paulo CEP 05508-000, BrazilDepaBiosystems Informatics and Genomics Group, Instituto René Rachou—Fiocruz Minas, Av. Augusto de Lima, 1714, Belo Horizonte CEP 30190-002, BrazilGraduate Program in Endocrinology and Metabology, Universidade Federal de São Paulo, Rua Estado de Israel, nº 639, Vila Clementino, São Paulo CEP 04022-001, BrazilDepartment of Clinical and Toxicological Analysis and Obstetrics, School of Arts, Sciences and Humanities, Universidade de São Paulo (USP), Av. Prof. Lineu Prestes 580—Bloco 17, São Paulo CEP 05508-000, BrazilNutrition Course, Centro Universitário Estácio de Sá, Rua Erê, 207, Belo Horizonte CEP 30411-052, BrazilDepartament of Obstetrics, Escola Paulista de Medicina, Universidade Federal de São Paulo, R. Napoleão de Barros, 875—Vila Clementino, São Paulo CEP 04024-002, BrazilDepartment of Epidemiology, Escola de Saúde Pública, Universidade de São Paulo, Av. Dr. Arnaldo, 715—Cerqueira César, São Paulo CEP 01246-904, BrazilDepartment of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, Sena Madureira, 1500, Vila Clementino, São Paulo CEP 04021-001, BrazilDepartment of Preventive Medicine, Escola Paulista de Medicina, Campus São Paulo, Universidade Federal de São Paulo, Rua Botucatu, n° 740, Vila Clementino, São Paulo CEP 04023-062, BrazilThe prevalence of gestational diabetes mellitus (GDM) is a global public health concern. The mechanism that leads to glucose tolerance beyond normal physiological levels to pathogenic conditions remains incompletely understood, and it is speculated that the maternal microbiome may play an important role. This study analyzes the gut microbiota composition in each trimester of weight-matched women with and without GDM and examines possible bacterial genera associations with GDM. This study followed 56 pregnant women with GDM and 59 without admitted to the outpatient clinic during their first/second or third trimester of gestation. They were submitted to a standardized questionnaire, dietary recalls, clinical examination, biological sample collection, and molecular profiling of fecal microbiota. Women with GDM were older and had a higher number of pregnancies than normal-tolerant ones. There was no difference in alpha diversity, and the groups did not differ regarding the overall microbiota structure. A higher abundance of <i>Bacteroides</i> in the GDM group was found. A positive correlation between <i>Christensenellaceae</i> and <i>Intestinobacter</i> abundances with one-hour post-challenge plasma glucose and a negative correlation between <i>Enterococcus</i> and two-hour plasma glucose levels were observed. <i>Bifidobacterium</i> and <i>Peptococcus</i> abundances were increased in the third gestational trimester for both groups. The gut microbiota composition was not dependent on the presence of GDM weight-matched women throughout gestation. However, some genera abundances showed associations with glucose metabolism. Our findings may therefore encourage a deeper understanding of physiological and pathophysiological changes in the microbiota throughout pregnancy, which could have further implications for diseases prevention.https://www.mdpi.com/2218-1989/12/9/796gestational diabetes mellitusgut microbiotaobesitypregnancy |
| spellingShingle | Patricia M. Dualib Carla R. Taddei Gabriel Fernandes Camila R. S. Carvalho Luiz Gustavo Sparvoli Isis T. Silva Rosiane Mattar Sandra R. G. Ferreira Sergio A. Dib Bianca de Almeida-Pititto Gut Microbiota across Normal Gestation and Gestational Diabetes Mellitus: A Cohort Analysis Metabolites gestational diabetes mellitus gut microbiota obesity pregnancy |
| title | Gut Microbiota across Normal Gestation and Gestational Diabetes Mellitus: A Cohort Analysis |
| title_full | Gut Microbiota across Normal Gestation and Gestational Diabetes Mellitus: A Cohort Analysis |
| title_fullStr | Gut Microbiota across Normal Gestation and Gestational Diabetes Mellitus: A Cohort Analysis |
| title_full_unstemmed | Gut Microbiota across Normal Gestation and Gestational Diabetes Mellitus: A Cohort Analysis |
| title_short | Gut Microbiota across Normal Gestation and Gestational Diabetes Mellitus: A Cohort Analysis |
| title_sort | gut microbiota across normal gestation and gestational diabetes mellitus a cohort analysis |
| topic | gestational diabetes mellitus gut microbiota obesity pregnancy |
| url | https://www.mdpi.com/2218-1989/12/9/796 |
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