Effects of low nitrogen on seedling growth, photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficiencies
Abstract Nitrogen plays a significant role in influencing various physiological processes in plants, thereby impacting their ability to withstand abiotic stresses. This study used hydroponics to compare the effects of three nitrogen supply levels (1N, 1/2N and 1/4N) on the antioxidant capacity of ri...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-11-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47260-z |
| _version_ | 1797559636078886912 |
|---|---|
| author | Zexin Qi Fenglou Ling Dongsheng Jia Jingjing Cui Zhian Zhang Chen Xu Lintian Yu Chenglong Guan Ye Wang Mengru Zhang Jiaqi Dou |
| author_facet | Zexin Qi Fenglou Ling Dongsheng Jia Jingjing Cui Zhian Zhang Chen Xu Lintian Yu Chenglong Guan Ye Wang Mengru Zhang Jiaqi Dou |
| author_sort | Zexin Qi |
| collection | DOAJ |
| description | Abstract Nitrogen plays a significant role in influencing various physiological processes in plants, thereby impacting their ability to withstand abiotic stresses. This study used hydroponics to compare the effects of three nitrogen supply levels (1N, 1/2N and 1/4N) on the antioxidant capacity of rice varieties JJ88 (nitrogen efficient) and XN999 (nitrogen inefficient) with different nitrogen use efficiencies. The results show that compared with the XN999 variety, the JJ88 variety has stronger adaptability to low-nitrogen conditions, which is mainly reflected in the relatively small decrease in dry weight and net photosynthetic rate (Pn); In the early stage of low-nitrogen treatment (0–7 d), the $${\mathbf{O}}_{2}^{-}$$ O 2 - production rate, hydrogen peroxide (H2O2) and malondialdehyde (MDA) content of JJ88 variety increased relatively slightly, but the superoxide dismutase (SOD), peroxide The activity of enzyme (POD) and catalase (CAT) increased significantly; After low-nitrogen treatment, the ASA–GSH cycle enzyme activity of JJ88 variety was relatively high, and the dehydroascorbate reductase (DHAR) activity after 14 days of low-nitrogen treatment was higher than that of 1N treatment; The content of reduced ascorbic acid (ASA) in non-enzymatic antioxidants was lower than that of 1N treatment after 14 days of low nitrogen treatment; The contents of oxidized dehydroascorbic acid (DHA) and carotenoids (Car) were higher than those of 1N treatment after 21d and 14d of low nitrogen treatment respectively; The contents of reduced glutathione (GSH), oxidized glutathione (GSSG) and proline (Pro) showed a larger upward trend during the entire low-nitrogen treatment period. In summary, the JJ88 rice variety has a strong ability to regulate oxidative stress and osmotic damage under low nitrogen conditions. It can slow down plant damage by regulating antioxidant enzyme activity and antioxidant content. This provides a basis for achieving nitrogen reduction and efficiency improvement in rice and the breeding of nitrogen-efficient varieties. |
| first_indexed | 2024-03-10T17:48:08Z |
| format | Article |
| id | doaj.art-a55d938e6f0f494d922a40b168e0a334 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:48:08Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a55d938e6f0f494d922a40b168e0a3342023-11-20T09:28:25ZengNature PortfolioScientific Reports2045-23222023-11-0113111310.1038/s41598-023-47260-zEffects of low nitrogen on seedling growth, photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficienciesZexin Qi0Fenglou Ling1Dongsheng Jia2Jingjing Cui3Zhian Zhang4Chen Xu5Lintian Yu6Chenglong Guan7Ye Wang8Mengru Zhang9Jiaqi Dou10College of Agriculture, Jilin Agricultural UniversityCollege of Agriculture, Jilin Agricultural UniversityCollege of Agriculture, Jilin Agricultural UniversityCollege of Agriculture, Jilin Agricultural UniversityCollege of Agriculture, Jilin Agricultural UniversityInstitute of Agricultural Resources and Environment Research, Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China)College of Agriculture, Jilin Agricultural UniversityCollege of Agriculture, Jilin Agricultural UniversityCollege of Agriculture, Jilin Agricultural UniversityCollege of Agriculture, Jilin Agricultural UniversityCollege of Agriculture, Jilin Agricultural UniversityAbstract Nitrogen plays a significant role in influencing various physiological processes in plants, thereby impacting their ability to withstand abiotic stresses. This study used hydroponics to compare the effects of three nitrogen supply levels (1N, 1/2N and 1/4N) on the antioxidant capacity of rice varieties JJ88 (nitrogen efficient) and XN999 (nitrogen inefficient) with different nitrogen use efficiencies. The results show that compared with the XN999 variety, the JJ88 variety has stronger adaptability to low-nitrogen conditions, which is mainly reflected in the relatively small decrease in dry weight and net photosynthetic rate (Pn); In the early stage of low-nitrogen treatment (0–7 d), the $${\mathbf{O}}_{2}^{-}$$ O 2 - production rate, hydrogen peroxide (H2O2) and malondialdehyde (MDA) content of JJ88 variety increased relatively slightly, but the superoxide dismutase (SOD), peroxide The activity of enzyme (POD) and catalase (CAT) increased significantly; After low-nitrogen treatment, the ASA–GSH cycle enzyme activity of JJ88 variety was relatively high, and the dehydroascorbate reductase (DHAR) activity after 14 days of low-nitrogen treatment was higher than that of 1N treatment; The content of reduced ascorbic acid (ASA) in non-enzymatic antioxidants was lower than that of 1N treatment after 14 days of low nitrogen treatment; The contents of oxidized dehydroascorbic acid (DHA) and carotenoids (Car) were higher than those of 1N treatment after 21d and 14d of low nitrogen treatment respectively; The contents of reduced glutathione (GSH), oxidized glutathione (GSSG) and proline (Pro) showed a larger upward trend during the entire low-nitrogen treatment period. In summary, the JJ88 rice variety has a strong ability to regulate oxidative stress and osmotic damage under low nitrogen conditions. It can slow down plant damage by regulating antioxidant enzyme activity and antioxidant content. This provides a basis for achieving nitrogen reduction and efficiency improvement in rice and the breeding of nitrogen-efficient varieties.https://doi.org/10.1038/s41598-023-47260-z |
| spellingShingle | Zexin Qi Fenglou Ling Dongsheng Jia Jingjing Cui Zhian Zhang Chen Xu Lintian Yu Chenglong Guan Ye Wang Mengru Zhang Jiaqi Dou Effects of low nitrogen on seedling growth, photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficiencies Scientific Reports |
| title | Effects of low nitrogen on seedling growth, photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficiencies |
| title_full | Effects of low nitrogen on seedling growth, photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficiencies |
| title_fullStr | Effects of low nitrogen on seedling growth, photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficiencies |
| title_full_unstemmed | Effects of low nitrogen on seedling growth, photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficiencies |
| title_short | Effects of low nitrogen on seedling growth, photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficiencies |
| title_sort | effects of low nitrogen on seedling growth photosynthetic characteristics and antioxidant system of rice varieties with different nitrogen efficiencies |
| url | https://doi.org/10.1038/s41598-023-47260-z |
| work_keys_str_mv | AT zexinqi effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT fenglouling effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT dongshengjia effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT jingjingcui effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT zhianzhang effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT chenxu effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT lintianyu effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT chenglongguan effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT yewang effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT mengruzhang effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies AT jiaqidou effectsoflownitrogenonseedlinggrowthphotosyntheticcharacteristicsandantioxidantsystemofricevarietieswithdifferentnitrogenefficiencies |