Formation of intraneuronal iron deposits following local release from nanostructured silica injected into rat brain parenchyma
Nanostructured materials with controllable properties have been used to cage and release various types of compounds. In the present study, iron-loaded nanostructured sol-gel SiO2–Fe materials were prepared and injected into the rat brain to develop a method for gradual iron delivery into the neurons...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024038179 |
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| author | E. Ortiz-Islas A.A. Ponce-Juárez F. Tzompantzi-Morales M.E. Manríquez-Ramírez C. Rubio M. Calvillo-Velasco G. Chávez-Cortes F. Missirlis M. Rubio-Osornio |
| author_facet | E. Ortiz-Islas A.A. Ponce-Juárez F. Tzompantzi-Morales M.E. Manríquez-Ramírez C. Rubio M. Calvillo-Velasco G. Chávez-Cortes F. Missirlis M. Rubio-Osornio |
| author_sort | E. Ortiz-Islas |
| collection | DOAJ |
| description | Nanostructured materials with controllable properties have been used to cage and release various types of compounds. In the present study, iron-loaded nanostructured sol-gel SiO2–Fe materials were prepared and injected into the rat brain to develop a method for gradual iron delivery into the neurons with the aims to avoid acute iron toxicity and develop an animal model of gradual, metal-induced neurodegeneration. Nanoparticles were prepared by the traditional method of hydrolysis and condensation reactions of tetraethyl orthosilicate at room temperature and subsequent heat treatment at 200 °C. FeSO4 was added in situ during the silica preparation. The resulting materials were characterized by UV-VIS and infrared spectroscopies, X-ray diffraction, and N2 adsorption-desorption. An in vitro ferrous sulfate release test was carried out in artificial cerebrospinal fluid as the release medium showing successful ferrous sulfate loading on nanostructured silica and sustained iron release during the test time of 10 h. Male Wistar rats administered with SiO2–Fe nanoparticles in the substantia nigra pars compacta (SNpc) showed significant intraneuronal increase of iron, in contrast to the animals administered with FeSO4 that showed severe neuronal loss, 72 h post-treatment. Both treatments induced lipid fluorescent product formation in the ventral midbrain, in contrast to iron-free SiO2 and PBS-only injection controls. Circling behavior was evaluated six days after the intranigral microinjection, considered as a behavioral end-point of brain damage. The apomorphine-induced ipsilateral turns in the treated animals presented significant differences in relation to the control groups, with FeSO4 administration leading to a dramatic phenotype, compared to a milder impact in SiO2–Fe administrated animals. Thus, the use of SiO2–Fe nanoparticles represents a slow iron release system useful to model the gradual iron-accumulation process observed in the SNpc of patients with idiopathic Parkinson's disease. |
| first_indexed | 2024-04-24T13:49:34Z |
| format | Article |
| id | doaj.art-a565c26790084341abc087aab514ddd8 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:49:34Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-a565c26790084341abc087aab514ddd82024-04-04T05:05:55ZengElsevierHeliyon2405-84402024-03-01106e27786Formation of intraneuronal iron deposits following local release from nanostructured silica injected into rat brain parenchymaE. Ortiz-Islas0A.A. Ponce-Juárez1F. Tzompantzi-Morales2M.E. Manríquez-Ramírez3C. Rubio4M. Calvillo-Velasco5G. Chávez-Cortes6F. Missirlis7M. Rubio-Osornio8Laboratory of Molecular Neuropharmacology and Nanotechnology, National Institute of Neurology and Neurosurgery, Manuel Velasco Suarez. Insurgentes Sur 3877. Col. La Fama, 14269., Mexico City, MexicoDoctoral Program in Biomedical Sciences, National University Autonomous of Mexico. Universidad 3004, Copilco, Coyoacán, 04510, Mexico City, MexicoMetropolitan Autonomous University-Iztapalapa. Av. San Rafael Atlixco, Iztapalapa, 09340., Mexico City, MexicoESIQIE-National Polytechnic Institute. Instituto Politécnico Nacional s/n, Col. Zacatenco, 07738, Mexico City, MexicoNeurophysiology Department, National Institute of Neurology and Neurosurgery, Manuel Velasco Suarez. Insurgentes Sur 3877. Col. La Fama, 14269., Mexico City, MexicoExperimental Laboratory of Neurodegenerative Diseases, National Institute of Neurology and Neurosurgery, Manuel Velasco Suarez. Insurgentes Sur 3877. Col. La Fama, 14269., Mexico City, MexicoExperimental Laboratory of Neurodegenerative Diseases, National Institute of Neurology and Neurosurgery, Manuel Velasco Suarez. Insurgentes Sur 3877. Col. La Fama, 14269., Mexico City, MexicoDepartment of Physiology, Biophysics and Neuroscience, Cinvestav. Avenida Instituto Politécnico Nacional 2508, San Pedro Zacatenco, Gustavo A. Madero, 07360., Mexico City, MexicoNeurochemistry Department, National Institute of Neurology and Neurosurgery, Manuel Velasco Suarez. Insurgentes Sur 3877. Col. La Fama, 14269., Mexico City, Mexico; Corresponding author. Neurochemistry Department National Institute of Neurology and Neurosurgery Ave. Insurgentes Sur No. 3877, Col. La Fama Mexico City P.C., 14269, Mexico.Nanostructured materials with controllable properties have been used to cage and release various types of compounds. In the present study, iron-loaded nanostructured sol-gel SiO2–Fe materials were prepared and injected into the rat brain to develop a method for gradual iron delivery into the neurons with the aims to avoid acute iron toxicity and develop an animal model of gradual, metal-induced neurodegeneration. Nanoparticles were prepared by the traditional method of hydrolysis and condensation reactions of tetraethyl orthosilicate at room temperature and subsequent heat treatment at 200 °C. FeSO4 was added in situ during the silica preparation. The resulting materials were characterized by UV-VIS and infrared spectroscopies, X-ray diffraction, and N2 adsorption-desorption. An in vitro ferrous sulfate release test was carried out in artificial cerebrospinal fluid as the release medium showing successful ferrous sulfate loading on nanostructured silica and sustained iron release during the test time of 10 h. Male Wistar rats administered with SiO2–Fe nanoparticles in the substantia nigra pars compacta (SNpc) showed significant intraneuronal increase of iron, in contrast to the animals administered with FeSO4 that showed severe neuronal loss, 72 h post-treatment. Both treatments induced lipid fluorescent product formation in the ventral midbrain, in contrast to iron-free SiO2 and PBS-only injection controls. Circling behavior was evaluated six days after the intranigral microinjection, considered as a behavioral end-point of brain damage. The apomorphine-induced ipsilateral turns in the treated animals presented significant differences in relation to the control groups, with FeSO4 administration leading to a dramatic phenotype, compared to a milder impact in SiO2–Fe administrated animals. Thus, the use of SiO2–Fe nanoparticles represents a slow iron release system useful to model the gradual iron-accumulation process observed in the SNpc of patients with idiopathic Parkinson's disease.http://www.sciencedirect.com/science/article/pii/S2405844024038179Nanostructured SiO2SiO2–FeIronParkinson's disease |
| spellingShingle | E. Ortiz-Islas A.A. Ponce-Juárez F. Tzompantzi-Morales M.E. Manríquez-Ramírez C. Rubio M. Calvillo-Velasco G. Chávez-Cortes F. Missirlis M. Rubio-Osornio Formation of intraneuronal iron deposits following local release from nanostructured silica injected into rat brain parenchyma Heliyon Nanostructured SiO2 SiO2–Fe Iron Parkinson's disease |
| title | Formation of intraneuronal iron deposits following local release from nanostructured silica injected into rat brain parenchyma |
| title_full | Formation of intraneuronal iron deposits following local release from nanostructured silica injected into rat brain parenchyma |
| title_fullStr | Formation of intraneuronal iron deposits following local release from nanostructured silica injected into rat brain parenchyma |
| title_full_unstemmed | Formation of intraneuronal iron deposits following local release from nanostructured silica injected into rat brain parenchyma |
| title_short | Formation of intraneuronal iron deposits following local release from nanostructured silica injected into rat brain parenchyma |
| title_sort | formation of intraneuronal iron deposits following local release from nanostructured silica injected into rat brain parenchyma |
| topic | Nanostructured SiO2 SiO2–Fe Iron Parkinson's disease |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024038179 |
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