Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients
Abstract Background The respiratory microbiome is altered in COPD patients but its relationship with core components of the disease, such as the severity of airflow limitation, the frequency of exacerbations or the circulating levels of eosinophils, is unclear. Methods Cross-sectional study comprisi...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-06-01
|
| Series: | BMC Pulmonary Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12890-019-0867-x |
| _version_ | 1818201445252464640 |
|---|---|
| author | Laura Millares Sergi Pascual Concepción Montón Marian García-Núñez Cristina Lalmolda Rosa Faner Carme Casadevall Laia Setó Silvia Capilla Amàlia Moreno Ady Angélica Castro-Acosta Carlos José Alvarez-Martinez Oriol Sibila Germán Peces-Barba Borja G. Cosio Alvar Agustí Joaquim Gea Eduard Monsó |
| author_facet | Laura Millares Sergi Pascual Concepción Montón Marian García-Núñez Cristina Lalmolda Rosa Faner Carme Casadevall Laia Setó Silvia Capilla Amàlia Moreno Ady Angélica Castro-Acosta Carlos José Alvarez-Martinez Oriol Sibila Germán Peces-Barba Borja G. Cosio Alvar Agustí Joaquim Gea Eduard Monsó |
| author_sort | Laura Millares |
| collection | DOAJ |
| description | Abstract Background The respiratory microbiome is altered in COPD patients but its relationship with core components of the disease, such as the severity of airflow limitation, the frequency of exacerbations or the circulating levels of eosinophils, is unclear. Methods Cross-sectional study comprising 72 clinically stable COPD patients (mean age 68 [SD 7.9] years; FEV1 48.7 [SD 20.1]% of reference) who provided spontaneous sputum samples for 16S rRNA gene amplification and sequencing. The microbiome composition was analysed with QIIME. Results We observed that: (1) more severe airflow limitation was associated with reduced relative abundance (RA) of Treponema and an increase in Pseudomonas; (2) patients with ≥2 exacerbations the previous year showed a significantly different bacterial community with respect to non-exacerbators (p = 0.014), with changes in 13 genera, including an increase of Pseudomonas, and finally, (3) peripheral eosinophils levels ≥2% were associated with more diverse microbiome [Chao1 224.51 (74.88) vs 277.39 (78.92) p = 0.006; Shannon 3.94 (1.05) vs 4.54 (1.06) p = 0.020], and a significant increase in the RAs of 20 genera. Conclusion The respiratory microbiome in clinically stable COPD patients varies significantly according to the severity of airflow limitation, previous history of exacerbations and circulating eosinophils levels. |
| first_indexed | 2024-12-12T02:53:40Z |
| format | Article |
| id | doaj.art-a568107fbe3c4613b078ece0b4c21c5a |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-12-12T02:53:40Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-a568107fbe3c4613b078ece0b4c21c5a2022-12-22T00:40:49ZengBMCBMC Pulmonary Medicine1471-24662019-06-011911910.1186/s12890-019-0867-xRelationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patientsLaura Millares0Sergi Pascual1Concepción Montón2Marian García-Núñez3Cristina Lalmolda4Rosa Faner5Carme Casadevall6Laia Setó7Silvia Capilla8Amàlia Moreno9Ady Angélica Castro-Acosta10Carlos José Alvarez-Martinez11Oriol Sibila12Germán Peces-Barba13Borja G. Cosio14Alvar Agustí15Joaquim Gea16Eduard Monsó17Fundació Parc Taulí- Institut d’ Investigació i Innovació Parc Taulí (I3PT)Respiratory Medicine Department, Hospital del Mar – IMIMUniversitat Autònoma de Barcelona, Esfera UABFundació Parc Taulí- Institut d’ Investigació i Innovació Parc Taulí (I3PT)Fundació Parc Taulí- Institut d’ Investigació i Innovació Parc Taulí (I3PT)Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII)Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII)Fundació Parc Taulí- Institut d’ Investigació i Innovació Parc Taulí (I3PT)Department of Microbiology, Hospital Universitari Parc TaulíDepartment of Respiratory Medicine, Hospital Universitari Parc TaulíCentro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII)Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII)Barcelona Respiratory NetworkCentro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII)Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII)Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII)Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII)Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III (ISCIII)Abstract Background The respiratory microbiome is altered in COPD patients but its relationship with core components of the disease, such as the severity of airflow limitation, the frequency of exacerbations or the circulating levels of eosinophils, is unclear. Methods Cross-sectional study comprising 72 clinically stable COPD patients (mean age 68 [SD 7.9] years; FEV1 48.7 [SD 20.1]% of reference) who provided spontaneous sputum samples for 16S rRNA gene amplification and sequencing. The microbiome composition was analysed with QIIME. Results We observed that: (1) more severe airflow limitation was associated with reduced relative abundance (RA) of Treponema and an increase in Pseudomonas; (2) patients with ≥2 exacerbations the previous year showed a significantly different bacterial community with respect to non-exacerbators (p = 0.014), with changes in 13 genera, including an increase of Pseudomonas, and finally, (3) peripheral eosinophils levels ≥2% were associated with more diverse microbiome [Chao1 224.51 (74.88) vs 277.39 (78.92) p = 0.006; Shannon 3.94 (1.05) vs 4.54 (1.06) p = 0.020], and a significant increase in the RAs of 20 genera. Conclusion The respiratory microbiome in clinically stable COPD patients varies significantly according to the severity of airflow limitation, previous history of exacerbations and circulating eosinophils levels.http://link.springer.com/article/10.1186/s12890-019-0867-xBacterial communityDiversityEosinophilsExacerbationsSputumStable COPD |
| spellingShingle | Laura Millares Sergi Pascual Concepción Montón Marian García-Núñez Cristina Lalmolda Rosa Faner Carme Casadevall Laia Setó Silvia Capilla Amàlia Moreno Ady Angélica Castro-Acosta Carlos José Alvarez-Martinez Oriol Sibila Germán Peces-Barba Borja G. Cosio Alvar Agustí Joaquim Gea Eduard Monsó Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients BMC Pulmonary Medicine Bacterial community Diversity Eosinophils Exacerbations Sputum Stable COPD |
| title | Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients |
| title_full | Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients |
| title_fullStr | Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients |
| title_full_unstemmed | Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients |
| title_short | Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients |
| title_sort | relationship between the respiratory microbiome and the severity of airflow limitation history of exacerbations and circulating eosinophils in copd patients |
| topic | Bacterial community Diversity Eosinophils Exacerbations Sputum Stable COPD |
| url | http://link.springer.com/article/10.1186/s12890-019-0867-x |
| work_keys_str_mv | AT lauramillares relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT sergipascual relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT concepcionmonton relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT mariangarcianunez relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT cristinalalmolda relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT rosafaner relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT carmecasadevall relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT laiaseto relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT silviacapilla relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT amaliamoreno relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT adyangelicacastroacosta relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT carlosjosealvarezmartinez relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT oriolsibila relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT germanpecesbarba relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT borjagcosio relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT alvaragusti relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT joaquimgea relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients AT eduardmonso relationshipbetweentherespiratorymicrobiomeandtheseverityofairflowlimitationhistoryofexacerbationsandcirculatingeosinophilsincopdpatients |