Isolation and Characterization of the Zearalenone-Degrading Strain, <i>Bacillus spizizenii</i> B73, Inspired by Esterase Activity
Zearalenone (ZEN) is a widespread mycotoxin found in grain and feed, presenting a serious threat to animal and human health. This study investigated the ability of the novel strain B73, isolated from petroleum-contaminated soil, to detoxify ZEN. B73 was identified as <i>Bacillus spizizenii<...
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MDPI AG
2023-08-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/8/488 |
| _version_ | 1827728570108084224 |
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| author | Xue Liu Na Wu Mingyu Zhang Feng Xue Qing Xu |
| author_facet | Xue Liu Na Wu Mingyu Zhang Feng Xue Qing Xu |
| author_sort | Xue Liu |
| collection | DOAJ |
| description | Zearalenone (ZEN) is a widespread mycotoxin found in grain and feed, presenting a serious threat to animal and human health. This study investigated the ability of the novel strain B73, isolated from petroleum-contaminated soil, to detoxify ZEN. B73 was identified as <i>Bacillus spizizenii</i> through physiological and biochemical tests, and further confirmed based on the 16S rRNA gene sequence and the complete genome sequence. <i>B. spizizenii</i> B73 was capable of degrading up to 99.3% of ZEN at a concentration of 10 μg/mL in a minimal medium (pH = 7.0) within 8 h at 37 °C via HPLC-UV. In addition, <i>B. spizizenii</i> B73 was used to treat ZEN-contaminated wheat bran, dried distillers grains (DDGS), and corn meal, whereby the respective degradation rates reached 96.32%, 98.73%, and 80.31% after 36 h of treatment. HPLC-Q-Exactive-MS/MS analysis revealed one of the degradation products to have the formula C<sub>17</sub>H<sub>24</sub>O<sub>4</sub>. <i>B. spizizenii</i> B73 is a novel strain isolated from petroleum-contaminated soil, and the extracellular enzymes secreted by this strain show a remarkable ability to degrade ZEN. |
| first_indexed | 2024-03-10T23:31:53Z |
| format | Article |
| id | doaj.art-a5682ae82a8240068637aac8d144a6ff |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T23:31:53Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-a5682ae82a8240068637aac8d144a6ff2023-11-19T03:15:19ZengMDPI AGToxins2072-66512023-08-0115848810.3390/toxins15080488Isolation and Characterization of the Zearalenone-Degrading Strain, <i>Bacillus spizizenii</i> B73, Inspired by Esterase ActivityXue Liu0Na Wu1Mingyu Zhang2Feng Xue3Qing Xu4School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210046, ChinaCollege of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224007, ChinaSchool of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210046, ChinaSchool of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210046, ChinaSchool of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210046, ChinaZearalenone (ZEN) is a widespread mycotoxin found in grain and feed, presenting a serious threat to animal and human health. This study investigated the ability of the novel strain B73, isolated from petroleum-contaminated soil, to detoxify ZEN. B73 was identified as <i>Bacillus spizizenii</i> through physiological and biochemical tests, and further confirmed based on the 16S rRNA gene sequence and the complete genome sequence. <i>B. spizizenii</i> B73 was capable of degrading up to 99.3% of ZEN at a concentration of 10 μg/mL in a minimal medium (pH = 7.0) within 8 h at 37 °C via HPLC-UV. In addition, <i>B. spizizenii</i> B73 was used to treat ZEN-contaminated wheat bran, dried distillers grains (DDGS), and corn meal, whereby the respective degradation rates reached 96.32%, 98.73%, and 80.31% after 36 h of treatment. HPLC-Q-Exactive-MS/MS analysis revealed one of the degradation products to have the formula C<sub>17</sub>H<sub>24</sub>O<sub>4</sub>. <i>B. spizizenii</i> B73 is a novel strain isolated from petroleum-contaminated soil, and the extracellular enzymes secreted by this strain show a remarkable ability to degrade ZEN.https://www.mdpi.com/2072-6651/15/8/488zearalenone<i>Bacillus spizizenii</i>detoxificationcell extractenzyme |
| spellingShingle | Xue Liu Na Wu Mingyu Zhang Feng Xue Qing Xu Isolation and Characterization of the Zearalenone-Degrading Strain, <i>Bacillus spizizenii</i> B73, Inspired by Esterase Activity Toxins zearalenone <i>Bacillus spizizenii</i> detoxification cell extract enzyme |
| title | Isolation and Characterization of the Zearalenone-Degrading Strain, <i>Bacillus spizizenii</i> B73, Inspired by Esterase Activity |
| title_full | Isolation and Characterization of the Zearalenone-Degrading Strain, <i>Bacillus spizizenii</i> B73, Inspired by Esterase Activity |
| title_fullStr | Isolation and Characterization of the Zearalenone-Degrading Strain, <i>Bacillus spizizenii</i> B73, Inspired by Esterase Activity |
| title_full_unstemmed | Isolation and Characterization of the Zearalenone-Degrading Strain, <i>Bacillus spizizenii</i> B73, Inspired by Esterase Activity |
| title_short | Isolation and Characterization of the Zearalenone-Degrading Strain, <i>Bacillus spizizenii</i> B73, Inspired by Esterase Activity |
| title_sort | isolation and characterization of the zearalenone degrading strain i bacillus spizizenii i b73 inspired by esterase activity |
| topic | zearalenone <i>Bacillus spizizenii</i> detoxification cell extract enzyme |
| url | https://www.mdpi.com/2072-6651/15/8/488 |
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