Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source
Detection of animal species in meat product is crucial to prevent adulterated and unnecessary contamination during processing, in addition to avoid allergy and religious consequences. Gold standard is the real-time PCR assays, which has a limited target capability. In this study, we have established...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/10/1392 |
| _version_ | 1797552018014863360 |
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| author | Jonas Kissenkötter Susanne Böhlken-Fascher Ahmed Abd El Wahed |
| author_facet | Jonas Kissenkötter Susanne Böhlken-Fascher Ahmed Abd El Wahed |
| author_sort | Jonas Kissenkötter |
| collection | DOAJ |
| description | Detection of animal species in meat product is crucial to prevent adulterated and unnecessary contamination during processing, in addition to avoid allergy and religious consequences. Gold standard is the real-time PCR assays, which has a limited target capability. In this study, we have established a rapid sequencing protocol to identify animal species within hours. Sequencing was achieved by nanopore sequencing and data analysis <i>via</i> offline BLAST search. The whole procedure was conducted in a mobile suitcase lab. As per national and international regulations, the developed assay detected adulteration of pork meat with 0.1% of horse, chicken, turkey, cattle, sheep, duck, rabbit, goat, and donkey. The developed test could be used on-site as a rapid and mobile detection system to determine contamination of meat products. |
| first_indexed | 2024-03-10T15:54:09Z |
| format | Article |
| id | doaj.art-a568a768f7db4ac6b707feecc6bf6966 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T15:54:09Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a568a768f7db4ac6b707feecc6bf69662023-11-20T15:49:11ZengMDPI AGFoods2304-81582020-10-01910139210.3390/foods9101392Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat SourceJonas Kissenkötter0Susanne Böhlken-Fascher1Ahmed Abd El Wahed2Department of Animal Science, Division of Microbiology and Animal Hygiene, Georg-August-University of Goettingen, D-37077, Goettingen, GermanyDepartment of Animal Science, Division of Microbiology and Animal Hygiene, Georg-August-University of Goettingen, D-37077, Goettingen, GermanyDepartment of Animal Science, Division of Microbiology and Animal Hygiene, Georg-August-University of Goettingen, D-37077, Goettingen, GermanyDetection of animal species in meat product is crucial to prevent adulterated and unnecessary contamination during processing, in addition to avoid allergy and religious consequences. Gold standard is the real-time PCR assays, which has a limited target capability. In this study, we have established a rapid sequencing protocol to identify animal species within hours. Sequencing was achieved by nanopore sequencing and data analysis <i>via</i> offline BLAST search. The whole procedure was conducted in a mobile suitcase lab. As per national and international regulations, the developed assay detected adulteration of pork meat with 0.1% of horse, chicken, turkey, cattle, sheep, duck, rabbit, goat, and donkey. The developed test could be used on-site as a rapid and mobile detection system to determine contamination of meat products.https://www.mdpi.com/2304-8158/9/10/1392meat adulterationrapid sequencing protocolnanopore sequencingpoint-of-need |
| spellingShingle | Jonas Kissenkötter Susanne Böhlken-Fascher Ahmed Abd El Wahed Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source Foods meat adulteration rapid sequencing protocol nanopore sequencing point-of-need |
| title | Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source |
| title_full | Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source |
| title_fullStr | Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source |
| title_full_unstemmed | Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source |
| title_short | Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source |
| title_sort | flesh id nanopore sequencing combined with offline blast search for the identification of meat source |
| topic | meat adulteration rapid sequencing protocol nanopore sequencing point-of-need |
| url | https://www.mdpi.com/2304-8158/9/10/1392 |
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