Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro
Detection and quantification of senescent cells remain difficult due to variable phenotypes and the absence of highly specific and reliable biomarkers. It is therefore widely accepted to use a combination of multiple markers and cellular characteristics to define senescent cells in vitro. The exact...
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MDPI AG
2022-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/9/1489 |
| _version_ | 1797505272468471808 |
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| author | Tom Zimmermann Michaela Pommer Viola Kluge Chafia Chiheb Susanne Muehlich Anja-Katrin Bosserhoff |
| author_facet | Tom Zimmermann Michaela Pommer Viola Kluge Chafia Chiheb Susanne Muehlich Anja-Katrin Bosserhoff |
| author_sort | Tom Zimmermann |
| collection | DOAJ |
| description | Detection and quantification of senescent cells remain difficult due to variable phenotypes and the absence of highly specific and reliable biomarkers. It is therefore widely accepted to use a combination of multiple markers and cellular characteristics to define senescent cells in vitro. The exact choice of these markers is a subject of ongoing discussion and usually depends on objective reasons such as cell type and treatment conditions, as well as subjective considerations including feasibility and personal experience. This study aims to provide a comprehensive comparison of biomarkers and cellular characteristics used to detect senescence in melanocytic systems. Each marker was assessed in primary human melanocytes that overexpress mutant BRAFV600E, as it is commonly found in melanocytic nevi, and melanoma cells after treatment with the chemotherapeutic agent etoposide. The combined use of these two experimental settings is thought to allow profound conclusions on the choice of senescence biomarkers when working with melanocytic systems. Further, this study supports the development of standardized senescence detection and quantification by providing a comparative analysis that might also be helpful for other cell types and experimental conditions. |
| first_indexed | 2024-03-10T04:16:15Z |
| format | Article |
| id | doaj.art-a56c2b62644142f5833e802e47d65a76 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T04:16:15Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-a56c2b62644142f5833e802e47d65a762023-11-23T07:59:55ZengMDPI AGCells2073-44092022-04-01119148910.3390/cells11091489Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In VitroTom Zimmermann0Michaela Pommer1Viola Kluge2Chafia Chiheb3Susanne Muehlich4Anja-Katrin Bosserhoff5Institute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91052 Erlangen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91052 Erlangen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91052 Erlangen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91052 Erlangen, GermanyDepartment of Chemistry and Pharmacy, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91052 Erlangen, GermanyInstitute of Biochemistry, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91052 Erlangen, GermanyDetection and quantification of senescent cells remain difficult due to variable phenotypes and the absence of highly specific and reliable biomarkers. It is therefore widely accepted to use a combination of multiple markers and cellular characteristics to define senescent cells in vitro. The exact choice of these markers is a subject of ongoing discussion and usually depends on objective reasons such as cell type and treatment conditions, as well as subjective considerations including feasibility and personal experience. This study aims to provide a comprehensive comparison of biomarkers and cellular characteristics used to detect senescence in melanocytic systems. Each marker was assessed in primary human melanocytes that overexpress mutant BRAFV600E, as it is commonly found in melanocytic nevi, and melanoma cells after treatment with the chemotherapeutic agent etoposide. The combined use of these two experimental settings is thought to allow profound conclusions on the choice of senescence biomarkers when working with melanocytic systems. Further, this study supports the development of standardized senescence detection and quantification by providing a comparative analysis that might also be helpful for other cell types and experimental conditions.https://www.mdpi.com/2073-4409/11/9/1489senescencemelanocytemelanomabeta-galactosidase |
| spellingShingle | Tom Zimmermann Michaela Pommer Viola Kluge Chafia Chiheb Susanne Muehlich Anja-Katrin Bosserhoff Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro Cells senescence melanocyte melanoma beta-galactosidase |
| title | Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro |
| title_full | Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro |
| title_fullStr | Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro |
| title_full_unstemmed | Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro |
| title_short | Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro |
| title_sort | detection of cellular senescence in human primary melanocytes and malignant melanoma cells in vitro |
| topic | senescence melanocyte melanoma beta-galactosidase |
| url | https://www.mdpi.com/2073-4409/11/9/1489 |
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