Comparative Analysis of Phytochemical Constituents and Antioxidant Properties of Aqueous and Ethanol Extracts of Alstonia boonei Stem-Bark

The plant Alstonia boonei is extensively used in traditional medicine because of its therapeutic properties. Over the years, researchers have been looking for the active medicinal agents in different plant species and as such, the type of extraction solvent plays a major role. This study aim to inv...

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Main Authors: Mary Chinenye Izum, Stella Oghomwen Olubodun
Format: Article
Language:English
Published: Faculty of Biosciences 2023-10-01
Series:The Bioscientist
Subjects:
Online Access:https://bioscientistjournal.com/index.php/The_Bioscientist/article/view/157
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author Mary Chinenye Izum
Stella Oghomwen Olubodun
author_facet Mary Chinenye Izum
Stella Oghomwen Olubodun
author_sort Mary Chinenye Izum
collection DOAJ
description The plant Alstonia boonei is extensively used in traditional medicine because of its therapeutic properties. Over the years, researchers have been looking for the active medicinal agents in different plant species and as such, the type of extraction solvent plays a major role. This study aim to investigate the comparative analysis of phytochemical constituents and antioxidant properties of aqueous and ethanol extracts of A. boonei stem-bark. The stem-bark of A. boonei was collected, dried, pulverized and extracted with ethanol and distilled water using maceration method. The extract was concentrated in a temperature regulated water bath set at 45oC. Qualitative and quantitative phytochemical analysis was carried out in both extracts. Both extracts was also subjected to various antioxidant analytical procedures such as free radical scavenging capacity against 1, 1-diphenyl-2-picrylhydrazyl (DPPH), reducing power, and ferric reducing antioxidant power (FRAP) to determine antioxidant properties. The qualitative phytochemical analysis of both extracts revealed the presence of flavonoids, cardiac glycoside, terpenoids, alkaloids and saponins. However, quantitative studies revealed that proanthocyanidin, flavonoids and total phenol were significantly higher in ethanol extract compared to aqueous extract (p < 0.05). Both extracts exhibited antioxidant activities compared to reference antioxidant (Ascorbic acid). However, the reducing power of the aqueous extract was significantly higher than that of the ethanol extract.This result indicates that aqueous extract of A. booneistem-bark had lower phytochemical constituents but higher antioxidant activity compared to ethanol extract. The result of this study revealed that ethanol may be a better solvent for phytochemical extraction of A. boonei stem-bark.
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spelling doaj.art-a56fbeb2c498496f960abab18b61dff02023-10-04T12:23:08ZengFaculty of BiosciencesThe Bioscientist2630-71032630-71112023-10-0111310.54117/the_bioscientist.v11i3.157Comparative Analysis of Phytochemical Constituents and Antioxidant Properties of Aqueous and Ethanol Extracts of Alstonia boonei Stem-BarkMary Chinenye IzumStella Oghomwen Olubodun The plant Alstonia boonei is extensively used in traditional medicine because of its therapeutic properties. Over the years, researchers have been looking for the active medicinal agents in different plant species and as such, the type of extraction solvent plays a major role. This study aim to investigate the comparative analysis of phytochemical constituents and antioxidant properties of aqueous and ethanol extracts of A. boonei stem-bark. The stem-bark of A. boonei was collected, dried, pulverized and extracted with ethanol and distilled water using maceration method. The extract was concentrated in a temperature regulated water bath set at 45oC. Qualitative and quantitative phytochemical analysis was carried out in both extracts. Both extracts was also subjected to various antioxidant analytical procedures such as free radical scavenging capacity against 1, 1-diphenyl-2-picrylhydrazyl (DPPH), reducing power, and ferric reducing antioxidant power (FRAP) to determine antioxidant properties. The qualitative phytochemical analysis of both extracts revealed the presence of flavonoids, cardiac glycoside, terpenoids, alkaloids and saponins. However, quantitative studies revealed that proanthocyanidin, flavonoids and total phenol were significantly higher in ethanol extract compared to aqueous extract (p < 0.05). Both extracts exhibited antioxidant activities compared to reference antioxidant (Ascorbic acid). However, the reducing power of the aqueous extract was significantly higher than that of the ethanol extract.This result indicates that aqueous extract of A. booneistem-bark had lower phytochemical constituents but higher antioxidant activity compared to ethanol extract. The result of this study revealed that ethanol may be a better solvent for phytochemical extraction of A. boonei stem-bark. https://bioscientistjournal.com/index.php/The_Bioscientist/article/view/157Alstonia boonei,phytochemical analysisin vitroantioxidant properties
spellingShingle Mary Chinenye Izum
Stella Oghomwen Olubodun
Comparative Analysis of Phytochemical Constituents and Antioxidant Properties of Aqueous and Ethanol Extracts of Alstonia boonei Stem-Bark
The Bioscientist
Alstonia boonei,
phytochemical analysis
in vitro
antioxidant properties
title Comparative Analysis of Phytochemical Constituents and Antioxidant Properties of Aqueous and Ethanol Extracts of Alstonia boonei Stem-Bark
title_full Comparative Analysis of Phytochemical Constituents and Antioxidant Properties of Aqueous and Ethanol Extracts of Alstonia boonei Stem-Bark
title_fullStr Comparative Analysis of Phytochemical Constituents and Antioxidant Properties of Aqueous and Ethanol Extracts of Alstonia boonei Stem-Bark
title_full_unstemmed Comparative Analysis of Phytochemical Constituents and Antioxidant Properties of Aqueous and Ethanol Extracts of Alstonia boonei Stem-Bark
title_short Comparative Analysis of Phytochemical Constituents and Antioxidant Properties of Aqueous and Ethanol Extracts of Alstonia boonei Stem-Bark
title_sort comparative analysis of phytochemical constituents and antioxidant properties of aqueous and ethanol extracts of alstonia boonei stem bark
topic Alstonia boonei,
phytochemical analysis
in vitro
antioxidant properties
url https://bioscientistjournal.com/index.php/The_Bioscientist/article/view/157
work_keys_str_mv AT marychinenyeizum comparativeanalysisofphytochemicalconstituentsandantioxidantpropertiesofaqueousandethanolextractsofalstoniabooneistembark
AT stellaoghomwenolubodun comparativeanalysisofphytochemicalconstituentsandantioxidantpropertiesofaqueousandethanolextractsofalstoniabooneistembark