The RT-PCR additional value in detecting periodontal pathogens in periodontitis patients
Objectives. The aim of this study was to evaluate the prevalence of selected bacterial species from periodontal pockets samples in a group of young adult periodontitis patients and explore the additional value of the Real-Time Polymerase Chain Reaction (RT-PCR) assay. Material and Methods. Patients...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Amaltea Medical Publishing House
2023-06-01
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| Series: | Romanian Journal of Stomatology |
| Subjects: | |
| Online Access: | https://rjs.com.ro/articles/2023.2/RJS_2023_2_Art-03.pdf |
| _version_ | 1797309288636481536 |
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| author | Andrada Soanca Alexandra Roman Iulia Cristina Micu Andrei Picos Laurentiu Catalin Pascu Alina Stanomir Andreea Ciurea |
| author_facet | Andrada Soanca Alexandra Roman Iulia Cristina Micu Andrei Picos Laurentiu Catalin Pascu Alina Stanomir Andreea Ciurea |
| author_sort | Andrada Soanca |
| collection | DOAJ |
| description | Objectives. The aim of this study was to evaluate the prevalence of selected bacterial species from periodontal pockets samples in a group of young adult periodontitis patients and explore the additional value of the Real-Time
Polymerase Chain Reaction (RT-PCR) assay. Material and Methods. Patients with periodontitis diagnosis and good general health were included in the study
after a complete periodontal examination and following the inclusion/exclusion criteria. Samples from the deepest periodontal pockets were collected, and RT-PCR assay was performed in a private laboratory in order to identify eleven bacterial strains associated with periodontitis. The patient’s report provided the bacterial pathogen load quantified according to the detection threshold and classified as negative, weakly positive, positive, or intensely
positive. Results. The RT-PCR assay detected periodontal pathogens, and their prevalence could be calculated based on the test report. The red complex bacteria were detected in all patients with the highest counts. Alongside the orange complex bacteria were also detected in periodontitis patients. A. actinomycetemcomitans remained undetected in our study. Conclusions. The RT-PCR technique proved to be a useful and easy approach for identifying and quantifying subgingival periodontal pathogens in periodontitis patients. RT-PCR testing may facilitate accurate diagnosis and prognosis as well as successful treatment. |
| first_indexed | 2024-03-08T01:23:11Z |
| format | Article |
| id | doaj.art-a5711711a1af40f9b7c0ddad1ba36417 |
| institution | Directory Open Access Journal |
| issn | 1843-0805 2069-6078 |
| language | English |
| last_indexed | 2024-03-08T01:23:11Z |
| publishDate | 2023-06-01 |
| publisher | Amaltea Medical Publishing House |
| record_format | Article |
| series | Romanian Journal of Stomatology |
| spelling | doaj.art-a5711711a1af40f9b7c0ddad1ba364172024-02-14T14:15:57ZengAmaltea Medical Publishing HouseRomanian Journal of Stomatology1843-08052069-60782023-06-01692869310.37897/RJS.2023.2.3The RT-PCR additional value in detecting periodontal pathogens in periodontitis patientsAndrada Soanca0Alexandra Roman1Iulia Cristina Micu2Andrei Picos3Laurentiu Catalin Pascu4Alina Stanomir5Andreea Ciurea6Department of Periodontology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Periodontology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Periodontology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Prevention in Dental Medicine, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Prosthodontics, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Cluj-Napoca, RomaniaDepartment of Periodontology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Department of Periodontology, Faculty of Dental Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania Objectives. The aim of this study was to evaluate the prevalence of selected bacterial species from periodontal pockets samples in a group of young adult periodontitis patients and explore the additional value of the Real-Time Polymerase Chain Reaction (RT-PCR) assay. Material and Methods. Patients with periodontitis diagnosis and good general health were included in the study after a complete periodontal examination and following the inclusion/exclusion criteria. Samples from the deepest periodontal pockets were collected, and RT-PCR assay was performed in a private laboratory in order to identify eleven bacterial strains associated with periodontitis. The patient’s report provided the bacterial pathogen load quantified according to the detection threshold and classified as negative, weakly positive, positive, or intensely positive. Results. The RT-PCR assay detected periodontal pathogens, and their prevalence could be calculated based on the test report. The red complex bacteria were detected in all patients with the highest counts. Alongside the orange complex bacteria were also detected in periodontitis patients. A. actinomycetemcomitans remained undetected in our study. Conclusions. The RT-PCR technique proved to be a useful and easy approach for identifying and quantifying subgingival periodontal pathogens in periodontitis patients. RT-PCR testing may facilitate accurate diagnosis and prognosis as well as successful treatment.https://rjs.com.ro/articles/2023.2/RJS_2023_2_Art-03.pdfperiodontitisrt-pcr assayperiodontal pathogensdiagnostic |
| spellingShingle | Andrada Soanca Alexandra Roman Iulia Cristina Micu Andrei Picos Laurentiu Catalin Pascu Alina Stanomir Andreea Ciurea The RT-PCR additional value in detecting periodontal pathogens in periodontitis patients Romanian Journal of Stomatology periodontitis rt-pcr assay periodontal pathogens diagnostic |
| title | The RT-PCR additional value in detecting periodontal pathogens in periodontitis patients |
| title_full | The RT-PCR additional value in detecting periodontal pathogens in periodontitis patients |
| title_fullStr | The RT-PCR additional value in detecting periodontal pathogens in periodontitis patients |
| title_full_unstemmed | The RT-PCR additional value in detecting periodontal pathogens in periodontitis patients |
| title_short | The RT-PCR additional value in detecting periodontal pathogens in periodontitis patients |
| title_sort | rt pcr additional value in detecting periodontal pathogens in periodontitis patients |
| topic | periodontitis rt-pcr assay periodontal pathogens diagnostic |
| url | https://rjs.com.ro/articles/2023.2/RJS_2023_2_Art-03.pdf |
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