Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young mice
Intermittent fasting as a dietary intervention can prevent overweight and obesity in adult organisms. Nevertheless, information regarding consequences of intermittent fasting for redox status and reactive metabolite-mediated processes that are crucial for the normal functioning of organisms is limit...
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| Format: | Article |
| Language: | English |
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IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
2022-01-01
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| Series: | EXCLI Journal : Experimental and Clinical Sciences |
| Subjects: | |
| Online Access: | https://www.excli.de/index.php/excli/article/view/4347 |
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| author | Oksana V. Kuzniak Oksana M. Sorochynska Maria M. Bayliak Andrii Ya. Klonovskyi Yulia V. Vasylyk Halyna M. Semchyshyn Kenneth B. Storey Olga Garaschuk Volodymyr I. Lushchak |
| author_facet | Oksana V. Kuzniak Oksana M. Sorochynska Maria M. Bayliak Andrii Ya. Klonovskyi Yulia V. Vasylyk Halyna M. Semchyshyn Kenneth B. Storey Olga Garaschuk Volodymyr I. Lushchak |
| author_sort | Oksana V. Kuzniak |
| collection | DOAJ |
| description | Intermittent fasting as a dietary intervention can prevent overweight and obesity in adult organisms. Nevertheless, information regarding consequences of intermittent fasting for redox status and reactive metabolite-mediated processes that are crucial for the normal functioning of organisms is limited. Since the information on effects of intermittent fasting on parameters of oxidative/carbonyl stress in the brains of young mice was absent, the present study addressed these questions using an every-other-day fasting (EODF) protocol. The levels of carbonyl proteins were ~28 %, 22 % and 18 % lower in the cerebral cortex of EODF males and females and middle parts of the brain of EODF males, respectively, as compared to their ad libitum fed counterparts. Lipid peroxides and α-dicarbonyl compounds were lower only in the cortex and medulla part of EODF male brain. The EODF regimen resulted in higher total non-specific antioxidant capacity in different parts of male brain and a tendency to be higher this parameter in females. At the same time, EODF regimen had no effect on the activities of the defensive antioxidant enzymes, namely superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, glyoxylase 1 and glucose-6-phosphate dehydrogenase in the cortex of both sexes, but even decreased activities of these enzymes in medulla and middle part of the brain. In general, the results suggest that in the brain of young mice ad libitum feeding induces mild oxidative/carbonyl stress which may be partially alleviated by the EODF regimen. The effect of EODF regimen is more pronounced in the medulla part than in the cortex. |
| first_indexed | 2024-04-09T17:06:41Z |
| format | Article |
| id | doaj.art-a572add86c9f4cfdba31bf54d746c2ba |
| institution | Directory Open Access Journal |
| issn | 1611-2156 |
| language | English |
| last_indexed | 2024-04-09T17:06:41Z |
| publishDate | 2022-01-01 |
| publisher | IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund |
| record_format | Article |
| series | EXCLI Journal : Experimental and Clinical Sciences |
| spelling | doaj.art-a572add86c9f4cfdba31bf54d746c2ba2023-04-20T13:35:19ZengIfADo - Leibniz Research Centre for Working Environment and Human Factors, DortmundEXCLI Journal : Experimental and Clinical Sciences1611-21562022-01-0121779210.17179/excli2021-43473786Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young miceOksana V. Kuzniak0https://orcid.org/0000-0002-2392-9619Oksana M. Sorochynska1https://orcid.org/0000-0001-5773-760XMaria M. Bayliak2https://orcid.org/0000-0001-6268-8910Andrii Ya. Klonovskyi3https://orcid.org/0000-0001-5719-6751Yulia V. Vasylyk4https://orcid.org/0000-0003-0614-2870Halyna M. Semchyshyn5https://orcid.org/0000-0002-5967-2165Kenneth B. Storey6https://orcid.org/0000-0002-7363-1853Olga Garaschuk7https://orcid.org/0000-0001-7400-5654Volodymyr I. Lushchak8https://orcid.org/0000-0001-5602-3330Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, UkraineDepartment of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, UkraineDepartment of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, UkraineDepartment of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, UkraineDepartment of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, UkraineDepartment of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, UkraineInstitute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, K1S 5B6, CanadaDepartment of Neurophysiology, Institute of Physiology, University of Tübingen, 72074 Tübingen, GermanyDepartment of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine. E-mail: lushchak@pnu.edu.uaIntermittent fasting as a dietary intervention can prevent overweight and obesity in adult organisms. Nevertheless, information regarding consequences of intermittent fasting for redox status and reactive metabolite-mediated processes that are crucial for the normal functioning of organisms is limited. Since the information on effects of intermittent fasting on parameters of oxidative/carbonyl stress in the brains of young mice was absent, the present study addressed these questions using an every-other-day fasting (EODF) protocol. The levels of carbonyl proteins were ~28 %, 22 % and 18 % lower in the cerebral cortex of EODF males and females and middle parts of the brain of EODF males, respectively, as compared to their ad libitum fed counterparts. Lipid peroxides and α-dicarbonyl compounds were lower only in the cortex and medulla part of EODF male brain. The EODF regimen resulted in higher total non-specific antioxidant capacity in different parts of male brain and a tendency to be higher this parameter in females. At the same time, EODF regimen had no effect on the activities of the defensive antioxidant enzymes, namely superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, glyoxylase 1 and glucose-6-phosphate dehydrogenase in the cortex of both sexes, but even decreased activities of these enzymes in medulla and middle part of the brain. In general, the results suggest that in the brain of young mice ad libitum feeding induces mild oxidative/carbonyl stress which may be partially alleviated by the EODF regimen. The effect of EODF regimen is more pronounced in the medulla part than in the cortex.https://www.excli.de/index.php/excli/article/view/4347defensive enzymescarbonyl proteinsα-dicarbonyl compoundsevery-other-day fastinglipid peroxides |
| spellingShingle | Oksana V. Kuzniak Oksana M. Sorochynska Maria M. Bayliak Andrii Ya. Klonovskyi Yulia V. Vasylyk Halyna M. Semchyshyn Kenneth B. Storey Olga Garaschuk Volodymyr I. Lushchak Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young mice EXCLI Journal : Experimental and Clinical Sciences defensive enzymes carbonyl proteins α-dicarbonyl compounds every-other-day fasting lipid peroxides |
| title | Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young mice |
| title_full | Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young mice |
| title_fullStr | Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young mice |
| title_full_unstemmed | Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young mice |
| title_short | Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young mice |
| title_sort | feeding to satiation induces mild oxidative carbonyl stress in the brain of young mice |
| topic | defensive enzymes carbonyl proteins α-dicarbonyl compounds every-other-day fasting lipid peroxides |
| url | https://www.excli.de/index.php/excli/article/view/4347 |
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