Growth hormone gene polymorphism in Jaisalmeri and Sindhi camels
The present study was aimed to assess the genetic polymorphism of growth hormone (GH) gene in Jaisalmeri and Sindhi camels using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique. Genomic DNA were isolated from venous blood samples collected from 93 Indian cam...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2021-09-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/115924 |
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| author | BASANTI JYOTSANA VED PRAKASH SHALINI SUTHAR RAKESH RANJAN |
| author_facet | BASANTI JYOTSANA VED PRAKASH SHALINI SUTHAR RAKESH RANJAN |
| author_sort | BASANTI JYOTSANA |
| collection | DOAJ |
| description | The present study was aimed to assess the genetic polymorphism of growth hormone (GH) gene in Jaisalmeri and Sindhi camels using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique. Genomic DNA were isolated from venous blood samples collected from 93 Indian camels (Jaisalmeri- 38 and Sindhi-55 camels) and 613-base pair fragment of GH gene was amplified from each genomic DNA sample by polymerase chain reaction. The PCR-RFLP was done using restriction enzyme MspI which revealed three genotypes, viz. CC, CT and TT in both the breeds. Our result indicated that both the breeds were polymorphic in nature. The corresponding genotype frequency in Jaisalmeri and Sindhi breeds were 0.474 and 0.345 for the CC, 0.395 and 0.564 for CT, 0.131 and 0.091 for TT genotypes and the corresponding C allele frequency was 0.671 and 0.627 and T allele frequency was 0.329 and 0.373. The CT genotype was found predominant in the Sindhi breed, whereas CC genotype was predominant in Jaisalmeri breed. The frequency of C allele was higher than the T allele in both of the breeds. These camel populations were in Hardy-Weinberg Equilibrium indicating that the populations meet the HWE assumptions and genetic variations were conserved. It could be concluded that Jaisalmeri and Sindhi camels having different genetic variants of GH gene might be investigated for production a DNA marker for growth and production traits in Indian camels. |
| first_indexed | 2024-04-10T07:51:12Z |
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| id | doaj.art-a5730f3ac1674182a6d0e2c38dbd0479 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:51:12Z |
| publishDate | 2021-09-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-a5730f3ac1674182a6d0e2c38dbd04792023-02-23T10:12:02ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272021-09-0191810.56093/ijans.v91i8.115924Growth hormone gene polymorphism in Jaisalmeri and Sindhi camelsBASANTI JYOTSANA0VED PRAKASH1SHALINI SUTHAR2RAKESH RANJAN3ICAR–National Research Centre on Camel, Bikaner, Rajasthan 334 001 IndiaICAR–National Research Centre on Camel, Bikaner, Rajasthan 334 001 IndiaICAR–National Research Centre on Camel, Bikaner, Rajasthan 334 001 IndiaICAR–National Research Centre on Camel, Bikaner, Rajasthan 334 001 IndiaThe present study was aimed to assess the genetic polymorphism of growth hormone (GH) gene in Jaisalmeri and Sindhi camels using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique. Genomic DNA were isolated from venous blood samples collected from 93 Indian camels (Jaisalmeri- 38 and Sindhi-55 camels) and 613-base pair fragment of GH gene was amplified from each genomic DNA sample by polymerase chain reaction. The PCR-RFLP was done using restriction enzyme MspI which revealed three genotypes, viz. CC, CT and TT in both the breeds. Our result indicated that both the breeds were polymorphic in nature. The corresponding genotype frequency in Jaisalmeri and Sindhi breeds were 0.474 and 0.345 for the CC, 0.395 and 0.564 for CT, 0.131 and 0.091 for TT genotypes and the corresponding C allele frequency was 0.671 and 0.627 and T allele frequency was 0.329 and 0.373. The CT genotype was found predominant in the Sindhi breed, whereas CC genotype was predominant in Jaisalmeri breed. The frequency of C allele was higher than the T allele in both of the breeds. These camel populations were in Hardy-Weinberg Equilibrium indicating that the populations meet the HWE assumptions and genetic variations were conserved. It could be concluded that Jaisalmeri and Sindhi camels having different genetic variants of GH gene might be investigated for production a DNA marker for growth and production traits in Indian camels.https://epubs.icar.org.in/index.php/IJAnS/article/view/115924CamelGeneGrowth hormoneJaisalmeriPCR-RFLPPolymorphism |
| spellingShingle | BASANTI JYOTSANA VED PRAKASH SHALINI SUTHAR RAKESH RANJAN Growth hormone gene polymorphism in Jaisalmeri and Sindhi camels Indian Journal of Animal Sciences Camel Gene Growth hormone Jaisalmeri PCR-RFLP Polymorphism |
| title | Growth hormone gene polymorphism in Jaisalmeri and Sindhi camels |
| title_full | Growth hormone gene polymorphism in Jaisalmeri and Sindhi camels |
| title_fullStr | Growth hormone gene polymorphism in Jaisalmeri and Sindhi camels |
| title_full_unstemmed | Growth hormone gene polymorphism in Jaisalmeri and Sindhi camels |
| title_short | Growth hormone gene polymorphism in Jaisalmeri and Sindhi camels |
| title_sort | growth hormone gene polymorphism in jaisalmeri and sindhi camels |
| topic | Camel Gene Growth hormone Jaisalmeri PCR-RFLP Polymorphism |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/115924 |
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