Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province
ObjectiveAntibiotic resistance genes （ARGs） have received wide attention all over the world. The purpose of this study was to explore the bacterial community structure， the types and levels of antibiotic resistance genes in a water body in east China， and to compare and analyze the characteristics o...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Shanghai Preventive Medicine Association
2023-12-01
|
| Series: | Shanghai yufang yixue |
| Subjects: | |
| Online Access: | http://www.sjpm.org.cn/cn/article/doi/10.19428/j.cnki.sjpm.2023.23158 |
| _version_ | 1797198731743854592 |
|---|---|
| author | MAO Chuanwu ZHANG Li ZANG Jinxin JIANG Lufang XIONG Chenglong WANG Na JIANG Feng FU Chaowei HU Jingjing JIANG Qingwu |
| author_facet | MAO Chuanwu ZHANG Li ZANG Jinxin JIANG Lufang XIONG Chenglong WANG Na JIANG Feng FU Chaowei HU Jingjing JIANG Qingwu |
| author_sort | MAO Chuanwu |
| collection | DOAJ |
| description | ObjectiveAntibiotic resistance genes （ARGs） have received wide attention all over the world. The purpose of this study was to explore the bacterial community structure， the types and levels of antibiotic resistance genes in a water body in east China， and to compare and analyze the characteristics of microbial species distribution and antibiotic resistance gene distribution in various water environments.MethodsA total of 10 households in Haimen City， Jiangsu Province were selected and their surrounding water environment samples were collected. 21 water samples including river water （4）， Mingou water （9） and well water （8） were collected for metagenomics sequencing， assembled with MetaWRAP， annotated with CARD database， and analyzed with R software.ResultsIn various water bodies， the dominant bacteria phyla was Proteobacteria， the dominant bacteria genera were Deuterostomia， Pseudomonas， Flavobacteriales and Streptomycetaceae. The ARGs annotated were mainly composed of quinolones， aminoglycosides， macrolides and beta-lactams antibiotic resistance genes. The top four relative abundance of resistance genes were macB， RanA， evgS and TxR， The average absolute abundance and expression of resistance genes in well water and Mingou water were higher than those in river water.ConclusionMultiple ARGs are detected to varying degrees in well water， river water， and Mingou water bodies， and the expression of resistance genes in well water and Mingou water bodies is higher than that in river water bodies， possibly due to human production and living activities. |
| first_indexed | 2024-04-24T07:04:31Z |
| format | Article |
| id | doaj.art-a5743c535d694ebb832c8ef6d9e3c3a5 |
| institution | Directory Open Access Journal |
| issn | 1004-9231 |
| language | zho |
| last_indexed | 2024-04-24T07:04:31Z |
| publishDate | 2023-12-01 |
| publisher | Shanghai Preventive Medicine Association |
| record_format | Article |
| series | Shanghai yufang yixue |
| spelling | doaj.art-a5743c535d694ebb832c8ef6d9e3c3a52024-04-22T05:13:30ZzhoShanghai Preventive Medicine AssociationShanghai yufang yixue1004-92312023-12-0135121199120510.19428/j.cnki.sjpm.2023.231581004-9231（2023）12-1199-07Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu ProvinceMAO Chuanwu0ZHANG Li1ZANG Jinxin2JIANG Lufang3XIONG Chenglong4WANG Na5JIANG Feng6FU Chaowei7HU Jingjing8JIANG Qingwu9School of Public Health， Fudan University， Shanghai 200032， ChinaSchool of Public Health， Fudan University， Shanghai 200032， ChinaSchool of Public Health， Fudan University， Shanghai 200032， ChinaSchool of Public Health， Fudan University， Shanghai 200032， ChinaSchool of Public Health， Fudan University， Shanghai 200032， ChinaSchool of Public Health， Fudan University， Shanghai 200032， ChinaSchool of Public Health， Fudan University， Shanghai 200032， ChinaSchool of Public Health， Fudan University， Shanghai 200032， ChinaShanghai Pinnacles Medical Technology Co.， Shanghai 201114， ChinaSchool of Public Health， Fudan University， Shanghai 200032， ChinaObjectiveAntibiotic resistance genes （ARGs） have received wide attention all over the world. The purpose of this study was to explore the bacterial community structure， the types and levels of antibiotic resistance genes in a water body in east China， and to compare and analyze the characteristics of microbial species distribution and antibiotic resistance gene distribution in various water environments.MethodsA total of 10 households in Haimen City， Jiangsu Province were selected and their surrounding water environment samples were collected. 21 water samples including river water （4）， Mingou water （9） and well water （8） were collected for metagenomics sequencing， assembled with MetaWRAP， annotated with CARD database， and analyzed with R software.ResultsIn various water bodies， the dominant bacteria phyla was Proteobacteria， the dominant bacteria genera were Deuterostomia， Pseudomonas， Flavobacteriales and Streptomycetaceae. The ARGs annotated were mainly composed of quinolones， aminoglycosides， macrolides and beta-lactams antibiotic resistance genes. The top four relative abundance of resistance genes were macB， RanA， evgS and TxR， The average absolute abundance and expression of resistance genes in well water and Mingou water were higher than those in river water.ConclusionMultiple ARGs are detected to varying degrees in well water， river water， and Mingou water bodies， and the expression of resistance genes in well water and Mingou water bodies is higher than that in river water bodies， possibly due to human production and living activities.http://www.sjpm.org.cn/cn/article/doi/10.19428/j.cnki.sjpm.2023.23158antibiotic resistance genemetagenomicsmicrobial diversitywater environment |
| spellingShingle | MAO Chuanwu ZHANG Li ZANG Jinxin JIANG Lufang XIONG Chenglong WANG Na JIANG Feng FU Chaowei HU Jingjing JIANG Qingwu Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province Shanghai yufang yixue antibiotic resistance gene metagenomics microbial diversity water environment |
| title | Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province |
| title_full | Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province |
| title_fullStr | Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province |
| title_full_unstemmed | Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province |
| title_short | Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province |
| title_sort | distribution characteristics of antibiotic resistance genes in the domestic water of residents in haimen jiangsu province |
| topic | antibiotic resistance gene metagenomics microbial diversity water environment |
| url | http://www.sjpm.org.cn/cn/article/doi/10.19428/j.cnki.sjpm.2023.23158 |
| work_keys_str_mv | AT maochuanwu distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince AT zhangli distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince AT zangjinxin distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince AT jianglufang distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince AT xiongchenglong distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince AT wangna distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince AT jiangfeng distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince AT fuchaowei distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince AT hujingjing distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince AT jiangqingwu distributioncharacteristicsofantibioticresistancegenesinthedomesticwaterofresidentsinhaimenjiangsuprovince |