Anti-bacterial and anti-inflammatory activities of lactic acid bacteria-bioconversioned indica rice (Oryza sativa L.) extract
Abstract Background In this study, indica rice (Oryza sativa L.) was bioconversioned using Lactobacillus plantarum ATCC14917 and Lactobacillus rhamnosus GG KCTC5033. Results Compared to the control treatment (RE), rice extract fermented with Lactobacillus plantarum ATCC14917 (LP-RE) and Lactobacillu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-06-01
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| Series: | Chemical and Biological Technologies in Agriculture |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40538-022-00311-5 |
| _version_ | 1818116940313395200 |
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| author | Hyuk-Ju Kwon Hyunwoo Ahn Bong Sun Kim Seok-Seong Kang Kwang-Geun Lee |
| author_facet | Hyuk-Ju Kwon Hyunwoo Ahn Bong Sun Kim Seok-Seong Kang Kwang-Geun Lee |
| author_sort | Hyuk-Ju Kwon |
| collection | DOAJ |
| description | Abstract Background In this study, indica rice (Oryza sativa L.) was bioconversioned using Lactobacillus plantarum ATCC14917 and Lactobacillus rhamnosus GG KCTC5033. Results Compared to the control treatment (RE), rice extract fermented with Lactobacillus plantarum ATCC14917 (LP-RE) and Lactobacillus rhamnosus GG KCTC5033 (LR-RE) inhibited the growth of pathogenic bacteria significantly except for E. coli O157:H7 (p < 0.05). The LR-RE treatment had the strongest inhibitory effect on Staphylococcus aureus biofilm formation (10.81 ± 1.98%). Additionally, real-time PCR analyses confirmed that LP-RE inhibited S. aureus-induced IL-8 expression in Caco-2 cells by 60%. HPLC-orbitrap analysis indicated that this treatment increased the levels of key metabolites with therapeutic properties such as acetophenone, 9-oxo-10(E), 12(E)-octadecadienoic acid, nicotinic acid, and indol-3-lactic acid. Conclusions This study demonstrated that the secondary metabolites were linked to antibacterial and anti-inflammatory effects, suggesting that bioconversion is a promising means to produce dietary supplements with therapeutic properties. Graphical Abstract |
| first_indexed | 2024-12-11T04:30:30Z |
| format | Article |
| id | doaj.art-a57504253e2c4466859cca87460ee2f4 |
| institution | Directory Open Access Journal |
| issn | 2196-5641 |
| language | English |
| last_indexed | 2024-12-11T04:30:30Z |
| publishDate | 2022-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj.art-a57504253e2c4466859cca87460ee2f42022-12-22T01:20:52ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412022-06-01911910.1186/s40538-022-00311-5Anti-bacterial and anti-inflammatory activities of lactic acid bacteria-bioconversioned indica rice (Oryza sativa L.) extractHyuk-Ju Kwon0Hyunwoo Ahn1Bong Sun Kim2Seok-Seong Kang3Kwang-Geun Lee4Department of Food Science and Biotechnology, Dongguk University-SeoulDepartment of Food Science and Biotechnology, Dongguk University-SeoulDepartment of Food Science and Biotechnology, Dongguk University-SeoulDepartment of Food Science and Biotechnology, Dongguk University-SeoulDepartment of Food Science and Biotechnology, Dongguk University-SeoulAbstract Background In this study, indica rice (Oryza sativa L.) was bioconversioned using Lactobacillus plantarum ATCC14917 and Lactobacillus rhamnosus GG KCTC5033. Results Compared to the control treatment (RE), rice extract fermented with Lactobacillus plantarum ATCC14917 (LP-RE) and Lactobacillus rhamnosus GG KCTC5033 (LR-RE) inhibited the growth of pathogenic bacteria significantly except for E. coli O157:H7 (p < 0.05). The LR-RE treatment had the strongest inhibitory effect on Staphylococcus aureus biofilm formation (10.81 ± 1.98%). Additionally, real-time PCR analyses confirmed that LP-RE inhibited S. aureus-induced IL-8 expression in Caco-2 cells by 60%. HPLC-orbitrap analysis indicated that this treatment increased the levels of key metabolites with therapeutic properties such as acetophenone, 9-oxo-10(E), 12(E)-octadecadienoic acid, nicotinic acid, and indol-3-lactic acid. Conclusions This study demonstrated that the secondary metabolites were linked to antibacterial and anti-inflammatory effects, suggesting that bioconversion is a promising means to produce dietary supplements with therapeutic properties. Graphical Abstracthttps://doi.org/10.1186/s40538-022-00311-5Indica riceBioconversionLactic acid bacteriaAnti-bacterialAnti-inflammatorySecondary metabolite |
| spellingShingle | Hyuk-Ju Kwon Hyunwoo Ahn Bong Sun Kim Seok-Seong Kang Kwang-Geun Lee Anti-bacterial and anti-inflammatory activities of lactic acid bacteria-bioconversioned indica rice (Oryza sativa L.) extract Chemical and Biological Technologies in Agriculture Indica rice Bioconversion Lactic acid bacteria Anti-bacterial Anti-inflammatory Secondary metabolite |
| title | Anti-bacterial and anti-inflammatory activities of lactic acid bacteria-bioconversioned indica rice (Oryza sativa L.) extract |
| title_full | Anti-bacterial and anti-inflammatory activities of lactic acid bacteria-bioconversioned indica rice (Oryza sativa L.) extract |
| title_fullStr | Anti-bacterial and anti-inflammatory activities of lactic acid bacteria-bioconversioned indica rice (Oryza sativa L.) extract |
| title_full_unstemmed | Anti-bacterial and anti-inflammatory activities of lactic acid bacteria-bioconversioned indica rice (Oryza sativa L.) extract |
| title_short | Anti-bacterial and anti-inflammatory activities of lactic acid bacteria-bioconversioned indica rice (Oryza sativa L.) extract |
| title_sort | anti bacterial and anti inflammatory activities of lactic acid bacteria bioconversioned indica rice oryza sativa l extract |
| topic | Indica rice Bioconversion Lactic acid bacteria Anti-bacterial Anti-inflammatory Secondary metabolite |
| url | https://doi.org/10.1186/s40538-022-00311-5 |
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