Bioconversion of food waste to biocompatible wet-laid fungal films
The fungus Rhizopus delemar was grown on bread waste in a submerged cultivation process and wet-laid into films. Alkali or enzyme treatments were used to isolate the fungal cell wall. A heat treatment was also applied to deactivate biological activity of the fungus. Homogenization of fungal biomass...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-04-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522001551 |
| _version_ | 1818278375578402816 |
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| author | Maximilian Benedikt Maria Köhnlein Tiffany Abitbol Ana Osório Oliveira Mikael S. Magnusson Karin H. Adolfsson Sofie E. Svensson Jorge A. Ferreira Minna Hakkarainen Akram Zamani |
| author_facet | Maximilian Benedikt Maria Köhnlein Tiffany Abitbol Ana Osório Oliveira Mikael S. Magnusson Karin H. Adolfsson Sofie E. Svensson Jorge A. Ferreira Minna Hakkarainen Akram Zamani |
| author_sort | Maximilian Benedikt Maria Köhnlein |
| collection | DOAJ |
| description | The fungus Rhizopus delemar was grown on bread waste in a submerged cultivation process and wet-laid into films. Alkali or enzyme treatments were used to isolate the fungal cell wall. A heat treatment was also applied to deactivate biological activity of the fungus. Homogenization of fungal biomass was done by an iterative ultrafine grinding process. Finally, the biomass was cast into films by a wet-laid process. Ultrafine grinding resulted in densification of the films. Fungal films showed tensile strengths of up to 18.1 MPa, a Young’s modulus of 2.3 GPa and a strain at break of 1.4%. Highest tensile strength was achieved using alkali treatment, with SEM analysis showing a dense and highly organized structure. In contrast, less organized structures were obtained using enzymatic or heat treatments. A cell viability assay and fluorescent staining confirmed the biocompatibility of the films. A promising route for food waste valorization to sustainable fungal wet-laid films was established. |
| first_indexed | 2024-12-12T23:16:26Z |
| format | Article |
| id | doaj.art-a578b327cde04c33ba6a50f57fa643d7 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-12-12T23:16:26Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-a578b327cde04c33ba6a50f57fa643d72022-12-22T00:08:26ZengElsevierMaterials & Design0264-12752022-04-01216110534Bioconversion of food waste to biocompatible wet-laid fungal filmsMaximilian Benedikt Maria Köhnlein0Tiffany Abitbol1Ana Osório Oliveira2Mikael S. Magnusson3Karin H. Adolfsson4Sofie E. Svensson5Jorge A. Ferreira6Minna Hakkarainen7Akram Zamani8Swedish Centre for Resource Recovery, University of Borås, 501 90 Borås, SwedenRISE - Research Institutes of Sweden, Bioeconomy, Materials and Surfaces, 114 28 Stockholm, SwedenDepartment of Physiology and Pharmacology, Karolinska Institute, 171 77 Stockholm, SwedenRISE - Research Institutes of Sweden, Bioeconomy, Papermaking and Packaging, 114 28 Stockholm, SwedenDepartment of Fibre and Polymer Technology, KTH Royal Institute of Technology, 100 44 Stockholm, SwedenSwedish Centre for Resource Recovery, University of Borås, 501 90 Borås, SwedenSwedish Centre for Resource Recovery, University of Borås, 501 90 Borås, SwedenDepartment of Fibre and Polymer Technology, KTH Royal Institute of Technology, 100 44 Stockholm, SwedenSwedish Centre for Resource Recovery, University of Borås, 501 90 Borås, Sweden; Corresponding author.The fungus Rhizopus delemar was grown on bread waste in a submerged cultivation process and wet-laid into films. Alkali or enzyme treatments were used to isolate the fungal cell wall. A heat treatment was also applied to deactivate biological activity of the fungus. Homogenization of fungal biomass was done by an iterative ultrafine grinding process. Finally, the biomass was cast into films by a wet-laid process. Ultrafine grinding resulted in densification of the films. Fungal films showed tensile strengths of up to 18.1 MPa, a Young’s modulus of 2.3 GPa and a strain at break of 1.4%. Highest tensile strength was achieved using alkali treatment, with SEM analysis showing a dense and highly organized structure. In contrast, less organized structures were obtained using enzymatic or heat treatments. A cell viability assay and fluorescent staining confirmed the biocompatibility of the films. A promising route for food waste valorization to sustainable fungal wet-laid films was established.http://www.sciencedirect.com/science/article/pii/S0264127522001551Filamentous fungiZygomycetesWet-laid filmFood wasteBiocompatibleUltrafine grinding |
| spellingShingle | Maximilian Benedikt Maria Köhnlein Tiffany Abitbol Ana Osório Oliveira Mikael S. Magnusson Karin H. Adolfsson Sofie E. Svensson Jorge A. Ferreira Minna Hakkarainen Akram Zamani Bioconversion of food waste to biocompatible wet-laid fungal films Materials & Design Filamentous fungi Zygomycetes Wet-laid film Food waste Biocompatible Ultrafine grinding |
| title | Bioconversion of food waste to biocompatible wet-laid fungal films |
| title_full | Bioconversion of food waste to biocompatible wet-laid fungal films |
| title_fullStr | Bioconversion of food waste to biocompatible wet-laid fungal films |
| title_full_unstemmed | Bioconversion of food waste to biocompatible wet-laid fungal films |
| title_short | Bioconversion of food waste to biocompatible wet-laid fungal films |
| title_sort | bioconversion of food waste to biocompatible wet laid fungal films |
| topic | Filamentous fungi Zygomycetes Wet-laid film Food waste Biocompatible Ultrafine grinding |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522001551 |
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