Methods to separate nuclear soluble fractions reflecting localizations in living cells

Summary: To understand various intranuclear functions, it is important to know when, what, and how proteins enter the nucleus. Although many methods and commercial kits for nuclear fractionation have been developed, there are still no methods for obtaining a complete nuclear proteome. Soluble nuclea...

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Bibliographic Details
Main Authors: Yutaka Ogawa, Naoko Imamoto
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:iScience
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004221014747