Leaf Protein Electrophoresis and Taxonomy of Species of Jatropha L. (Euphorbiaceae)
The systematic relationship existing among members of the all important genus Jatropha was studied using leaf protein electrophoresis. The aim was to identify possible taxonomic importance of the protein profile in the estimation and elucidation of the taxonomic affinity of the six species of Jatrop...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2012-08-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://notulaebiologicae.ro/index.php/nsb/article/view/7601 |
| _version_ | 1811343037005037568 |
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| author | Olaniran Temitope OLADIPO Herbert Chukwuma ILLOH |
| author_facet | Olaniran Temitope OLADIPO Herbert Chukwuma ILLOH |
| author_sort | Olaniran Temitope OLADIPO |
| collection | DOAJ |
| description | The systematic relationship existing among members of the all important genus Jatropha was studied using leaf protein electrophoresis. The aim was to identify possible taxonomic importance of the protein profile in the estimation and elucidation of the taxonomic affinity of the six species of Jatropha (Jatropha curcas Linn., J. podagrica Hook., J. gossypifolia Linn., J. mutifida Linn., J. tanjorensis Ellis & Saroja and J. integerrima Linn.) found in Nigeria. The species were screened for total protein banding patterns using gel electrophoresis. Young leaves (0.8 g) of the plants were washed with distilled water and macerated with sterile mortar and pestle in 0.8% Phosphate Buffer-Saline (PBS) containing 0.4 M NaCl at pH 8.0. Results reveal that protein banding pattern was taxon specific. Generic band occurs at 8.3. The highest number of interspecific bands (4) exists between J. podagrica and J. multifida. Variations exist not only in the number of bands but also in the intensity of the bands. Sokal and Sneath coefficient of similarity ranges between 11.1-44.4 %. Single linkage Cluster Analysis (SLCA) of the relative mobility values of the protein in the taxa shows partial agreement with current sub generic and sectional delimitation of the species based on morphology and anatomy of the species. |
| first_indexed | 2024-04-13T19:22:39Z |
| format | Article |
| id | doaj.art-a57f6e68e5bb4bc199d7273c643de1dc |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-13T19:22:39Z |
| publishDate | 2012-08-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-a57f6e68e5bb4bc199d7273c643de1dc2022-12-22T02:33:29ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642012-08-014392967274Leaf Protein Electrophoresis and Taxonomy of Species of Jatropha L. (Euphorbiaceae)Olaniran Temitope OLADIPO0Herbert Chukwuma ILLOH1Obafemi Awolowo University, Ile-Ife, Department of BotanyObafemi Awolowo University, Ile-Ife, Department of BotanyThe systematic relationship existing among members of the all important genus Jatropha was studied using leaf protein electrophoresis. The aim was to identify possible taxonomic importance of the protein profile in the estimation and elucidation of the taxonomic affinity of the six species of Jatropha (Jatropha curcas Linn., J. podagrica Hook., J. gossypifolia Linn., J. mutifida Linn., J. tanjorensis Ellis & Saroja and J. integerrima Linn.) found in Nigeria. The species were screened for total protein banding patterns using gel electrophoresis. Young leaves (0.8 g) of the plants were washed with distilled water and macerated with sterile mortar and pestle in 0.8% Phosphate Buffer-Saline (PBS) containing 0.4 M NaCl at pH 8.0. Results reveal that protein banding pattern was taxon specific. Generic band occurs at 8.3. The highest number of interspecific bands (4) exists between J. podagrica and J. multifida. Variations exist not only in the number of bands but also in the intensity of the bands. Sokal and Sneath coefficient of similarity ranges between 11.1-44.4 %. Single linkage Cluster Analysis (SLCA) of the relative mobility values of the protein in the taxa shows partial agreement with current sub generic and sectional delimitation of the species based on morphology and anatomy of the species.http://notulaebiologicae.ro/index.php/nsb/article/view/7601 |
| spellingShingle | Olaniran Temitope OLADIPO Herbert Chukwuma ILLOH Leaf Protein Electrophoresis and Taxonomy of Species of Jatropha L. (Euphorbiaceae) Notulae Scientia Biologicae |
| title | Leaf Protein Electrophoresis and Taxonomy of Species of Jatropha L. (Euphorbiaceae) |
| title_full | Leaf Protein Electrophoresis and Taxonomy of Species of Jatropha L. (Euphorbiaceae) |
| title_fullStr | Leaf Protein Electrophoresis and Taxonomy of Species of Jatropha L. (Euphorbiaceae) |
| title_full_unstemmed | Leaf Protein Electrophoresis and Taxonomy of Species of Jatropha L. (Euphorbiaceae) |
| title_short | Leaf Protein Electrophoresis and Taxonomy of Species of Jatropha L. (Euphorbiaceae) |
| title_sort | leaf protein electrophoresis and taxonomy of species of jatropha l euphorbiaceae |
| url | http://notulaebiologicae.ro/index.php/nsb/article/view/7601 |
| work_keys_str_mv | AT olanirantemitopeoladipo leafproteinelectrophoresisandtaxonomyofspeciesofjatrophaleuphorbiaceae AT herbertchukwumailloh leafproteinelectrophoresisandtaxonomyofspeciesofjatrophaleuphorbiaceae |