GPI-80 Augments NF-κB Activation in Tumor Cells
Recent studies have discovered a relationship between glycosylphosphatidylinositol (GPI)-anchored protein 80 (GPI-80)/VNN2 (80 kDa GPI-anchored protein) and malignant tumors. GPI-80 is known to regulate neutrophil adhesion; however, the action of GPI-80 on tumors is still obscure. In this study, alt...
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| Language: | English |
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/12027 |
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| author | Yuji Takeda Yuta Kurota Tomoyuki Kato Hiromi Ito Akemi Araki Hidetoshi Nara Shinichi Saitoh Nobuyuki Tanaka Norihiko Tsuchiya Hironobu Asao |
| author_facet | Yuji Takeda Yuta Kurota Tomoyuki Kato Hiromi Ito Akemi Araki Hidetoshi Nara Shinichi Saitoh Nobuyuki Tanaka Norihiko Tsuchiya Hironobu Asao |
| author_sort | Yuji Takeda |
| collection | DOAJ |
| description | Recent studies have discovered a relationship between glycosylphosphatidylinositol (GPI)-anchored protein 80 (GPI-80)/VNN2 (80 kDa GPI-anchored protein) and malignant tumors. GPI-80 is known to regulate neutrophil adhesion; however, the action of GPI-80 on tumors is still obscure. In this study, although the expression of GPI-80 mRNA was detectable in several tumor cell lines, the levels of GPI-80 protein were significantly lower than that in neutrophils. To clarify the function of GPI-80 in tumor cells, GPI-80-expressing cells and GPI-80/VNN2 gene-deleted cells were established using PC3 prostate cancer cells. In GPI-80-expressing cells, GPI-80 was mainly detected in vesicles. Furthermore, soluble GPI-80 in the conditioned medium was associated with the exosome marker CD63 and was also detected in the plasma obtained from prostate cancer patients. Unexpectedly, cell adhesion and migration of GPI-80-expressing PC3 cells were not modulated by anti-GPI-80 antibody treatment. However, similar to the GPI-80 family molecule, VNN1, the pantetheinase activity and oxidative state were augmented in GPI-80-expressing cells. GPI-80-expressing cells facilitated non-adhesive proliferation, slow cell proliferation, NF-κB activation and IL-1β production. These phenomena are known to be induced by physiological elevation of the oxidative state. Thus, these observations indicated that GPI-80 affects various tumor responses related to oxidation. |
| first_indexed | 2024-03-10T06:00:56Z |
| format | Article |
| id | doaj.art-a5810e6cad8b464db0454c85916ff062 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:00:56Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a5810e6cad8b464db0454c85916ff0622023-11-22T21:01:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122211202710.3390/ijms222112027GPI-80 Augments NF-κB Activation in Tumor CellsYuji Takeda0Yuta Kurota1Tomoyuki Kato2Hiromi Ito3Akemi Araki4Hidetoshi Nara5Shinichi Saitoh6Nobuyuki Tanaka7Norihiko Tsuchiya8Hironobu Asao9Department of Immunology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, JapanDepartment of Urology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, JapanDepartment of Urology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, JapanDepartment of Urology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, JapanDepartment of Immunology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, JapanDepartment of Immunology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, JapanDepartment of Immunology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, JapanDivision of Cancer Biology and Therapeutics, Miyagi Cancer Center Research Institute, Natori 981-1293, JapanDepartment of Urology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, JapanDepartment of Immunology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, JapanRecent studies have discovered a relationship between glycosylphosphatidylinositol (GPI)-anchored protein 80 (GPI-80)/VNN2 (80 kDa GPI-anchored protein) and malignant tumors. GPI-80 is known to regulate neutrophil adhesion; however, the action of GPI-80 on tumors is still obscure. In this study, although the expression of GPI-80 mRNA was detectable in several tumor cell lines, the levels of GPI-80 protein were significantly lower than that in neutrophils. To clarify the function of GPI-80 in tumor cells, GPI-80-expressing cells and GPI-80/VNN2 gene-deleted cells were established using PC3 prostate cancer cells. In GPI-80-expressing cells, GPI-80 was mainly detected in vesicles. Furthermore, soluble GPI-80 in the conditioned medium was associated with the exosome marker CD63 and was also detected in the plasma obtained from prostate cancer patients. Unexpectedly, cell adhesion and migration of GPI-80-expressing PC3 cells were not modulated by anti-GPI-80 antibody treatment. However, similar to the GPI-80 family molecule, VNN1, the pantetheinase activity and oxidative state were augmented in GPI-80-expressing cells. GPI-80-expressing cells facilitated non-adhesive proliferation, slow cell proliferation, NF-κB activation and IL-1β production. These phenomena are known to be induced by physiological elevation of the oxidative state. Thus, these observations indicated that GPI-80 affects various tumor responses related to oxidation.https://www.mdpi.com/1422-0067/22/21/12027IL-1βNF-κBoxidative stresspantetheinaseprostate cancer cells |
| spellingShingle | Yuji Takeda Yuta Kurota Tomoyuki Kato Hiromi Ito Akemi Araki Hidetoshi Nara Shinichi Saitoh Nobuyuki Tanaka Norihiko Tsuchiya Hironobu Asao GPI-80 Augments NF-κB Activation in Tumor Cells International Journal of Molecular Sciences IL-1β NF-κB oxidative stress pantetheinase prostate cancer cells |
| title | GPI-80 Augments NF-κB Activation in Tumor Cells |
| title_full | GPI-80 Augments NF-κB Activation in Tumor Cells |
| title_fullStr | GPI-80 Augments NF-κB Activation in Tumor Cells |
| title_full_unstemmed | GPI-80 Augments NF-κB Activation in Tumor Cells |
| title_short | GPI-80 Augments NF-κB Activation in Tumor Cells |
| title_sort | gpi 80 augments nf κb activation in tumor cells |
| topic | IL-1β NF-κB oxidative stress pantetheinase prostate cancer cells |
| url | https://www.mdpi.com/1422-0067/22/21/12027 |
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