Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains
The A. salmonicida A450 LPS O-antigen, encoded by the wbsalmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. It represents the first example of an O-antigen LPS polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. Until now...
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MDPI AG
2015-06-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/6/3791 |
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| author | Susana Merino Elena de Mendoza Rocío Canals Juan M. Tomás |
| author_facet | Susana Merino Elena de Mendoza Rocío Canals Juan M. Tomás |
| author_sort | Susana Merino |
| collection | DOAJ |
| description | The A. salmonicida A450 LPS O-antigen, encoded by the wbsalmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. It represents the first example of an O-antigen LPS polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. Until now, only repeating units with one or two different monosaccharides have been described. Functional genomic analysis of this wbsalmo region is mostly in agreement with the LPS O-antigen structure of acetylated l-rhamnose (Rha), d-glucose (Glc), and 2-amino-2-deoxy-d-mannose (ManN). Between genes of the wbsalmo we found the genes responsible for the biosynthesis and assembly of the S-layer (named A-layer in these strains). Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. pectinolytica strains, shared the same wbsalmo and presence of A-layer. A. salmonicida subsp. pectinolytica strains lack wbsalmo and A-layer, two major virulence factors, and this could be the reason they are the only ones not found as fish pathogens. |
| first_indexed | 2024-04-14T04:27:37Z |
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| id | doaj.art-a58225c7253d4a4484bb0977a38b19e9 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T04:27:37Z |
| publishDate | 2015-06-01 |
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| spelling | doaj.art-a58225c7253d4a4484bb0977a38b19e92022-12-22T02:12:15ZengMDPI AGMarine Drugs1660-33972015-06-011363791380810.3390/md13063791md13063791Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical StrainsSusana Merino0Elena de Mendoza1Rocío Canals2Juan M. Tomás3Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 643, Barcelona 08071, SpainDepartment of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 643, Barcelona 08071, SpainInstitute of Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UKDepartment of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 643, Barcelona 08071, SpainThe A. salmonicida A450 LPS O-antigen, encoded by the wbsalmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. It represents the first example of an O-antigen LPS polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. Until now, only repeating units with one or two different monosaccharides have been described. Functional genomic analysis of this wbsalmo region is mostly in agreement with the LPS O-antigen structure of acetylated l-rhamnose (Rha), d-glucose (Glc), and 2-amino-2-deoxy-d-mannose (ManN). Between genes of the wbsalmo we found the genes responsible for the biosynthesis and assembly of the S-layer (named A-layer in these strains). Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. pectinolytica strains, shared the same wbsalmo and presence of A-layer. A. salmonicida subsp. pectinolytica strains lack wbsalmo and A-layer, two major virulence factors, and this could be the reason they are the only ones not found as fish pathogens.http://www.mdpi.com/1660-3397/13/6/3791Aeromonas salmonicidasubspeciesgenomicsproteomicslipopolysaccharide O-antigenA-surface layer |
| spellingShingle | Susana Merino Elena de Mendoza Rocío Canals Juan M. Tomás Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains Marine Drugs Aeromonas salmonicida subspecies genomics proteomics lipopolysaccharide O-antigen A-surface layer |
| title | Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains |
| title_full | Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains |
| title_fullStr | Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains |
| title_full_unstemmed | Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains |
| title_short | Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains |
| title_sort | functional genomics of the aeromonas salmonicida lipopolysaccharide o antigen and a layer from typical and atypical strains |
| topic | Aeromonas salmonicida subspecies genomics proteomics lipopolysaccharide O-antigen A-surface layer |
| url | http://www.mdpi.com/1660-3397/13/6/3791 |
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