The Isopropyl Gallate Counteracts Cyclophosphamide-Induced Hemorrhagic Cystitis in Mice
Hemorrhagic cystitis is the main adverse effect associated with the clinical use of oxazaphosphorine, resulting in increased oxidative stress and proinflammatory cytokines, which culminate in injury of the bladder tissue. The aim of this study was to evaluate the protective effect of isopropyl galla...
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MDPI AG
2022-05-01
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| author | Lucas Solyano Almeida de Oliveira Sara Raquel de Moura Bandeira Rodrigo Lopes Gomes Gonçalves Benedito Pereira de Sousa Neto Diana Carvalho de Rezende Antonio Carlos dos Reis-Filho Ian Jhemes Oliveira Sousa Flaviano Ribeiro Pinheiro-Neto Boris Timah Acha Gabriela do Nascimento Caldas Trindade Lázaro Gomes do Nascimento Damião Pergentino de Sousa Fernanda Regina de Castro Almeida Massimo Lucarini Alessandra Durazzo Daniel Dias Rufino Arcanjo Francisco de Assis Oliveira |
| author_facet | Lucas Solyano Almeida de Oliveira Sara Raquel de Moura Bandeira Rodrigo Lopes Gomes Gonçalves Benedito Pereira de Sousa Neto Diana Carvalho de Rezende Antonio Carlos dos Reis-Filho Ian Jhemes Oliveira Sousa Flaviano Ribeiro Pinheiro-Neto Boris Timah Acha Gabriela do Nascimento Caldas Trindade Lázaro Gomes do Nascimento Damião Pergentino de Sousa Fernanda Regina de Castro Almeida Massimo Lucarini Alessandra Durazzo Daniel Dias Rufino Arcanjo Francisco de Assis Oliveira |
| author_sort | Lucas Solyano Almeida de Oliveira |
| collection | DOAJ |
| description | Hemorrhagic cystitis is the main adverse effect associated with the clinical use of oxazaphosphorine, resulting in increased oxidative stress and proinflammatory cytokines, which culminate in injury of the bladder tissue. The aim of this study was to evaluate the protective effect of isopropyl gallate (IPG) against ifosfamide (IFOS)-induced hemorrhagic cystitis in mice. The induction of the hemorrhagic cystitis model was carried out using a single dose of IFOS (400 mg/kg, i.p.) four hours after oral pretreatment with IPG (6.25, 12.5, 25, and 50 mg/kg) or saline (vehicle). Mesna (positive control; 80 mg/kg, i.p.) was administered four hours before and eight hours after induction of cystitis. In the present study, IPG 25 mg/kg significantly decreased edema and hemorrhage, with a reduction of the bladder wet weight (36.86%), hemoglobin content (54.55%), and peritoneal vascular permeability (42.94%) in urinary bladders of mice. Interestingly, IPG increased SOD activity (89.27%) and reduced MDA levels (35.53%), as well as displayed anti-inflammatory activity by decreasing TNF-α (88.77%), IL-1β (62.87%), and C-reactive protein (56.41%) levels. Our findings demonstrate that IPG has a substantial protective role against IFOS-induced hemorrhagic cystitis in mice by enhancing antioxidant activity and proinflammatory mechanisms. Thus, IPG represents a promising co-adjuvant agent in oxazaphosphorine-based chemotherapy treatments. |
| first_indexed | 2024-03-10T03:18:39Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T03:18:39Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj.art-a582bb5a76bd428d990ad32d25f4102c2023-11-23T10:07:36ZengMDPI AGBiology2079-77372022-05-0111572810.3390/biology11050728The Isopropyl Gallate Counteracts Cyclophosphamide-Induced Hemorrhagic Cystitis in MiceLucas Solyano Almeida de Oliveira0Sara Raquel de Moura Bandeira1Rodrigo Lopes Gomes Gonçalves2Benedito Pereira de Sousa Neto3Diana Carvalho de Rezende4Antonio Carlos dos Reis-Filho5Ian Jhemes Oliveira Sousa6Flaviano Ribeiro Pinheiro-Neto7Boris Timah Acha8Gabriela do Nascimento Caldas Trindade9Lázaro Gomes do Nascimento10Damião Pergentino de Sousa11Fernanda Regina de Castro Almeida12Massimo Lucarini13Alessandra Durazzo14Daniel Dias Rufino Arcanjo15Francisco de Assis Oliveira16Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilLaboratory of Pharmaceutical Chemistry, Federal University of Paraíba, João Pessoa 58051-900, BrazilLaboratory of Pharmaceutical Chemistry, Federal University of Paraíba, João Pessoa 58051-900, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilCREA-Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyCREA-Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, ItalyMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilMedicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina 64049-550, BrazilHemorrhagic cystitis is the main adverse effect associated with the clinical use of oxazaphosphorine, resulting in increased oxidative stress and proinflammatory cytokines, which culminate in injury of the bladder tissue. The aim of this study was to evaluate the protective effect of isopropyl gallate (IPG) against ifosfamide (IFOS)-induced hemorrhagic cystitis in mice. The induction of the hemorrhagic cystitis model was carried out using a single dose of IFOS (400 mg/kg, i.p.) four hours after oral pretreatment with IPG (6.25, 12.5, 25, and 50 mg/kg) or saline (vehicle). Mesna (positive control; 80 mg/kg, i.p.) was administered four hours before and eight hours after induction of cystitis. In the present study, IPG 25 mg/kg significantly decreased edema and hemorrhage, with a reduction of the bladder wet weight (36.86%), hemoglobin content (54.55%), and peritoneal vascular permeability (42.94%) in urinary bladders of mice. Interestingly, IPG increased SOD activity (89.27%) and reduced MDA levels (35.53%), as well as displayed anti-inflammatory activity by decreasing TNF-α (88.77%), IL-1β (62.87%), and C-reactive protein (56.41%) levels. Our findings demonstrate that IPG has a substantial protective role against IFOS-induced hemorrhagic cystitis in mice by enhancing antioxidant activity and proinflammatory mechanisms. Thus, IPG represents a promising co-adjuvant agent in oxazaphosphorine-based chemotherapy treatments.https://www.mdpi.com/2079-7737/11/5/728hemorrhagic cystitisifosfamideisopropyl gallateantineoplastic chemotherapy |
| spellingShingle | Lucas Solyano Almeida de Oliveira Sara Raquel de Moura Bandeira Rodrigo Lopes Gomes Gonçalves Benedito Pereira de Sousa Neto Diana Carvalho de Rezende Antonio Carlos dos Reis-Filho Ian Jhemes Oliveira Sousa Flaviano Ribeiro Pinheiro-Neto Boris Timah Acha Gabriela do Nascimento Caldas Trindade Lázaro Gomes do Nascimento Damião Pergentino de Sousa Fernanda Regina de Castro Almeida Massimo Lucarini Alessandra Durazzo Daniel Dias Rufino Arcanjo Francisco de Assis Oliveira The Isopropyl Gallate Counteracts Cyclophosphamide-Induced Hemorrhagic Cystitis in Mice Biology hemorrhagic cystitis ifosfamide isopropyl gallate antineoplastic chemotherapy |
| title | The Isopropyl Gallate Counteracts Cyclophosphamide-Induced Hemorrhagic Cystitis in Mice |
| title_full | The Isopropyl Gallate Counteracts Cyclophosphamide-Induced Hemorrhagic Cystitis in Mice |
| title_fullStr | The Isopropyl Gallate Counteracts Cyclophosphamide-Induced Hemorrhagic Cystitis in Mice |
| title_full_unstemmed | The Isopropyl Gallate Counteracts Cyclophosphamide-Induced Hemorrhagic Cystitis in Mice |
| title_short | The Isopropyl Gallate Counteracts Cyclophosphamide-Induced Hemorrhagic Cystitis in Mice |
| title_sort | isopropyl gallate counteracts cyclophosphamide induced hemorrhagic cystitis in mice |
| topic | hemorrhagic cystitis ifosfamide isopropyl gallate antineoplastic chemotherapy |
| url | https://www.mdpi.com/2079-7737/11/5/728 |
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