Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran
The present study was conducted as the first molecular detection of <em>Anaplasma</em> species in tick samples based on the sequencing of <em>major surface proteins 4 </em>(<em>msp4</em>) gene fragments in different parts of Iran. A total of 130 tick specimens wer...
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| Format: | Article |
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Razi Vaccine and Serum Research Institute
2020-03-01
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| Series: | Archives of Razi Institute |
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| Online Access: | https://archrazi.areeo.ac.ir/article_120979_80fca7dfc82c2975d27faf7e0ee176b1.pdf |
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| author | A. Hosseini-Chegeni M. Tavakoli Gh. Goudarzi Z. Telmadarraiy M. Sharifdini F. Faghihi M.K. Ghanbari |
| author_facet | A. Hosseini-Chegeni M. Tavakoli Gh. Goudarzi Z. Telmadarraiy M. Sharifdini F. Faghihi M.K. Ghanbari |
| author_sort | A. Hosseini-Chegeni |
| collection | DOAJ |
| description | The present study was conducted as the first molecular detection of <em>Anaplasma</em> species in tick samples based on the sequencing of <em>major surface proteins 4 </em>(<em>msp4</em>) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. <em>Hyalomma asiaticum</em>, <em>Hyalomma dromedarii</em>, <em>Rhipicephalus sanguineus</em>, and <em>Rhipicephalus </em>(<em>Boophilus</em>) species were identified in different geographical regions. An amplicon of 464-bp <em>msp4</em> of <em>Anaplasma</em> was amplified using polymerase chain reaction in various tick species. Three sequences, including one <em>Anaplasma marginale</em> from <em>R</em>. (<em>Boophilus</em>) species and two <em>Anaplasma ovis</em> from <em>Rhipicephalus sanguineus</em>, were obtained after sequencing. It is concluded that bovine and ovine anaplasmosis agents are present in tick samples in Iran. The use of the gene families of six major surface proteins for the detection of various Anaplasma species is recommended. |
| first_indexed | 2024-12-11T12:16:14Z |
| format | Article |
| id | doaj.art-a5845df0db7944298a92bd18b09ac957 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-11T12:16:14Z |
| publishDate | 2020-03-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-a5845df0db7944298a92bd18b09ac9572022-12-22T01:07:39ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722020-03-01751394610.22092/ari.2019.122532.1259120979Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in IranA. Hosseini-Chegeni0M. Tavakoli1Gh. Goudarzi2Z. Telmadarraiy3M. Sharifdini4F. Faghihi5M.K. Ghanbari6Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, IranAgricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural and Natural Resources Research Center, Khorramabad, IranDepartment of Medical Microbiology, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran| High Education Institute of Rahyan Novin Danesh (RND), Sari, IranDepartment of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, IranCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran| Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of National Program of Zoonotic Disease, School of Health Management and Information Sciences, Iran University of Medical Sciences, Tehran, IranThe present study was conducted as the first molecular detection of <em>Anaplasma</em> species in tick samples based on the sequencing of <em>major surface proteins 4 </em>(<em>msp4</em>) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. <em>Hyalomma asiaticum</em>, <em>Hyalomma dromedarii</em>, <em>Rhipicephalus sanguineus</em>, and <em>Rhipicephalus </em>(<em>Boophilus</em>) species were identified in different geographical regions. An amplicon of 464-bp <em>msp4</em> of <em>Anaplasma</em> was amplified using polymerase chain reaction in various tick species. Three sequences, including one <em>Anaplasma marginale</em> from <em>R</em>. (<em>Boophilus</em>) species and two <em>Anaplasma ovis</em> from <em>Rhipicephalus sanguineus</em>, were obtained after sequencing. It is concluded that bovine and ovine anaplasmosis agents are present in tick samples in Iran. The use of the gene families of six major surface proteins for the detection of various Anaplasma species is recommended.https://archrazi.areeo.ac.ir/article_120979_80fca7dfc82c2975d27faf7e0ee176b1.pdfanaplasmaphylogenetic treemsp4irantick |
| spellingShingle | A. Hosseini-Chegeni M. Tavakoli Gh. Goudarzi Z. Telmadarraiy M. Sharifdini F. Faghihi M.K. Ghanbari Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran Archives of Razi Institute anaplasma phylogenetic tree msp4 iran tick |
| title | Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran |
| title_full | Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran |
| title_fullStr | Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran |
| title_full_unstemmed | Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran |
| title_short | Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran |
| title_sort | molecular detection of anaplasma marginale and anaplasma ovis rickettsiales anaplasmataceae in ixodid tick species in iran |
| topic | anaplasma phylogenetic tree msp4 iran tick |
| url | https://archrazi.areeo.ac.ir/article_120979_80fca7dfc82c2975d27faf7e0ee176b1.pdf |
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