Renoprotection of Selected Antioxidant-Rich Foods (Water Spinach and Red Grape) and Probiotics in Gentamicin-Induced Nephrotoxicity and Oxidative Stress in Rats
Objectives: The current study investigated the curative effects of two selected antioxidant-rich foods (water spinach and red grape) and probiotics on the kidney exposed to nephrotoxicity induced by gentamicin. Methods: A total of 30 Wistar Albino female rats equally divided into six groups were stu...
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MDPI AG
2022-01-01
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| author | Sneha Sarwar Md. Jamal Hossain Nafis Md. Irfan Tamima Ahsan Md. Saidul Arefin Arebia Rahman Abdullah Alsubaie Badr Alharthi Mayeen Uddin Khandaker David A. Bradley Talha Bin Emran Sheikh Nazrul Islam |
| author_facet | Sneha Sarwar Md. Jamal Hossain Nafis Md. Irfan Tamima Ahsan Md. Saidul Arefin Arebia Rahman Abdullah Alsubaie Badr Alharthi Mayeen Uddin Khandaker David A. Bradley Talha Bin Emran Sheikh Nazrul Islam |
| author_sort | Sneha Sarwar |
| collection | DOAJ |
| description | Objectives: The current study investigated the curative effects of two selected antioxidant-rich foods (water spinach and red grape) and probiotics on the kidney exposed to nephrotoxicity induced by gentamicin. Methods: A total of 30 Wistar Albino female rats equally divided into six groups were studied for seven days. Except for the normal control (NC) group, all groups received 80 mg/kg/day gentamicin (GEN) injection intra-peritoneally for seven days. NC and GEN groups received only regular diet. In the water spinach group (GEN + WS) and red grape (GEN + RG) groups, rats were provided with 20 g/rat/day of boiled water spinach and 5 mL/rat/day of red grape juice, respectively. The probiotic (GEN + P<sub>4</sub>) and (GEN + P<sub>8</sub>) groups received 4 × 10<sup>9</sup> and 8 × 10<sup>9</sup> viable bacteria, respectively. On the 8th day, all the rats were sacrificed to collect blood and kidney. Serum creatinine, urea, uric acid, malondialdehyde (MDA), nitric oxide (NO), and superoxide dismutase (SOD) were analyzed. In addition, kidney histopathology was taken for final observation. Results: Both antioxidant-rich foods and probiotic (P<sub>4</sub>) significantly (<i>p</i> < 0.05) attenuated the GEN-induced oxidative and nitrosative stress and improved kidney function by lowering uremic toxin (serum creatinine, and uric acid) levels. Histopathological findings of kidney tissues of all groups were consistent with the biochemical findings. Conclusion: The current preclinical study suggests that the consumption of antioxidant-rich foods might be a promising fighting option against gentamycin-induced nephrotoxicity and oxidative stress. However, extensive studies and clinical monitoring are immediately required to determine the appropriate probiotic doses and mechanism of action for such effects. |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T01:07:37Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-a58da2ca5d084a2bad4e93f41724858a2023-11-23T14:24:19ZengMDPI AGLife2075-17292022-01-011216010.3390/life12010060Renoprotection of Selected Antioxidant-Rich Foods (Water Spinach and Red Grape) and Probiotics in Gentamicin-Induced Nephrotoxicity and Oxidative Stress in RatsSneha Sarwar0Md. Jamal Hossain1Nafis Md. Irfan2Tamima Ahsan3Md. Saidul Arefin4Arebia Rahman5Abdullah Alsubaie6Badr Alharthi7Mayeen Uddin Khandaker8David A. Bradley9Talha Bin Emran10Sheikh Nazrul Islam11Institute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, BangladeshDepartment of Pharmacy, State University of Bangladesh, 77 Satmasjid Road, Dhanmondi, Dhaka 1205, BangladeshInstitute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, BangladeshInstitute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, BangladeshInstitute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, BangladeshDepartment of Pathology, Dhaka Medical College and Hospital, Dhaka 1000, BangladeshDepartment of Physics, College of Khurma, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, University College of Al Khurmah, Taif University, PO. Box 11099, Taif 21944, Saudi ArabiaCentre for Applied Physics and Radiation Technologies, School of Engineering and Technology, Sunway University, Bandar Sunway, Petaling Jaya 47500, MalaysiaCentre for Applied Physics and Radiation Technologies, School of Engineering and Technology, Sunway University, Bandar Sunway, Petaling Jaya 47500, MalaysiaDepartment of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, BangladeshInstitute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, BangladeshObjectives: The current study investigated the curative effects of two selected antioxidant-rich foods (water spinach and red grape) and probiotics on the kidney exposed to nephrotoxicity induced by gentamicin. Methods: A total of 30 Wistar Albino female rats equally divided into six groups were studied for seven days. Except for the normal control (NC) group, all groups received 80 mg/kg/day gentamicin (GEN) injection intra-peritoneally for seven days. NC and GEN groups received only regular diet. In the water spinach group (GEN + WS) and red grape (GEN + RG) groups, rats were provided with 20 g/rat/day of boiled water spinach and 5 mL/rat/day of red grape juice, respectively. The probiotic (GEN + P<sub>4</sub>) and (GEN + P<sub>8</sub>) groups received 4 × 10<sup>9</sup> and 8 × 10<sup>9</sup> viable bacteria, respectively. On the 8th day, all the rats were sacrificed to collect blood and kidney. Serum creatinine, urea, uric acid, malondialdehyde (MDA), nitric oxide (NO), and superoxide dismutase (SOD) were analyzed. In addition, kidney histopathology was taken for final observation. Results: Both antioxidant-rich foods and probiotic (P<sub>4</sub>) significantly (<i>p</i> < 0.05) attenuated the GEN-induced oxidative and nitrosative stress and improved kidney function by lowering uremic toxin (serum creatinine, and uric acid) levels. Histopathological findings of kidney tissues of all groups were consistent with the biochemical findings. Conclusion: The current preclinical study suggests that the consumption of antioxidant-rich foods might be a promising fighting option against gentamycin-induced nephrotoxicity and oxidative stress. However, extensive studies and clinical monitoring are immediately required to determine the appropriate probiotic doses and mechanism of action for such effects.https://www.mdpi.com/2075-1729/12/1/60nephroprotectionwater spinachred grapeprobioticsgentamicin-induced nephropathyoxidative stress |
| spellingShingle | Sneha Sarwar Md. Jamal Hossain Nafis Md. Irfan Tamima Ahsan Md. Saidul Arefin Arebia Rahman Abdullah Alsubaie Badr Alharthi Mayeen Uddin Khandaker David A. Bradley Talha Bin Emran Sheikh Nazrul Islam Renoprotection of Selected Antioxidant-Rich Foods (Water Spinach and Red Grape) and Probiotics in Gentamicin-Induced Nephrotoxicity and Oxidative Stress in Rats Life nephroprotection water spinach red grape probiotics gentamicin-induced nephropathy oxidative stress |
| title | Renoprotection of Selected Antioxidant-Rich Foods (Water Spinach and Red Grape) and Probiotics in Gentamicin-Induced Nephrotoxicity and Oxidative Stress in Rats |
| title_full | Renoprotection of Selected Antioxidant-Rich Foods (Water Spinach and Red Grape) and Probiotics in Gentamicin-Induced Nephrotoxicity and Oxidative Stress in Rats |
| title_fullStr | Renoprotection of Selected Antioxidant-Rich Foods (Water Spinach and Red Grape) and Probiotics in Gentamicin-Induced Nephrotoxicity and Oxidative Stress in Rats |
| title_full_unstemmed | Renoprotection of Selected Antioxidant-Rich Foods (Water Spinach and Red Grape) and Probiotics in Gentamicin-Induced Nephrotoxicity and Oxidative Stress in Rats |
| title_short | Renoprotection of Selected Antioxidant-Rich Foods (Water Spinach and Red Grape) and Probiotics in Gentamicin-Induced Nephrotoxicity and Oxidative Stress in Rats |
| title_sort | renoprotection of selected antioxidant rich foods water spinach and red grape and probiotics in gentamicin induced nephrotoxicity and oxidative stress in rats |
| topic | nephroprotection water spinach red grape probiotics gentamicin-induced nephropathy oxidative stress |
| url | https://www.mdpi.com/2075-1729/12/1/60 |
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