A Comparison of Oral and Intravenous Mouse Models of Listeriosis
Listeria monocytogenes is one of several enteric microbes that is acquired orally, invades the gastric mucosa, and then disseminates to peripheral tissues to cause systemic disease in humans. Intravenous (i.v.) inoculation of mice with L. monocytogenes has been the most widely-used small animal mode...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Pathogens |
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| Online Access: | http://www.mdpi.com/2076-0817/7/1/13 |
| _version_ | 1818038968562745344 |
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| author | Michelle G. Pitts Sarah E. F. D’Orazio |
| author_facet | Michelle G. Pitts Sarah E. F. D’Orazio |
| author_sort | Michelle G. Pitts |
| collection | DOAJ |
| description | Listeria monocytogenes is one of several enteric microbes that is acquired orally, invades the gastric mucosa, and then disseminates to peripheral tissues to cause systemic disease in humans. Intravenous (i.v.) inoculation of mice with L. monocytogenes has been the most widely-used small animal model of listeriosis over the past few decades. The infection is highly reproducible and has been invaluable in deciphering mechanisms of adaptive immunity in vivo, particularly CD8+ T cell responses to intracellular pathogens. However, the i.v. model completely bypasses the gut phase of the infection. Recent advances in generating both humanized mice and murinized bacteria, as well as the development of a foodborne route of transmission has reignited interest in studying oral models of listeriosis. In this review, we analyze previously published reports to highlight both the similarities and differences in tissue colonization and host response to infection using either oral or i.v. inoculation. |
| first_indexed | 2024-12-10T07:51:10Z |
| format | Article |
| id | doaj.art-a58e3fbdc14842c089e8ccf6df39065c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-12-10T07:51:10Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-a58e3fbdc14842c089e8ccf6df39065c2022-12-22T01:57:02ZengMDPI AGPathogens2076-08172018-01-01711310.3390/pathogens7010013pathogens7010013A Comparison of Oral and Intravenous Mouse Models of ListeriosisMichelle G. Pitts0Sarah E. F. D’Orazio1Department of Microbiology, Immunology & Molecular Genetics, University of Kentucky, 800 Rose Street–MS417, Lexington, KY 40536-0298, USADepartment of Microbiology, Immunology & Molecular Genetics, University of Kentucky, 800 Rose Street–MS417, Lexington, KY 40536-0298, USAListeria monocytogenes is one of several enteric microbes that is acquired orally, invades the gastric mucosa, and then disseminates to peripheral tissues to cause systemic disease in humans. Intravenous (i.v.) inoculation of mice with L. monocytogenes has been the most widely-used small animal model of listeriosis over the past few decades. The infection is highly reproducible and has been invaluable in deciphering mechanisms of adaptive immunity in vivo, particularly CD8+ T cell responses to intracellular pathogens. However, the i.v. model completely bypasses the gut phase of the infection. Recent advances in generating both humanized mice and murinized bacteria, as well as the development of a foodborne route of transmission has reignited interest in studying oral models of listeriosis. In this review, we analyze previously published reports to highlight both the similarities and differences in tissue colonization and host response to infection using either oral or i.v. inoculation.http://www.mdpi.com/2076-0817/7/1/13Listeria monocytogenesintracellular bacteriafoodborne pathogenhost susceptibilityvirulence |
| spellingShingle | Michelle G. Pitts Sarah E. F. D’Orazio A Comparison of Oral and Intravenous Mouse Models of Listeriosis Pathogens Listeria monocytogenes intracellular bacteria foodborne pathogen host susceptibility virulence |
| title | A Comparison of Oral and Intravenous Mouse Models of Listeriosis |
| title_full | A Comparison of Oral and Intravenous Mouse Models of Listeriosis |
| title_fullStr | A Comparison of Oral and Intravenous Mouse Models of Listeriosis |
| title_full_unstemmed | A Comparison of Oral and Intravenous Mouse Models of Listeriosis |
| title_short | A Comparison of Oral and Intravenous Mouse Models of Listeriosis |
| title_sort | comparison of oral and intravenous mouse models of listeriosis |
| topic | Listeria monocytogenes intracellular bacteria foodborne pathogen host susceptibility virulence |
| url | http://www.mdpi.com/2076-0817/7/1/13 |
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