Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes progression and metastasis of laryngeal squamous cell carcinoma
Abstract Background Laryngeal squamous cell carcinoma (LSCC) is among the most common malignant tumors with poor prognosis. Accumulating evidences have identified the important roles of long noncoding RNAs (lncRNAs) in the initiation and progression of various cancer types; however, the global lncRN...
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Epigenetics & Chromatin |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13072-019-0283-8 |
| _version_ | 1819207381300543488 |
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| author | Baoshan Wang Lei Zhao Weiwei Chi Huan Cao Weina Cui Wenxia Meng |
| author_facet | Baoshan Wang Lei Zhao Weiwei Chi Huan Cao Weina Cui Wenxia Meng |
| author_sort | Baoshan Wang |
| collection | DOAJ |
| description | Abstract Background Laryngeal squamous cell carcinoma (LSCC) is among the most common malignant tumors with poor prognosis. Accumulating evidences have identified the important roles of long noncoding RNAs (lncRNAs) in the initiation and progression of various cancer types; however, the global lncRNAs expression profile for metastatic LSCC is limited. Results In the present study, we screen expression profiles of lncRNAs in advanced LSCC patients with paired tumor tissues and corresponding normal tissues by microarrays. We identify numerous differentially expressed transcripts, and after the necessary verification of the transcripts expression in expanded samples, we experimentally validate the expression patterns of the remarkable low expressed gene, SSTR5, and its antisense lncRNA, SSTR5-AS1. Downregulation of SSTR5 is detected in LSCC tissues and laryngeal carcinoma cells. Aberrant DNA hypermethylation of the CpG sites clustered in the exon 1 and accumulation of inactive histone modifications at SSTR5 promoter region may be epigenetic mechanisms for its inactivation in LSCC. SSTR5-AS1 may play antitumor role in LSCC and may be regulated by the hypermethylation of the same CpG sites with SSTR5. SSTR5-AS1 inhibits laryngeal carcinoma cells proliferation, migration, and invasion. SSTR5-AS1 increases the enrichment of MLL3 and H3K4me3 at the promoter region of SSTR5 by interacting with MLL3 and further induces the transcription of SSTR5. Furthermore, SSTR5-AS1 interacts with and recruits TET1 to its target gene E-cadherin to activate its expression. Conclusion These findings suggest that the identified lncRNAs and mRNAs may be potential biomarkers in metastatic LSCC, and SSTR5-AS1 may act as a tumor suppressor as well as a potential biomarker for antitumor therapy. |
| first_indexed | 2024-12-23T05:22:35Z |
| format | Article |
| id | doaj.art-a5909694476f448fb7a4b4185b6e0503 |
| institution | Directory Open Access Journal |
| issn | 1756-8935 |
| language | English |
| last_indexed | 2024-12-23T05:22:35Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Epigenetics & Chromatin |
| spelling | doaj.art-a5909694476f448fb7a4b4185b6e05032022-12-21T17:58:39ZengBMCEpigenetics & Chromatin1756-89352019-06-0112111710.1186/s13072-019-0283-8Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes progression and metastasis of laryngeal squamous cell carcinomaBaoshan Wang0Lei Zhao1Weiwei Chi2Huan Cao3Weina Cui4Wenxia Meng5Otolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei Medical UniversityOtolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei Medical UniversityOtolaryngology Head and Neck Surgery Department, The First Hospital of Hebei Medical UniversityOtolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei Medical UniversityOtolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei Medical UniversityOtolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei Medical UniversityAbstract Background Laryngeal squamous cell carcinoma (LSCC) is among the most common malignant tumors with poor prognosis. Accumulating evidences have identified the important roles of long noncoding RNAs (lncRNAs) in the initiation and progression of various cancer types; however, the global lncRNAs expression profile for metastatic LSCC is limited. Results In the present study, we screen expression profiles of lncRNAs in advanced LSCC patients with paired tumor tissues and corresponding normal tissues by microarrays. We identify numerous differentially expressed transcripts, and after the necessary verification of the transcripts expression in expanded samples, we experimentally validate the expression patterns of the remarkable low expressed gene, SSTR5, and its antisense lncRNA, SSTR5-AS1. Downregulation of SSTR5 is detected in LSCC tissues and laryngeal carcinoma cells. Aberrant DNA hypermethylation of the CpG sites clustered in the exon 1 and accumulation of inactive histone modifications at SSTR5 promoter region may be epigenetic mechanisms for its inactivation in LSCC. SSTR5-AS1 may play antitumor role in LSCC and may be regulated by the hypermethylation of the same CpG sites with SSTR5. SSTR5-AS1 inhibits laryngeal carcinoma cells proliferation, migration, and invasion. SSTR5-AS1 increases the enrichment of MLL3 and H3K4me3 at the promoter region of SSTR5 by interacting with MLL3 and further induces the transcription of SSTR5. Furthermore, SSTR5-AS1 interacts with and recruits TET1 to its target gene E-cadherin to activate its expression. Conclusion These findings suggest that the identified lncRNAs and mRNAs may be potential biomarkers in metastatic LSCC, and SSTR5-AS1 may act as a tumor suppressor as well as a potential biomarker for antitumor therapy.http://link.springer.com/article/10.1186/s13072-019-0283-8SSTR5SSTR5-AS1Laryngeal squamous cell carcinomaExpressionMethylation |
| spellingShingle | Baoshan Wang Lei Zhao Weiwei Chi Huan Cao Weina Cui Wenxia Meng Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes progression and metastasis of laryngeal squamous cell carcinoma Epigenetics & Chromatin SSTR5 SSTR5-AS1 Laryngeal squamous cell carcinoma Expression Methylation |
| title | Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes progression and metastasis of laryngeal squamous cell carcinoma |
| title_full | Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes progression and metastasis of laryngeal squamous cell carcinoma |
| title_fullStr | Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes progression and metastasis of laryngeal squamous cell carcinoma |
| title_full_unstemmed | Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes progression and metastasis of laryngeal squamous cell carcinoma |
| title_short | Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes progression and metastasis of laryngeal squamous cell carcinoma |
| title_sort | aberrant methylation mediated downregulation of lncrna sstr5 as1 promotes progression and metastasis of laryngeal squamous cell carcinoma |
| topic | SSTR5 SSTR5-AS1 Laryngeal squamous cell carcinoma Expression Methylation |
| url | http://link.springer.com/article/10.1186/s13072-019-0283-8 |
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