Enzyme-Treated <i>Zizania latifolia</i> Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation
Ultraviolet A (UVA) is a risk factor for photoaging and wrinkle formation. <i>Zizania latifolia</i> is an herbaceous perennial plant. It contains many bioactive compounds such as tricin that show antioxidative and anti-inflammatory effects. The aim of this study was to investigate the an...
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MDPI AG
2020-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/10/912 |
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| author | Mirae An Hyungkeun Kim Joo-Myung Moon Hyun-Soo Ko Paul Clayton Young-Hee Lim |
| author_facet | Mirae An Hyungkeun Kim Joo-Myung Moon Hyun-Soo Ko Paul Clayton Young-Hee Lim |
| author_sort | Mirae An |
| collection | DOAJ |
| description | Ultraviolet A (UVA) is a risk factor for photoaging and wrinkle formation. <i>Zizania latifolia</i> is an herbaceous perennial plant. It contains many bioactive compounds such as tricin that show antioxidative and anti-inflammatory effects. The aim of this study was to investigate the antiwrinkle effect of a mixture of hydrolytic enzyme (cellulase, hemicellulase and pectinase)-treated <i>Z. latifolia</i> extract (ZLE) and tricin on UVA-irradiated human dermal fibroblasts (HDFs) and SKH-1 hairless mice. Treatment of UVA-irradiated HDF cells with ZLE and tricin significantly decreased UVA induced-plasma membrane rupture, generation of ROS, expression levels of total and secreted lysosomal associated membrane protein (LAMP-1), cathepsin B and metalloproteinases (MMPs) and inhibited NF-κB activation. In the animal study, UVA-damaged epidermal and dermal tissues were repaired by the ZLE and tricin treatments. Administration of ZLE or tricin to UVA-irradiated animals recovered skin surface moisture and collagen fiber in dermal tissue. Treatment of ZLE or tricin decreased wrinkle formation, secretion of MMPs and expression levels of vascular endothelial growth factor (VEGF) and cathepsin B, and increased the expression level of collagen-1 in UVA-irradiated animals. Overall, the ZLE and tricin treatments decreased the skin damage induced by UVA irradiation via inhibition of lysosomal exocytosis and ROS generation. Therefore, ZLE and tricin are promising as antiwrinkle and antiphotoaging agents. |
| first_indexed | 2024-03-10T16:04:55Z |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T16:04:55Z |
| publishDate | 2020-09-01 |
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| series | Antioxidants |
| spelling | doaj.art-a591fbb1e26f4fe195ef5c88678c17872023-11-20T14:58:42ZengMDPI AGAntioxidants2076-39212020-09-0191091210.3390/antiox9100912Enzyme-Treated <i>Zizania latifolia</i> Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species GenerationMirae An0Hyungkeun Kim1Joo-Myung Moon2Hyun-Soo Ko3Paul Clayton4Young-Hee Lim5Department of Public Health Science (BK21 PLUS Program), Graduate School, Korea University, Seoul 02841, KoreaDepartment of Oral Biology, Oral Cancer Research Institute (BK21 PLUS Program), Yonsei University College of Dentistry, Seoul 03722, KoreaBTC Corporation, Ansan, Gyeonggi-do 15588, KoreaDepartment of Integrated Biomedical and Life Sciences, Graduate School, Korea University, Seoul 02841, KoreaInstitute of Food, Brain and Behaviour, Beaver House, 23-38 Hyde Bridge Street, Oxford OX1 2EP, UKDepartment of Public Health Science (BK21 PLUS Program), Graduate School, Korea University, Seoul 02841, KoreaUltraviolet A (UVA) is a risk factor for photoaging and wrinkle formation. <i>Zizania latifolia</i> is an herbaceous perennial plant. It contains many bioactive compounds such as tricin that show antioxidative and anti-inflammatory effects. The aim of this study was to investigate the antiwrinkle effect of a mixture of hydrolytic enzyme (cellulase, hemicellulase and pectinase)-treated <i>Z. latifolia</i> extract (ZLE) and tricin on UVA-irradiated human dermal fibroblasts (HDFs) and SKH-1 hairless mice. Treatment of UVA-irradiated HDF cells with ZLE and tricin significantly decreased UVA induced-plasma membrane rupture, generation of ROS, expression levels of total and secreted lysosomal associated membrane protein (LAMP-1), cathepsin B and metalloproteinases (MMPs) and inhibited NF-κB activation. In the animal study, UVA-damaged epidermal and dermal tissues were repaired by the ZLE and tricin treatments. Administration of ZLE or tricin to UVA-irradiated animals recovered skin surface moisture and collagen fiber in dermal tissue. Treatment of ZLE or tricin decreased wrinkle formation, secretion of MMPs and expression levels of vascular endothelial growth factor (VEGF) and cathepsin B, and increased the expression level of collagen-1 in UVA-irradiated animals. Overall, the ZLE and tricin treatments decreased the skin damage induced by UVA irradiation via inhibition of lysosomal exocytosis and ROS generation. Therefore, ZLE and tricin are promising as antiwrinkle and antiphotoaging agents.https://www.mdpi.com/2076-3921/9/10/912antiwrinklelysosomal sheddingmatrix metalloproteinasesultraviolet A<i>Zizania latifolia</i> |
| spellingShingle | Mirae An Hyungkeun Kim Joo-Myung Moon Hyun-Soo Ko Paul Clayton Young-Hee Lim Enzyme-Treated <i>Zizania latifolia</i> Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation Antioxidants antiwrinkle lysosomal shedding matrix metalloproteinases ultraviolet A <i>Zizania latifolia</i> |
| title | Enzyme-Treated <i>Zizania latifolia</i> Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation |
| title_full | Enzyme-Treated <i>Zizania latifolia</i> Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation |
| title_fullStr | Enzyme-Treated <i>Zizania latifolia</i> Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation |
| title_full_unstemmed | Enzyme-Treated <i>Zizania latifolia</i> Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation |
| title_short | Enzyme-Treated <i>Zizania latifolia</i> Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation |
| title_sort | enzyme treated i zizania latifolia i ethanol extract protects from uva irradiation induced wrinkle formation via inhibition of lysosome exocytosis and reactive oxygen species generation |
| topic | antiwrinkle lysosomal shedding matrix metalloproteinases ultraviolet A <i>Zizania latifolia</i> |
| url | https://www.mdpi.com/2076-3921/9/10/912 |
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