Amitriptyline Effects on Pentylentetrazole-Induced Oxidative Stress in Epileptic Male Wistar Rats
Background: Drug-induced epilepsy can be caused by certain medications and chemical agents. Oxidizing agents are among the chemical compounds that cause epilepsy. The aim of this study was to evaluate the anticonvulsant and antioxidant effects of amitriptyline on pentylenetetrazole (PTZ)-induced sei...
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2022-05-01
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| Series: | Iranian South Medical Journal |
| Subjects: | |
| Online Access: | http://ismj.bpums.ac.ir/article-1-1611-en.html |
| _version_ | 1811241973432975360 |
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| author | Naser Mirazi Soheila Niyazi Abdolkarim Hosseini Zahra Izadi |
| author_facet | Naser Mirazi Soheila Niyazi Abdolkarim Hosseini Zahra Izadi |
| author_sort | Naser Mirazi |
| collection | DOAJ |
| description | Background: Drug-induced epilepsy can be caused by certain medications and chemical agents. Oxidizing agents are among the chemical compounds that cause epilepsy. The aim of this study was to evaluate the anticonvulsant and antioxidant effects of amitriptyline on pentylenetetrazole (PTZ)-induced seizure in male rats.
Materials and Methods: In this experimental study, 28 male Wistar rats (200-250 g) were used. Rats were randomly divided into four groups (n=7): Control (0.5 ml normal saline), PTZ (PTZ, 60 mg/kg), treatment group 1 (PTZ, 60 mg/kg + amitriptyline, 10 mg/kg), treatment group 2 (PTZ, 60 mg/kg+ amitriptyline, 20 mg/kg). All injections were intraperitoneal. At the end of the experiments, the rats were anesthetized with ketamine hydrochloride (50 mg/kg) + xylazine (10 mg/kg) and their blood samples were directly drawn from the heart and then the sera were isolated. Oxidative stress markers such as GPx, MDA, NO and GSH were measured. The results were statistically analyzed using one-way ANOVA and Tukey’s test to examine the differences between the groups.
Results: The results of this study showed that the delay in myoclonic jerks increased in the
amitriptyline-treated groups compared to the PTZ group. Also, the delay of general tonic-clonic seizures increased in the treated groups compared to the PTZ group. In addition, serum levels of GSH and GPx in the PTZ group showed a significant decrease compared to the control group. Serum levels of MDA and NO in the PTZ group showed a significant increase compared to the control group.
Conclusion: Amitriptyline is able to reduce the seizures induced by PTZ injection in rats. Also, it showed a suppressing effect on some oxidizing agents in this study. |
| first_indexed | 2024-04-12T13:43:58Z |
| format | Article |
| id | doaj.art-a5950f6067174683a757397d1049828a |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-04-12T13:43:58Z |
| publishDate | 2022-05-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-a5950f6067174683a757397d1049828a2022-12-22T03:30:44ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542022-05-0125293104Amitriptyline Effects on Pentylentetrazole-Induced Oxidative Stress in Epileptic Male Wistar RatsNaser Mirazi0Soheila Niyazi1Abdolkarim Hosseini2Zahra Izadi3 Department of Biology, School of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran Department of Biology, School of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran Department of Physiology, School of Basic Sciences, Shahid Beheshti University, Tehran, Iran Department of Horticultural Sciences and Engineering, Nahavand Higher Education Complex, Bu-Ali Sina University, Hamedan, Iran Background: Drug-induced epilepsy can be caused by certain medications and chemical agents. Oxidizing agents are among the chemical compounds that cause epilepsy. The aim of this study was to evaluate the anticonvulsant and antioxidant effects of amitriptyline on pentylenetetrazole (PTZ)-induced seizure in male rats. Materials and Methods: In this experimental study, 28 male Wistar rats (200-250 g) were used. Rats were randomly divided into four groups (n=7): Control (0.5 ml normal saline), PTZ (PTZ, 60 mg/kg), treatment group 1 (PTZ, 60 mg/kg + amitriptyline, 10 mg/kg), treatment group 2 (PTZ, 60 mg/kg+ amitriptyline, 20 mg/kg). All injections were intraperitoneal. At the end of the experiments, the rats were anesthetized with ketamine hydrochloride (50 mg/kg) + xylazine (10 mg/kg) and their blood samples were directly drawn from the heart and then the sera were isolated. Oxidative stress markers such as GPx, MDA, NO and GSH were measured. The results were statistically analyzed using one-way ANOVA and Tukey’s test to examine the differences between the groups. Results: The results of this study showed that the delay in myoclonic jerks increased in the amitriptyline-treated groups compared to the PTZ group. Also, the delay of general tonic-clonic seizures increased in the treated groups compared to the PTZ group. In addition, serum levels of GSH and GPx in the PTZ group showed a significant decrease compared to the control group. Serum levels of MDA and NO in the PTZ group showed a significant increase compared to the control group. Conclusion: Amitriptyline is able to reduce the seizures induced by PTZ injection in rats. Also, it showed a suppressing effect on some oxidizing agents in this study.http://ismj.bpums.ac.ir/article-1-1611-en.htmlamitriptylineantioxidantepilepsyglutathione peroxidasepentylentetrazolemalondialdehyde |
| spellingShingle | Naser Mirazi Soheila Niyazi Abdolkarim Hosseini Zahra Izadi Amitriptyline Effects on Pentylentetrazole-Induced Oxidative Stress in Epileptic Male Wistar Rats Iranian South Medical Journal amitriptyline antioxidant epilepsy glutathione peroxidase pentylentetrazole malondialdehyde |
| title | Amitriptyline Effects on Pentylentetrazole-Induced Oxidative Stress in Epileptic Male Wistar Rats |
| title_full | Amitriptyline Effects on Pentylentetrazole-Induced Oxidative Stress in Epileptic Male Wistar Rats |
| title_fullStr | Amitriptyline Effects on Pentylentetrazole-Induced Oxidative Stress in Epileptic Male Wistar Rats |
| title_full_unstemmed | Amitriptyline Effects on Pentylentetrazole-Induced Oxidative Stress in Epileptic Male Wistar Rats |
| title_short | Amitriptyline Effects on Pentylentetrazole-Induced Oxidative Stress in Epileptic Male Wistar Rats |
| title_sort | amitriptyline effects on pentylentetrazole induced oxidative stress in epileptic male wistar rats |
| topic | amitriptyline antioxidant epilepsy glutathione peroxidase pentylentetrazole malondialdehyde |
| url | http://ismj.bpums.ac.ir/article-1-1611-en.html |
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