Fecal microbiota in premature infants prior to necrotizing enterocolitis.
Intestinal luminal microbiota likely contribute to the etiology of necrotizing enterocolitis (NEC), a common disease in preterm infants. Microbiota development, a cascade of initial colonization events leading to the establishment of a diverse commensal microbiota, can now be studied in preterm infa...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3108958?pdf=render |
| _version_ | 1811192205320126464 |
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| author | Volker Mai Christopher Michael Young Maria Ukhanova Xiaoyu Wang Yijun Sun George Casella Douglas Theriaque Nan Li Renu Sharma Mark Hudak Josef Neu |
| author_facet | Volker Mai Christopher Michael Young Maria Ukhanova Xiaoyu Wang Yijun Sun George Casella Douglas Theriaque Nan Li Renu Sharma Mark Hudak Josef Neu |
| author_sort | Volker Mai |
| collection | DOAJ |
| description | Intestinal luminal microbiota likely contribute to the etiology of necrotizing enterocolitis (NEC), a common disease in preterm infants. Microbiota development, a cascade of initial colonization events leading to the establishment of a diverse commensal microbiota, can now be studied in preterm infants using powerful molecular tools. Starting with the first stool and continuing until discharge, weekly stool specimens were collected prospectively from infants with gestational ages ≤32 completed weeks or birth weights≤1250 g. High throughput 16S rRNA sequencing was used to compare the diversity of microbiota and the prevalence of specific bacterial signatures in nine NEC infants and in nine matched controls. After removal of short and low quality reads we retained a total of 110,021 sequences. Microbiota composition differed in the matched samples collected 1 week but not <72 hours prior to NEC diagnosis. We detected a bloom (34% increase) of Proteobacteria and a decrease (32%) in Firmicutes in NEC cases between the 1 week and <72 hour samples. No significant change was identified in the controls. At both time points, molecular signatures were identified that were increased in NEC cases. One of the bacterial signatures detected more frequently in NEC cases (p<0.01) matched closest to γ-Proteobacteria. Although this sequence grouped to the well-studied Enterobacteriaceae family, it did not match any sequence in Genbank by more than 97%. Our observations suggest that abnormal patterns of microbiota and potentially a novel pathogen contribute to the etiology of NEC. |
| first_indexed | 2024-04-11T23:48:53Z |
| format | Article |
| id | doaj.art-a59bdf32cc1e4970a15923aa3a25499d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T23:48:53Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a59bdf32cc1e4970a15923aa3a25499d2022-12-22T03:56:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2064710.1371/journal.pone.0020647Fecal microbiota in premature infants prior to necrotizing enterocolitis.Volker MaiChristopher Michael YoungMaria UkhanovaXiaoyu WangYijun SunGeorge CasellaDouglas TheriaqueNan LiRenu SharmaMark HudakJosef NeuIntestinal luminal microbiota likely contribute to the etiology of necrotizing enterocolitis (NEC), a common disease in preterm infants. Microbiota development, a cascade of initial colonization events leading to the establishment of a diverse commensal microbiota, can now be studied in preterm infants using powerful molecular tools. Starting with the first stool and continuing until discharge, weekly stool specimens were collected prospectively from infants with gestational ages ≤32 completed weeks or birth weights≤1250 g. High throughput 16S rRNA sequencing was used to compare the diversity of microbiota and the prevalence of specific bacterial signatures in nine NEC infants and in nine matched controls. After removal of short and low quality reads we retained a total of 110,021 sequences. Microbiota composition differed in the matched samples collected 1 week but not <72 hours prior to NEC diagnosis. We detected a bloom (34% increase) of Proteobacteria and a decrease (32%) in Firmicutes in NEC cases between the 1 week and <72 hour samples. No significant change was identified in the controls. At both time points, molecular signatures were identified that were increased in NEC cases. One of the bacterial signatures detected more frequently in NEC cases (p<0.01) matched closest to γ-Proteobacteria. Although this sequence grouped to the well-studied Enterobacteriaceae family, it did not match any sequence in Genbank by more than 97%. Our observations suggest that abnormal patterns of microbiota and potentially a novel pathogen contribute to the etiology of NEC.http://europepmc.org/articles/PMC3108958?pdf=render |
| spellingShingle | Volker Mai Christopher Michael Young Maria Ukhanova Xiaoyu Wang Yijun Sun George Casella Douglas Theriaque Nan Li Renu Sharma Mark Hudak Josef Neu Fecal microbiota in premature infants prior to necrotizing enterocolitis. PLoS ONE |
| title | Fecal microbiota in premature infants prior to necrotizing enterocolitis. |
| title_full | Fecal microbiota in premature infants prior to necrotizing enterocolitis. |
| title_fullStr | Fecal microbiota in premature infants prior to necrotizing enterocolitis. |
| title_full_unstemmed | Fecal microbiota in premature infants prior to necrotizing enterocolitis. |
| title_short | Fecal microbiota in premature infants prior to necrotizing enterocolitis. |
| title_sort | fecal microbiota in premature infants prior to necrotizing enterocolitis |
| url | http://europepmc.org/articles/PMC3108958?pdf=render |
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