A clearing-free protocol for imaging intact whole adipose tissue innervation in mice
Summary: Here we provide a clearing-free protocol for processing intact, whole mount subcutaneous white adipose tissue (scWAT) for immunofluorescence as an alternative to current clearing-based approaches. We use a combination of Z-depth reduction and autofluorescence quenching techniques to fluores...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721008157 |
| _version_ | 1828882618298400768 |
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| author | Jake W. Willows Magdalena Blaszkiewicz Kristy L. Townsend |
| author_facet | Jake W. Willows Magdalena Blaszkiewicz Kristy L. Townsend |
| author_sort | Jake W. Willows |
| collection | DOAJ |
| description | Summary: Here we provide a clearing-free protocol for processing intact, whole mount subcutaneous white adipose tissue (scWAT) for immunofluorescence as an alternative to current clearing-based approaches. We use a combination of Z-depth reduction and autofluorescence quenching techniques to fluorescently label, image, and quantify adipose tissue innervation effectively throughout intact mouse tissues without the need for optical clearing or light sheet microscopy. This protocol has been optimized and validated for adipose neurovascular labeling.For complete details on the use and execution of this protocol, please refer to Willows et al. (2021). |
| first_indexed | 2024-12-13T10:31:39Z |
| format | Article |
| id | doaj.art-a5a0fe9c6a484fdaa90b7e5bc4fd5413 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T10:31:39Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-a5a0fe9c6a484fdaa90b7e5bc4fd54132022-12-21T23:50:50ZengElsevierSTAR Protocols2666-16672022-03-0131101109A clearing-free protocol for imaging intact whole adipose tissue innervation in miceJake W. Willows0Magdalena Blaszkiewicz1Kristy L. Townsend2Department of Neurological Surgery, The Ohio State University, Columbus, OH 43210, USADepartment of Neurological Surgery, The Ohio State University, Columbus, OH 43210, USADepartment of Neurological Surgery, The Ohio State University, Columbus, OH 43210, USA; Corresponding authorSummary: Here we provide a clearing-free protocol for processing intact, whole mount subcutaneous white adipose tissue (scWAT) for immunofluorescence as an alternative to current clearing-based approaches. We use a combination of Z-depth reduction and autofluorescence quenching techniques to fluorescently label, image, and quantify adipose tissue innervation effectively throughout intact mouse tissues without the need for optical clearing or light sheet microscopy. This protocol has been optimized and validated for adipose neurovascular labeling.For complete details on the use and execution of this protocol, please refer to Willows et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721008157MetabolismMicroscopyModel OrganismsNeuroscience |
| spellingShingle | Jake W. Willows Magdalena Blaszkiewicz Kristy L. Townsend A clearing-free protocol for imaging intact whole adipose tissue innervation in mice STAR Protocols Metabolism Microscopy Model Organisms Neuroscience |
| title | A clearing-free protocol for imaging intact whole adipose tissue innervation in mice |
| title_full | A clearing-free protocol for imaging intact whole adipose tissue innervation in mice |
| title_fullStr | A clearing-free protocol for imaging intact whole adipose tissue innervation in mice |
| title_full_unstemmed | A clearing-free protocol for imaging intact whole adipose tissue innervation in mice |
| title_short | A clearing-free protocol for imaging intact whole adipose tissue innervation in mice |
| title_sort | clearing free protocol for imaging intact whole adipose tissue innervation in mice |
| topic | Metabolism Microscopy Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721008157 |
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