Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.
BACKGROUND: While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. METHODOLOGY/PRINCI...
Main Authors: | , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2010-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2951895?pdf=render |
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author | Ândrea Ribeiro-dos-Santos André S Khayat Artur Silva Dayse O Alencar Jessé Lobato Larissa Luz Daniel G Pinheiro Leonardo Varuzza Monica Assumpção Paulo Assumpção Sidney Santos Dalila L Zanette Wilson A Silva Rommel Burbano Sylvain Darnet |
author_facet | Ândrea Ribeiro-dos-Santos André S Khayat Artur Silva Dayse O Alencar Jessé Lobato Larissa Luz Daniel G Pinheiro Leonardo Varuzza Monica Assumpção Paulo Assumpção Sidney Santos Dalila L Zanette Wilson A Silva Rommel Burbano Sylvain Darnet |
author_sort | Ândrea Ribeiro-dos-Santos |
collection | DOAJ |
description | BACKGROUND: While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. METHODOLOGY/PRINCIPAL FINDINGS: A small RNA library of stomach tissue was sequenced using high-throughput SOLiD sequencing technology. We obtained 261,274 quality reads with perfect matches to the human miRnome, and 42% of known miRNAs were identified. Digital Gene Expression profiling (DGE) was performed based on read abundance and showed that fifteen miRNAs were highly expressed in gastric tissue. Subsequently, the expression of these miRNAs was validated in 10 healthy individuals by RT-PCR showed a significant correlation of 83.97% (P<0.05). Six miRNAs showed a low variable pattern of expression (miR-29b, miR-29c, miR-19b, miR-31, miR-148a, miR-451) and could be considered part of the expression pattern of the healthy gastric tissue. CONCLUSIONS/SIGNIFICANCE: This study aimed to validate normal miRNA profiles of human gastric tissue to establish a reference profile for healthy individuals. Determining the regulatory processes acting in the stomach will be important in the fight against gastric cancer, which is the second-leading cause of cancer mortality worldwide. |
first_indexed | 2024-12-13T02:35:44Z |
format | Article |
id | doaj.art-a5a165a991464293b253cb78e7344d93 |
institution | Directory Open Access Journal |
issn | 1932-6203 |
language | English |
last_indexed | 2024-12-13T02:35:44Z |
publishDate | 2010-01-01 |
publisher | Public Library of Science (PLoS) |
record_format | Article |
series | PLoS ONE |
spelling | doaj.art-a5a165a991464293b253cb78e7344d932022-12-22T00:02:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-01510e1320510.1371/journal.pone.0013205Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.Ândrea Ribeiro-dos-SantosAndré S KhayatArtur SilvaDayse O AlencarJessé LobatoLarissa LuzDaniel G PinheiroLeonardo VaruzzaMonica AssumpçãoPaulo AssumpçãoSidney SantosDalila L ZanetteWilson A SilvaRommel BurbanoSylvain DarnetBACKGROUND: While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. METHODOLOGY/PRINCIPAL FINDINGS: A small RNA library of stomach tissue was sequenced using high-throughput SOLiD sequencing technology. We obtained 261,274 quality reads with perfect matches to the human miRnome, and 42% of known miRNAs were identified. Digital Gene Expression profiling (DGE) was performed based on read abundance and showed that fifteen miRNAs were highly expressed in gastric tissue. Subsequently, the expression of these miRNAs was validated in 10 healthy individuals by RT-PCR showed a significant correlation of 83.97% (P<0.05). Six miRNAs showed a low variable pattern of expression (miR-29b, miR-29c, miR-19b, miR-31, miR-148a, miR-451) and could be considered part of the expression pattern of the healthy gastric tissue. CONCLUSIONS/SIGNIFICANCE: This study aimed to validate normal miRNA profiles of human gastric tissue to establish a reference profile for healthy individuals. Determining the regulatory processes acting in the stomach will be important in the fight against gastric cancer, which is the second-leading cause of cancer mortality worldwide.http://europepmc.org/articles/PMC2951895?pdf=render |
spellingShingle | Ândrea Ribeiro-dos-Santos André S Khayat Artur Silva Dayse O Alencar Jessé Lobato Larissa Luz Daniel G Pinheiro Leonardo Varuzza Monica Assumpção Paulo Assumpção Sidney Santos Dalila L Zanette Wilson A Silva Rommel Burbano Sylvain Darnet Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach. PLoS ONE |
title | Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach. |
title_full | Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach. |
title_fullStr | Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach. |
title_full_unstemmed | Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach. |
title_short | Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach. |
title_sort | ultra deep sequencing reveals the microrna expression pattern of the human stomach |
url | http://europepmc.org/articles/PMC2951895?pdf=render |
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