Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.

BACKGROUND: While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. METHODOLOGY/PRINCI...

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Main Authors: Ândrea Ribeiro-dos-Santos, André S Khayat, Artur Silva, Dayse O Alencar, Jessé Lobato, Larissa Luz, Daniel G Pinheiro, Leonardo Varuzza, Monica Assumpção, Paulo Assumpção, Sidney Santos, Dalila L Zanette, Wilson A Silva, Rommel Burbano, Sylvain Darnet
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2951895?pdf=render
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author Ândrea Ribeiro-dos-Santos
André S Khayat
Artur Silva
Dayse O Alencar
Jessé Lobato
Larissa Luz
Daniel G Pinheiro
Leonardo Varuzza
Monica Assumpção
Paulo Assumpção
Sidney Santos
Dalila L Zanette
Wilson A Silva
Rommel Burbano
Sylvain Darnet
author_facet Ândrea Ribeiro-dos-Santos
André S Khayat
Artur Silva
Dayse O Alencar
Jessé Lobato
Larissa Luz
Daniel G Pinheiro
Leonardo Varuzza
Monica Assumpção
Paulo Assumpção
Sidney Santos
Dalila L Zanette
Wilson A Silva
Rommel Burbano
Sylvain Darnet
author_sort Ândrea Ribeiro-dos-Santos
collection DOAJ
description BACKGROUND: While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. METHODOLOGY/PRINCIPAL FINDINGS: A small RNA library of stomach tissue was sequenced using high-throughput SOLiD sequencing technology. We obtained 261,274 quality reads with perfect matches to the human miRnome, and 42% of known miRNAs were identified. Digital Gene Expression profiling (DGE) was performed based on read abundance and showed that fifteen miRNAs were highly expressed in gastric tissue. Subsequently, the expression of these miRNAs was validated in 10 healthy individuals by RT-PCR showed a significant correlation of 83.97% (P<0.05). Six miRNAs showed a low variable pattern of expression (miR-29b, miR-29c, miR-19b, miR-31, miR-148a, miR-451) and could be considered part of the expression pattern of the healthy gastric tissue. CONCLUSIONS/SIGNIFICANCE: This study aimed to validate normal miRNA profiles of human gastric tissue to establish a reference profile for healthy individuals. Determining the regulatory processes acting in the stomach will be important in the fight against gastric cancer, which is the second-leading cause of cancer mortality worldwide.
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spelling doaj.art-a5a165a991464293b253cb78e7344d932022-12-22T00:02:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-01510e1320510.1371/journal.pone.0013205Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.Ândrea Ribeiro-dos-SantosAndré S KhayatArtur SilvaDayse O AlencarJessé LobatoLarissa LuzDaniel G PinheiroLeonardo VaruzzaMonica AssumpçãoPaulo AssumpçãoSidney SantosDalila L ZanetteWilson A SilvaRommel BurbanoSylvain DarnetBACKGROUND: While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. METHODOLOGY/PRINCIPAL FINDINGS: A small RNA library of stomach tissue was sequenced using high-throughput SOLiD sequencing technology. We obtained 261,274 quality reads with perfect matches to the human miRnome, and 42% of known miRNAs were identified. Digital Gene Expression profiling (DGE) was performed based on read abundance and showed that fifteen miRNAs were highly expressed in gastric tissue. Subsequently, the expression of these miRNAs was validated in 10 healthy individuals by RT-PCR showed a significant correlation of 83.97% (P<0.05). Six miRNAs showed a low variable pattern of expression (miR-29b, miR-29c, miR-19b, miR-31, miR-148a, miR-451) and could be considered part of the expression pattern of the healthy gastric tissue. CONCLUSIONS/SIGNIFICANCE: This study aimed to validate normal miRNA profiles of human gastric tissue to establish a reference profile for healthy individuals. Determining the regulatory processes acting in the stomach will be important in the fight against gastric cancer, which is the second-leading cause of cancer mortality worldwide.http://europepmc.org/articles/PMC2951895?pdf=render
spellingShingle Ândrea Ribeiro-dos-Santos
André S Khayat
Artur Silva
Dayse O Alencar
Jessé Lobato
Larissa Luz
Daniel G Pinheiro
Leonardo Varuzza
Monica Assumpção
Paulo Assumpção
Sidney Santos
Dalila L Zanette
Wilson A Silva
Rommel Burbano
Sylvain Darnet
Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.
PLoS ONE
title Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.
title_full Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.
title_fullStr Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.
title_full_unstemmed Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.
title_short Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.
title_sort ultra deep sequencing reveals the microrna expression pattern of the human stomach
url http://europepmc.org/articles/PMC2951895?pdf=render
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