Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis.
The present study evaluated the effect of supplementing in vitro culture medium with J. insularis compared to FSH on isolated secondary follicles and in vitro maturation of oocytes from those follicles. Secondary follicles were isolated from sheep ovaries and individually cultured for 18 days in α-M...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0208760 |
| _version_ | 1818725618283446272 |
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| author | Gildas Tetaping Mbemya Jesus Cadenas Naiza Arcângela Ribeiro de Sá Denise Damasceno Guerreiro Nathalie Jiatsa Donfack Luis Alberto Vieira Francisca Geovania Canafístula de Sousa Benner Geraldo Alves Carlos Henrique Lobo Francielli Weber Santos Francisco das Chagas Lima Pinto Otília Deusdênia Loiola Pessoa Johan Smitz Pierre Comizzoli José Ricardo Figueiredo Ana Paula Ribeiro Rodrigues |
| author_facet | Gildas Tetaping Mbemya Jesus Cadenas Naiza Arcângela Ribeiro de Sá Denise Damasceno Guerreiro Nathalie Jiatsa Donfack Luis Alberto Vieira Francisca Geovania Canafístula de Sousa Benner Geraldo Alves Carlos Henrique Lobo Francielli Weber Santos Francisco das Chagas Lima Pinto Otília Deusdênia Loiola Pessoa Johan Smitz Pierre Comizzoli José Ricardo Figueiredo Ana Paula Ribeiro Rodrigues |
| author_sort | Gildas Tetaping Mbemya |
| collection | DOAJ |
| description | The present study evaluated the effect of supplementing in vitro culture medium with J. insularis compared to FSH on isolated secondary follicles and in vitro maturation of oocytes from those follicles. Secondary follicles were isolated from sheep ovaries and individually cultured for 18 days in α-MEM+ (Control), α-MEM+ supplemented with 100 ng/mL recombinant bovine follicle stimulating hormone (FSH) or with 0.3, 1.25, or 2.5 mg/mL of J. insularis extract (JI0.3, JI1.25, and JI2.5, respectively). Culture medium collected every 2 days was used to measure ROS levels. At the end of the culture period, cumulus oocytes complex (COCs) were collected and matured in vitro. Follicular walls were used for mRNA quantitation. JI0.3 led to a higher (P < 0.05) percentages of intact follicles than other groups after 18 days of culture. While follicular diameter remained unchanged from Day 6 onwards with JI0.3 and FSH, percentages of antral cavity formation were higher (P < 0.05) with JI0.3 at Day 6 than in all other treatments. No differences were observed between controls and treatment groups regarding ROS levels and mRNA expression of genes. Viability of resulting oocytes was higher (P < 0.05) in JI0.3 compared to FSH. Interestingly, in control experiment, supplementation of maturation medium with JI0.3 led to higher (P < 0.05) percentages of metaphase II compared to controls. Although more validations will be needed, it seems that this natural extract could be used as a cheap and easily available alternative to commercial FSH. |
| first_indexed | 2024-12-17T21:45:10Z |
| format | Article |
| id | doaj.art-a5a7007247df4900a902306f4e427fb6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T21:45:10Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a5a7007247df4900a902306f4e427fb62022-12-21T21:31:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011312e020876010.1371/journal.pone.0208760Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis.Gildas Tetaping MbemyaJesus CadenasNaiza Arcângela Ribeiro de SáDenise Damasceno GuerreiroNathalie Jiatsa DonfackLuis Alberto VieiraFrancisca Geovania Canafístula de SousaBenner Geraldo AlvesCarlos Henrique LoboFrancielli Weber SantosFrancisco das Chagas Lima PintoOtília Deusdênia Loiola PessoaJohan SmitzPierre ComizzoliJosé Ricardo FigueiredoAna Paula Ribeiro RodriguesThe present study evaluated the effect of supplementing in vitro culture medium with J. insularis compared to FSH on isolated secondary follicles and in vitro maturation of oocytes from those follicles. Secondary follicles were isolated from sheep ovaries and individually cultured for 18 days in α-MEM+ (Control), α-MEM+ supplemented with 100 ng/mL recombinant bovine follicle stimulating hormone (FSH) or with 0.3, 1.25, or 2.5 mg/mL of J. insularis extract (JI0.3, JI1.25, and JI2.5, respectively). Culture medium collected every 2 days was used to measure ROS levels. At the end of the culture period, cumulus oocytes complex (COCs) were collected and matured in vitro. Follicular walls were used for mRNA quantitation. JI0.3 led to a higher (P < 0.05) percentages of intact follicles than other groups after 18 days of culture. While follicular diameter remained unchanged from Day 6 onwards with JI0.3 and FSH, percentages of antral cavity formation were higher (P < 0.05) with JI0.3 at Day 6 than in all other treatments. No differences were observed between controls and treatment groups regarding ROS levels and mRNA expression of genes. Viability of resulting oocytes was higher (P < 0.05) in JI0.3 compared to FSH. Interestingly, in control experiment, supplementation of maturation medium with JI0.3 led to higher (P < 0.05) percentages of metaphase II compared to controls. Although more validations will be needed, it seems that this natural extract could be used as a cheap and easily available alternative to commercial FSH.https://doi.org/10.1371/journal.pone.0208760 |
| spellingShingle | Gildas Tetaping Mbemya Jesus Cadenas Naiza Arcângela Ribeiro de Sá Denise Damasceno Guerreiro Nathalie Jiatsa Donfack Luis Alberto Vieira Francisca Geovania Canafístula de Sousa Benner Geraldo Alves Carlos Henrique Lobo Francielli Weber Santos Francisco das Chagas Lima Pinto Otília Deusdênia Loiola Pessoa Johan Smitz Pierre Comizzoli José Ricardo Figueiredo Ana Paula Ribeiro Rodrigues Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis. PLoS ONE |
| title | Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis. |
| title_full | Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis. |
| title_fullStr | Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis. |
| title_full_unstemmed | Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis. |
| title_short | Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis. |
| title_sort | supplementation of in vitro culture medium with fsh to grow follicles and mature oocytes can be replaced by extracts of justicia insularis |
| url | https://doi.org/10.1371/journal.pone.0208760 |
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