Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells
The roles of phenolics from olive oils as effective anticancer agents have been documented in various in vitro studies of different cancer cells lines, but the relationship between the phenolic profile of olive oil and its biological activity needs more elucidation. In this study, we analysed phenol...
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MDPI AG
2020-05-01
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| Series: | Antioxidants |
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| author | Jelena Torić Anamaria Brozovic Mirela Baus Lončar Cvijeta Jakobušić Brala Ana Karković Marković Đani Benčić Monika Barbarić |
| author_facet | Jelena Torić Anamaria Brozovic Mirela Baus Lončar Cvijeta Jakobušić Brala Ana Karković Marković Đani Benčić Monika Barbarić |
| author_sort | Jelena Torić |
| collection | DOAJ |
| description | The roles of phenolics from olive oils as effective anticancer agents have been documented in various in vitro studies of different cancer cells lines, but the relationship between the phenolic profile of olive oil and its biological activity needs more elucidation. In this study, we analysed phenolic profiles of extra virgin olive oils (EVOOs) from different autochthonous cultivars from Croatia (Oblica, Bjelica, Buža, Žižolera) and investigated the biological effect of EVOO phenolic extracts (EVOO-PEs) on human cervical (HeLa) and human colon (SW48) cancer cell lines alone and in combination with cisplatin (cDDP), carboplatin (CBP), 5-fluorouracil (5-FU) and irinotecan. The quantitative evaluation of olive oil polyphenols was performed by HPLC-DAD and spectrophotometric analysis. The biological effect of EVOO-PEs alone and in combination with anticancer drugs was measured by MTT assay. Analysed EVOO-PEs differ in phenolic profile and inhibited HeLa and SW48 cells in a dose-dependent manner. Further, it is shown that EVOO-PEs (Oblica-Sea, Buža and Žižolera), in combination with anticancer drugs, increase the metabolic activity of HeLa and SW48 cells and have a protective role. These data imply careful consummation of olive oil during chemotherapy of cancer patients. |
| first_indexed | 2024-03-10T19:37:55Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T19:37:55Z |
| publishDate | 2020-05-01 |
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| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a5a84e40f0aa4b36911c5f716b672a2f2023-11-20T01:33:48ZengMDPI AGAntioxidants2076-39212020-05-019545310.3390/antiox9050453Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma CellsJelena Torić0Anamaria Brozovic1Mirela Baus Lončar2Cvijeta Jakobušić Brala3Ana Karković Marković4Đani Benčić5Monika Barbarić6Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, Zagreb 10000, CroatiaRuđer Bošković Institute, Bijenička Cesta 54, Zagreb 10000, CroatiaRuđer Bošković Institute, Bijenička Cesta 54, Zagreb 10000, CroatiaFaculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, Zagreb 10000, CroatiaFaculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, Zagreb 10000, CroatiaFaculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, Zagreb 10000, CroatiaFaculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, Zagreb 10000, CroatiaThe roles of phenolics from olive oils as effective anticancer agents have been documented in various in vitro studies of different cancer cells lines, but the relationship between the phenolic profile of olive oil and its biological activity needs more elucidation. In this study, we analysed phenolic profiles of extra virgin olive oils (EVOOs) from different autochthonous cultivars from Croatia (Oblica, Bjelica, Buža, Žižolera) and investigated the biological effect of EVOO phenolic extracts (EVOO-PEs) on human cervical (HeLa) and human colon (SW48) cancer cell lines alone and in combination with cisplatin (cDDP), carboplatin (CBP), 5-fluorouracil (5-FU) and irinotecan. The quantitative evaluation of olive oil polyphenols was performed by HPLC-DAD and spectrophotometric analysis. The biological effect of EVOO-PEs alone and in combination with anticancer drugs was measured by MTT assay. Analysed EVOO-PEs differ in phenolic profile and inhibited HeLa and SW48 cells in a dose-dependent manner. Further, it is shown that EVOO-PEs (Oblica-Sea, Buža and Žižolera), in combination with anticancer drugs, increase the metabolic activity of HeLa and SW48 cells and have a protective role. These data imply careful consummation of olive oil during chemotherapy of cancer patients.https://www.mdpi.com/2076-3921/9/5/453olive oilphenolicsbiological activitycancer cellsanticancer drugs |
| spellingShingle | Jelena Torić Anamaria Brozovic Mirela Baus Lončar Cvijeta Jakobušić Brala Ana Karković Marković Đani Benčić Monika Barbarić Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells Antioxidants olive oil phenolics biological activity cancer cells anticancer drugs |
| title | Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells |
| title_full | Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells |
| title_fullStr | Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells |
| title_full_unstemmed | Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells |
| title_short | Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells |
| title_sort | biological activity of phenolic compounds in extra virgin olive oils through their phenolic profile and their combination with anticancer drugs observed in human cervical carcinoma and colon adenocarcinoma cells |
| topic | olive oil phenolics biological activity cancer cells anticancer drugs |
| url | https://www.mdpi.com/2076-3921/9/5/453 |
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