Shift in the Dominant Sequence Type of Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infection from ST11 to ST15 at a Medical Center in Northeast China, 2015–2020
Jingjing Chen,1– 3 Chang Hu,1– 3 Ruixuan Wang,1– 3 Fushun Li,1– 3 Guoquan Sun,1– 3 Min Yang,1– 3 Yunzhuo Chu1– 3 1Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, People&r...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-05-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/shift-in-the-dominant-sequence-type-of-carbapenem-resistant-klebsiella-peer-reviewed-fulltext-article-IDR |
| _version_ | 1818599833738412032 |
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| author | Chen J Hu C Wang R Li F Sun G Yang M Chu Y |
| author_facet | Chen J Hu C Wang R Li F Sun G Yang M Chu Y |
| author_sort | Chen J |
| collection | DOAJ |
| description | Jingjing Chen,1– 3 Chang Hu,1– 3 Ruixuan Wang,1– 3 Fushun Li,1– 3 Guoquan Sun,1– 3 Min Yang,1– 3 Yunzhuo Chu1– 3 1Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, People’s Republic of China; 2National Clinical Research Center for Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, People’s Republic of China; 3Labortory Medicine Innovation Unit, Chinese Academy of Medical Sciences, Shenyang, People’s Republic of ChinaCorrespondence: Yunzhuo ChuDepartment of Laboratory Medicine, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang, Liaoning, People’s Republic of ChinaTel/Fax +862483282634Email cyz6630@163.comObjective: To investigate the clinical characteristics and molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae (CRKP) bloodstream infection at a medical center in northeast China, especially after coronavirus disease (COVID-19) pandemic.Methods: Fifty-one patients were diagnosed with CRKP bloodstream infection between January 2015 and December 2020, among which 42 isolates were available for further study. Species identification and antibiotic susceptibilities were tested with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and VITEK 2 systems. Carbapenemase genes, virulence genes and MLST genes were detected by polymerase chain reaction. Moreover, the string test and serum killing assay were performed to evaluate the virulence of the CRKP isolates.Results: During the six-year period, the detection rate of CRKP in bloodstream infection showed an increasing trend, with the intensive care unit, hematology and respiratory medicine wards mainly affected. Molecular epidemiology analyses showed that KPC-2 was the dominant carbapenemase gene. In addition, the dominant sequence type (ST) of CRKP shifted from ST11 to ST15 strains, which were all sensitive to amikacin in contrast to the ST11 stains. Furthermore, ST15 CRKP strains were positive for the KfuB virulence gene and more resistant to serum killing compared to the ST11 CRKP strains. Nonetheless, the mortality rate of patients infected with ST11 and ST15 CRKP did not show any significant differences.Conclusion: A shift in the dominant sequence type of CRKP bloodstream infections from ST11 to ST15 was observed during the years 2015– 2020. Compared to ST11, the ST15 CRKP strains showed amikacin sensitivity, positivity for KfuB gene, and serum resistance, which may indicate stronger virulence.Keywords: carbapenem-resistant Klebsiella pneumoniae, sequence type, KPC-2, virulence, drug resistance |
| first_indexed | 2024-12-16T12:25:53Z |
| format | Article |
| id | doaj.art-a5abd4470fd54e508c67246281cd2e6e |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-16T12:25:53Z |
| publishDate | 2021-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-a5abd4470fd54e508c67246281cd2e6e2022-12-21T22:31:50ZengDove Medical PressInfection and Drug Resistance1178-69732021-05-01Volume 141855186365036Shift in the Dominant Sequence Type of Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infection from ST11 to ST15 at a Medical Center in Northeast China, 2015–2020Chen JHu CWang RLi FSun GYang MChu YJingjing Chen,1– 3 Chang Hu,1– 3 Ruixuan Wang,1– 3 Fushun Li,1– 3 Guoquan Sun,1– 3 Min Yang,1– 3 Yunzhuo Chu1– 3 1Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, People’s Republic of China; 2National Clinical Research Center for Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, People’s Republic of China; 3Labortory Medicine Innovation Unit, Chinese Academy of Medical Sciences, Shenyang, People’s Republic of ChinaCorrespondence: Yunzhuo ChuDepartment of Laboratory Medicine, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang, Liaoning, People’s Republic of ChinaTel/Fax +862483282634Email cyz6630@163.comObjective: To investigate the clinical characteristics and molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae (CRKP) bloodstream infection at a medical center in northeast China, especially after coronavirus disease (COVID-19) pandemic.Methods: Fifty-one patients were diagnosed with CRKP bloodstream infection between January 2015 and December 2020, among which 42 isolates were available for further study. Species identification and antibiotic susceptibilities were tested with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and VITEK 2 systems. Carbapenemase genes, virulence genes and MLST genes were detected by polymerase chain reaction. Moreover, the string test and serum killing assay were performed to evaluate the virulence of the CRKP isolates.Results: During the six-year period, the detection rate of CRKP in bloodstream infection showed an increasing trend, with the intensive care unit, hematology and respiratory medicine wards mainly affected. Molecular epidemiology analyses showed that KPC-2 was the dominant carbapenemase gene. In addition, the dominant sequence type (ST) of CRKP shifted from ST11 to ST15 strains, which were all sensitive to amikacin in contrast to the ST11 stains. Furthermore, ST15 CRKP strains were positive for the KfuB virulence gene and more resistant to serum killing compared to the ST11 CRKP strains. Nonetheless, the mortality rate of patients infected with ST11 and ST15 CRKP did not show any significant differences.Conclusion: A shift in the dominant sequence type of CRKP bloodstream infections from ST11 to ST15 was observed during the years 2015– 2020. Compared to ST11, the ST15 CRKP strains showed amikacin sensitivity, positivity for KfuB gene, and serum resistance, which may indicate stronger virulence.Keywords: carbapenem-resistant Klebsiella pneumoniae, sequence type, KPC-2, virulence, drug resistancehttps://www.dovepress.com/shift-in-the-dominant-sequence-type-of-carbapenem-resistant-klebsiella-peer-reviewed-fulltext-article-IDRcarbapenem-resistant klebsiella pneumoniaesequence typekpc-2virulencedrug resistance |
| spellingShingle | Chen J Hu C Wang R Li F Sun G Yang M Chu Y Shift in the Dominant Sequence Type of Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infection from ST11 to ST15 at a Medical Center in Northeast China, 2015–2020 Infection and Drug Resistance carbapenem-resistant klebsiella pneumoniae sequence type kpc-2 virulence drug resistance |
| title | Shift in the Dominant Sequence Type of Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infection from ST11 to ST15 at a Medical Center in Northeast China, 2015–2020 |
| title_full | Shift in the Dominant Sequence Type of Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infection from ST11 to ST15 at a Medical Center in Northeast China, 2015–2020 |
| title_fullStr | Shift in the Dominant Sequence Type of Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infection from ST11 to ST15 at a Medical Center in Northeast China, 2015–2020 |
| title_full_unstemmed | Shift in the Dominant Sequence Type of Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infection from ST11 to ST15 at a Medical Center in Northeast China, 2015–2020 |
| title_short | Shift in the Dominant Sequence Type of Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infection from ST11 to ST15 at a Medical Center in Northeast China, 2015–2020 |
| title_sort | shift in the dominant sequence type of carbapenem resistant klebsiella pneumoniae bloodstream infection from st11 to st15 at a medical center in northeast china 2015 ndash 2020 |
| topic | carbapenem-resistant klebsiella pneumoniae sequence type kpc-2 virulence drug resistance |
| url | https://www.dovepress.com/shift-in-the-dominant-sequence-type-of-carbapenem-resistant-klebsiella-peer-reviewed-fulltext-article-IDR |
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