Mice Generated with Induced Pluripotent Stem Cells Derived from Mucosal-Associated Invariant T Cells
The function of mucosal-associated invariant T (MAIT) cells, a burgeoning member of innate-like T cells abundant in humans and implicated in many diseases, remains obscure. To explore this, mice with a rearranged T cell receptor (TCR) α or β locus, specific for MAIT cells, were generated via induced...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/1/137 |
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| author | Chie Sugimoto Hiroyoshi Fujita Hiroshi Wakao |
| author_facet | Chie Sugimoto Hiroyoshi Fujita Hiroshi Wakao |
| author_sort | Chie Sugimoto |
| collection | DOAJ |
| description | The function of mucosal-associated invariant T (MAIT) cells, a burgeoning member of innate-like T cells abundant in humans and implicated in many diseases, remains obscure. To explore this, mice with a rearranged T cell receptor (TCR) α or β locus, specific for MAIT cells, were generated via induced pluripotent stem cells derived from MAIT cells and were designated Vα19 and Vβ8 mice, respectively. Both groups of mice expressed large numbers of MAIT cells. The MAIT cells from these mice were activated by cytokines and an agonist to produce IFN-γ and IL-17. While Vβ8 mice showed resistance in a cancer metastasis model, Vα19 mice did not. Adoptive transfer of MAIT cells from the latter into the control mice, however, recapitulated the resistance. These mice present an implication for understanding the role of MAIT cells in health and disease and in developing treatments for the plethora of diseases in which MAIT cells are implicated. |
| first_indexed | 2024-03-08T09:57:03Z |
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| id | doaj.art-a5ae290c12bc4c4a97b19fa95879b962 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-08T09:57:03Z |
| publishDate | 2024-01-01 |
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| series | Biomedicines |
| spelling | doaj.art-a5ae290c12bc4c4a97b19fa95879b9622024-01-29T13:47:27ZengMDPI AGBiomedicines2227-90592024-01-0112113710.3390/biomedicines12010137Mice Generated with Induced Pluripotent Stem Cells Derived from Mucosal-Associated Invariant T CellsChie Sugimoto0Hiroyoshi Fujita1Hiroshi Wakao2Host Defense Division, Research Centre for Advanced Medical Science, Dokkyo Medical University, Mibu 321-0293, JapanHost Defense Division, Research Centre for Advanced Medical Science, Dokkyo Medical University, Mibu 321-0293, JapanHost Defense Division, Research Centre for Advanced Medical Science, Dokkyo Medical University, Mibu 321-0293, JapanThe function of mucosal-associated invariant T (MAIT) cells, a burgeoning member of innate-like T cells abundant in humans and implicated in many diseases, remains obscure. To explore this, mice with a rearranged T cell receptor (TCR) α or β locus, specific for MAIT cells, were generated via induced pluripotent stem cells derived from MAIT cells and were designated Vα19 and Vβ8 mice, respectively. Both groups of mice expressed large numbers of MAIT cells. The MAIT cells from these mice were activated by cytokines and an agonist to produce IFN-γ and IL-17. While Vβ8 mice showed resistance in a cancer metastasis model, Vα19 mice did not. Adoptive transfer of MAIT cells from the latter into the control mice, however, recapitulated the resistance. These mice present an implication for understanding the role of MAIT cells in health and disease and in developing treatments for the plethora of diseases in which MAIT cells are implicated.https://www.mdpi.com/2227-9059/12/1/137MAIT cellsiPS cellschimeric micerearranged TCRanti-metastatic activity |
| spellingShingle | Chie Sugimoto Hiroyoshi Fujita Hiroshi Wakao Mice Generated with Induced Pluripotent Stem Cells Derived from Mucosal-Associated Invariant T Cells Biomedicines MAIT cells iPS cells chimeric mice rearranged TCR anti-metastatic activity |
| title | Mice Generated with Induced Pluripotent Stem Cells Derived from Mucosal-Associated Invariant T Cells |
| title_full | Mice Generated with Induced Pluripotent Stem Cells Derived from Mucosal-Associated Invariant T Cells |
| title_fullStr | Mice Generated with Induced Pluripotent Stem Cells Derived from Mucosal-Associated Invariant T Cells |
| title_full_unstemmed | Mice Generated with Induced Pluripotent Stem Cells Derived from Mucosal-Associated Invariant T Cells |
| title_short | Mice Generated with Induced Pluripotent Stem Cells Derived from Mucosal-Associated Invariant T Cells |
| title_sort | mice generated with induced pluripotent stem cells derived from mucosal associated invariant t cells |
| topic | MAIT cells iPS cells chimeric mice rearranged TCR anti-metastatic activity |
| url | https://www.mdpi.com/2227-9059/12/1/137 |
| work_keys_str_mv | AT chiesugimoto micegeneratedwithinducedpluripotentstemcellsderivedfrommucosalassociatedinvarianttcells AT hiroyoshifujita micegeneratedwithinducedpluripotentstemcellsderivedfrommucosalassociatedinvarianttcells AT hiroshiwakao micegeneratedwithinducedpluripotentstemcellsderivedfrommucosalassociatedinvarianttcells |