Mechanism of lead adsorption by a Bacillus cereus strain with indole-3-acetic acid secretion and inorganic phosphorus dissolution functions
Abstract Background Heavy metal pollution has become a major source of environmental pollution because of increasing industrialization. Microbial remediation is a promising approach to remediate lead-contaminated environments owing to its cost-effective, environment-friendly, ecologically sustainabl...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-023-02795-z |
| _version_ | 1797865425215684608 |
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| author | Qingrong Li Wenbo Zhang Sentai Liao Dongxu Xing Yang Xiao Donglai Zhou Qiong Yang |
| author_facet | Qingrong Li Wenbo Zhang Sentai Liao Dongxu Xing Yang Xiao Donglai Zhou Qiong Yang |
| author_sort | Qingrong Li |
| collection | DOAJ |
| description | Abstract Background Heavy metal pollution has become a major source of environmental pollution because of increasing industrialization. Microbial remediation is a promising approach to remediate lead-contaminated environments owing to its cost-effective, environment-friendly, ecologically sustainable, and highly efficient properties. In this study, the growth-promoting functions and lead-adsorption ability of Bacillus cereus SEM-15 were examined, and the functional mechanism of the strain was preliminarily identified using scanning electron microscopy, energy spectrum, infrared spectrum, and genome analyses, providing theoretical support for utilization of B. cereus SEM-15 in heavy metals remediation. Results B. cereus SEM-15 showed strong ability to dissolve inorganic phosphorus and secrete indole-3-acetic acid. The lead adsorption efficiency of the strain at lead ion concentration of 150 mg/L was more than 93%. Single factor analysis revealed the optimal conditions for heavy metal adsorption by B. cereus SEM-15 (adsorption time, initial lead ion concentration, pH, and inoculum amount were 10 min, 50–150 mg/L, 6–7, and 5 g/L, respectively) in nutrient-free environment, with the lead adsorption rate reaching 96.58%. Scanning electron microscopy of B. cereus SEM-15 cells before and after lead adsorption showed adherence of a large number of granular precipitates to the cell surface after lead adsorption. X-Ray photoelectron spectroscopy and Fourier transform infrared spectroscopy results indicated the characteristic peaks of Pb–O, Pb–O-R (R = functional group), and Pb–S bonds after lead adsorption, and a shift in the characteristic peaks of bonds and groups related to C, N, and O. Genome annotation results showed the presence of genes related to heavy metals tolerance and plant growth promotion in B. cereus SEM-15, providing a molecular basis for the strain’s heavy metals tolerance and plant growth promotion functions. Conclusions This study analyzed the lead adsorption characteristics of B. cereus SEM-15 and the associated influencing factors, and discussed the adsorption mechanism and related functional genes, providing a basis for clarifying the underlying molecular mechanism and offering a reference for further research on plant-microorganisms combined remediation of heavy metals polluted environments. |
| first_indexed | 2024-04-09T23:08:37Z |
| format | Article |
| id | doaj.art-a5ae770d4048427fa179eea2de9ac9f9 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-09T23:08:37Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-a5ae770d4048427fa179eea2de9ac9f92023-03-22T10:34:18ZengBMCBMC Microbiology1471-21802023-03-0123111410.1186/s12866-023-02795-zMechanism of lead adsorption by a Bacillus cereus strain with indole-3-acetic acid secretion and inorganic phosphorus dissolution functionsQingrong Li0Wenbo Zhang1Sentai Liao2Dongxu Xing3Yang Xiao4Donglai Zhou5Qiong Yang6Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural SciencesSericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural SciencesSericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural SciencesSericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural SciencesSericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural SciencesSericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural SciencesSericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural SciencesAbstract Background Heavy metal pollution has become a major source of environmental pollution because of increasing industrialization. Microbial remediation is a promising approach to remediate lead-contaminated environments owing to its cost-effective, environment-friendly, ecologically sustainable, and highly efficient properties. In this study, the growth-promoting functions and lead-adsorption ability of Bacillus cereus SEM-15 were examined, and the functional mechanism of the strain was preliminarily identified using scanning electron microscopy, energy spectrum, infrared spectrum, and genome analyses, providing theoretical support for utilization of B. cereus SEM-15 in heavy metals remediation. Results B. cereus SEM-15 showed strong ability to dissolve inorganic phosphorus and secrete indole-3-acetic acid. The lead adsorption efficiency of the strain at lead ion concentration of 150 mg/L was more than 93%. Single factor analysis revealed the optimal conditions for heavy metal adsorption by B. cereus SEM-15 (adsorption time, initial lead ion concentration, pH, and inoculum amount were 10 min, 50–150 mg/L, 6–7, and 5 g/L, respectively) in nutrient-free environment, with the lead adsorption rate reaching 96.58%. Scanning electron microscopy of B. cereus SEM-15 cells before and after lead adsorption showed adherence of a large number of granular precipitates to the cell surface after lead adsorption. X-Ray photoelectron spectroscopy and Fourier transform infrared spectroscopy results indicated the characteristic peaks of Pb–O, Pb–O-R (R = functional group), and Pb–S bonds after lead adsorption, and a shift in the characteristic peaks of bonds and groups related to C, N, and O. Genome annotation results showed the presence of genes related to heavy metals tolerance and plant growth promotion in B. cereus SEM-15, providing a molecular basis for the strain’s heavy metals tolerance and plant growth promotion functions. Conclusions This study analyzed the lead adsorption characteristics of B. cereus SEM-15 and the associated influencing factors, and discussed the adsorption mechanism and related functional genes, providing a basis for clarifying the underlying molecular mechanism and offering a reference for further research on plant-microorganisms combined remediation of heavy metals polluted environments.https://doi.org/10.1186/s12866-023-02795-zPlant growth promotingHeavy metalLeadMicrobial remediation |
| spellingShingle | Qingrong Li Wenbo Zhang Sentai Liao Dongxu Xing Yang Xiao Donglai Zhou Qiong Yang Mechanism of lead adsorption by a Bacillus cereus strain with indole-3-acetic acid secretion and inorganic phosphorus dissolution functions BMC Microbiology Plant growth promoting Heavy metal Lead Microbial remediation |
| title | Mechanism of lead adsorption by a Bacillus cereus strain with indole-3-acetic acid secretion and inorganic phosphorus dissolution functions |
| title_full | Mechanism of lead adsorption by a Bacillus cereus strain with indole-3-acetic acid secretion and inorganic phosphorus dissolution functions |
| title_fullStr | Mechanism of lead adsorption by a Bacillus cereus strain with indole-3-acetic acid secretion and inorganic phosphorus dissolution functions |
| title_full_unstemmed | Mechanism of lead adsorption by a Bacillus cereus strain with indole-3-acetic acid secretion and inorganic phosphorus dissolution functions |
| title_short | Mechanism of lead adsorption by a Bacillus cereus strain with indole-3-acetic acid secretion and inorganic phosphorus dissolution functions |
| title_sort | mechanism of lead adsorption by a bacillus cereus strain with indole 3 acetic acid secretion and inorganic phosphorus dissolution functions |
| topic | Plant growth promoting Heavy metal Lead Microbial remediation |
| url | https://doi.org/10.1186/s12866-023-02795-z |
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