Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect
Background: The Mentha-Pulegium essential oil (MP-EO) contains different antioxidant compounds and reduces the indirect effects of dispersed ionizing radiation on biological systems. Objective: The current study aimed to assess a possible radio-protective effect of MP-EO on peripheral blood mononucl...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2022-04-01
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| Series: | Journal of Biomedical Physics and Engineering |
| Subjects: | |
| Online Access: | https://jbpe.sums.ac.ir/article_48202_d3e4689b77148c28748580642656e728.pdf |
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| author | Nima Hamzian Abolfazl Nickfarjam Ali Shams Fateme Haghiralsadat Moslem Najmi-Nezhad |
| author_facet | Nima Hamzian Abolfazl Nickfarjam Ali Shams Fateme Haghiralsadat Moslem Najmi-Nezhad |
| author_sort | Nima Hamzian |
| collection | DOAJ |
| description | Background: The Mentha-Pulegium essential oil (MP-EO) contains different antioxidant compounds and reduces the indirect effects of dispersed ionizing radiation on biological systems. Objective: The current study aimed to assess a possible radio-protective effect of MP-EO on peripheral blood mononuclear cells (PBMCs).Material and Methods: In this experimental study, MP-EO was firstly prepared and PBMCs were then irradiated in various groups with doses of 25 and 200 cGy of X-rays in the presence of IC10 of MP-EO. After incubation times of 48h and 72h, the survival, apoptosis, and necrosis percentages of PBMCs were determined by MTT assay and flow cytometry analyses; the radio-protective effect of MP-EO was examined. Results: In the presence of 80 µg/ml (IC10) MP-EO, the mean survival percentage of irradiated PBMCs by radiation doses of 25 and 200 cGy was significantly increased after 48h of incubation compared with the control. At 72h of incubation, the mean survival percentage of irradiated PBMCs was significantly increased only at 25 cGy. The percentage of apoptosis and necrosis of PBMCs was significantly reduced in the presence of the MP-EO at both incubation times and radiation doses; therefore, the highest reduction was at 200 cGy and 48h incubation compared to the control. Conclusion: MP-EO as a natural, non-toxic, and cost-effective compound can exhibit a favorable in-vitro radio-protective effect by increasing the survival and decreasing the percentage of apoptosis and necrosis of irradiated PBMCs. |
| first_indexed | 2024-04-14T01:04:39Z |
| format | Article |
| id | doaj.art-a5b2fe5e27b348cda073f6c97dfea270 |
| institution | Directory Open Access Journal |
| issn | 2251-7200 |
| language | English |
| last_indexed | 2024-04-14T01:04:39Z |
| publishDate | 2022-04-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Biomedical Physics and Engineering |
| spelling | doaj.art-a5b2fe5e27b348cda073f6c97dfea2702022-12-22T02:21:18ZengShiraz University of Medical SciencesJournal of Biomedical Physics and Engineering2251-72002022-04-0112213714810.31661/jbpe.v0i0.2109-139748202Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective EffectNima Hamzian0Abolfazl Nickfarjam1Ali Shams2Fateme Haghiralsadat3Moslem Najmi-Nezhad4PhD, Department of Medical Physics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranPhD, Department of Medical Physics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranPhD, Department of Immunology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranPhD, Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranMSc, Department of Medical Physics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranBackground: The Mentha-Pulegium essential oil (MP-EO) contains different antioxidant compounds and reduces the indirect effects of dispersed ionizing radiation on biological systems. Objective: The current study aimed to assess a possible radio-protective effect of MP-EO on peripheral blood mononuclear cells (PBMCs).Material and Methods: In this experimental study, MP-EO was firstly prepared and PBMCs were then irradiated in various groups with doses of 25 and 200 cGy of X-rays in the presence of IC10 of MP-EO. After incubation times of 48h and 72h, the survival, apoptosis, and necrosis percentages of PBMCs were determined by MTT assay and flow cytometry analyses; the radio-protective effect of MP-EO was examined. Results: In the presence of 80 µg/ml (IC10) MP-EO, the mean survival percentage of irradiated PBMCs by radiation doses of 25 and 200 cGy was significantly increased after 48h of incubation compared with the control. At 72h of incubation, the mean survival percentage of irradiated PBMCs was significantly increased only at 25 cGy. The percentage of apoptosis and necrosis of PBMCs was significantly reduced in the presence of the MP-EO at both incubation times and radiation doses; therefore, the highest reduction was at 200 cGy and 48h incubation compared to the control. Conclusion: MP-EO as a natural, non-toxic, and cost-effective compound can exhibit a favorable in-vitro radio-protective effect by increasing the survival and decreasing the percentage of apoptosis and necrosis of irradiated PBMCs.https://jbpe.sums.ac.ir/article_48202_d3e4689b77148c28748580642656e728.pdflymphocytesmentha pulegium essential oilradiation, ionizingradioprotectiveapoptosisnecrosis |
| spellingShingle | Nima Hamzian Abolfazl Nickfarjam Ali Shams Fateme Haghiralsadat Moslem Najmi-Nezhad Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect Journal of Biomedical Physics and Engineering lymphocytes mentha pulegium essential oil radiation, ionizing radioprotective apoptosis necrosis |
| title | Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect |
| title_full | Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect |
| title_fullStr | Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect |
| title_full_unstemmed | Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect |
| title_short | Effects of Ionizing Radiation on Human Peripheral Blood Mononuclear Cells (PBMCs) in the Presence of Mentha-Pulegium Essential Oil: A Study on the Radioprotective Effect |
| title_sort | effects of ionizing radiation on human peripheral blood mononuclear cells pbmcs in the presence of mentha pulegium essential oil a study on the radioprotective effect |
| topic | lymphocytes mentha pulegium essential oil radiation, ionizing radioprotective apoptosis necrosis |
| url | https://jbpe.sums.ac.ir/article_48202_d3e4689b77148c28748580642656e728.pdf |
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