Transcriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapers
Abstract Rationale Electronic (e)-cigarettes are popular among youth and cigarette smokers attempting to quit. Studies to date have focused on the utility of e-cigarettes as a smoking cessation tool, but the biological effects are largely unknown. Objectives To identify transcriptomic differences in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12931-023-02438-x |
| _version_ | 1797822754069676032 |
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| author | Mario F. Perez Marina Yurieva Spandana Poddutoori Eric M. Mortensen Laura E. Crotty Alexander Adam Williams |
| author_facet | Mario F. Perez Marina Yurieva Spandana Poddutoori Eric M. Mortensen Laura E. Crotty Alexander Adam Williams |
| author_sort | Mario F. Perez |
| collection | DOAJ |
| description | Abstract Rationale Electronic (e)-cigarettes are popular among youth and cigarette smokers attempting to quit. Studies to date have focused on the utility of e-cigarettes as a smoking cessation tool, but the biological effects are largely unknown. Objectives To identify transcriptomic differences in the blood and sputum of e-cigarette users compared to conventional cigarettes smokers and healthy controls and describe biological pathways affected by these tobacco products. Methods Cross-sectional analysis of whole blood and sputum RNA-sequencing data from 8 smokers, 9 e-cigarette users (e-cigs) and 4 controls. Weighted gene co-network analysis (WGCNA) identified gene module associations. Ingenuity Pathway Analysis (IPA) identified canonical pathways associated with tobacco products. Main results In blood, a three-group comparison showed 16 differentially expressed genes (DEGs); pair-wise comparison showed 7 DEGs between e-cigs and controls, 35 DEGs between smokers and controls, and 13 DEGs between smokers and e-cigs. In sputum, 438 DEGs were in the three-group comparison. In pair-wise comparisons, there were 2 DEGs between e-cigs and controls, 270 DEGs between smokers and controls, and 468 DEGs between smokers and e-cigs. Only 2 genes in the smokers vs. control comparison overlapped between blood and sputum. Most gene modules identified through WGCNA associated with tobacco product exposures also were associated with cotinine and exhaled CO levels. IPA showed more canonical pathways altered by conventional cigarette smoking than by e-cigarette use. Conclusion Cigarette smoking and e-cigarette use led to transcriptomic changes in both blood and sputum. However, conventional cigarettes induced much stronger transcriptomic responses in both compartments. |
| first_indexed | 2024-03-13T10:12:47Z |
| format | Article |
| id | doaj.art-a5b311460bd240d2a7bd9d5ef7fed504 |
| institution | Directory Open Access Journal |
| issn | 1465-993X |
| language | English |
| last_indexed | 2024-03-13T10:12:47Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-a5b311460bd240d2a7bd9d5ef7fed5042023-05-21T11:24:08ZengBMCRespiratory Research1465-993X2023-05-0124111310.1186/s12931-023-02438-xTranscriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapersMario F. Perez0Marina Yurieva1Spandana Poddutoori2Eric M. Mortensen3Laura E. Crotty Alexander4Adam Williams5Department of Pulmonary, Critical Care and Sleep Medicine, University of Connecticut School of MedicineThe Jackson Laboratory for Genomic MedicineAXA Life and Health Re US, Inc.Department of Pulmonary, Critical Care and Sleep Medicine, University of Connecticut School of MedicineDivision of Pulmonary Critical Care, Department of Medicine, University of California San DiegoThe Jackson Laboratory for Genomic MedicineAbstract Rationale Electronic (e)-cigarettes are popular among youth and cigarette smokers attempting to quit. Studies to date have focused on the utility of e-cigarettes as a smoking cessation tool, but the biological effects are largely unknown. Objectives To identify transcriptomic differences in the blood and sputum of e-cigarette users compared to conventional cigarettes smokers and healthy controls and describe biological pathways affected by these tobacco products. Methods Cross-sectional analysis of whole blood and sputum RNA-sequencing data from 8 smokers, 9 e-cigarette users (e-cigs) and 4 controls. Weighted gene co-network analysis (WGCNA) identified gene module associations. Ingenuity Pathway Analysis (IPA) identified canonical pathways associated with tobacco products. Main results In blood, a three-group comparison showed 16 differentially expressed genes (DEGs); pair-wise comparison showed 7 DEGs between e-cigs and controls, 35 DEGs between smokers and controls, and 13 DEGs between smokers and e-cigs. In sputum, 438 DEGs were in the three-group comparison. In pair-wise comparisons, there were 2 DEGs between e-cigs and controls, 270 DEGs between smokers and controls, and 468 DEGs between smokers and e-cigs. Only 2 genes in the smokers vs. control comparison overlapped between blood and sputum. Most gene modules identified through WGCNA associated with tobacco product exposures also were associated with cotinine and exhaled CO levels. IPA showed more canonical pathways altered by conventional cigarette smoking than by e-cigarette use. Conclusion Cigarette smoking and e-cigarette use led to transcriptomic changes in both blood and sputum. However, conventional cigarettes induced much stronger transcriptomic responses in both compartments.https://doi.org/10.1186/s12931-023-02438-xTranscriptomicsTobaccoImmune cells of the airwaysElectronic cigarettesCigarette smoking |
| spellingShingle | Mario F. Perez Marina Yurieva Spandana Poddutoori Eric M. Mortensen Laura E. Crotty Alexander Adam Williams Transcriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapers Respiratory Research Transcriptomics Tobacco Immune cells of the airways Electronic cigarettes Cigarette smoking |
| title | Transcriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapers |
| title_full | Transcriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapers |
| title_fullStr | Transcriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapers |
| title_full_unstemmed | Transcriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapers |
| title_short | Transcriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapers |
| title_sort | transcriptomic responses in the blood and sputum of cigarette smokers compared to e cigarette vapers |
| topic | Transcriptomics Tobacco Immune cells of the airways Electronic cigarettes Cigarette smoking |
| url | https://doi.org/10.1186/s12931-023-02438-x |
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