Long Non-coding RNA RP11-480I12.5 Promotes the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-490-3p-AURKA-Wnt/β-Catenin Axis
Background: RP11-480I12. 5 is a newly identified long non-coding RNA (lncRNA) that has never been studied in breast cancer (BC). The biological function of RP11-480I12.5 in breast carcinoma and its underlying mechanism are still unknown.Methods: We scanned The Cancer Genome Atlas (TCGA) database and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2020.00948/full |
| _version_ | 1818228872024424448 |
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| author | Xinya Gao Yuanhui Lai Zhanqiang Zhang Yanfei Ma Zhizhai Luo Yanghong Li Ciqiu Yang Guanming Lu Jie Li |
| author_facet | Xinya Gao Yuanhui Lai Zhanqiang Zhang Yanfei Ma Zhizhai Luo Yanghong Li Ciqiu Yang Guanming Lu Jie Li |
| author_sort | Xinya Gao |
| collection | DOAJ |
| description | Background: RP11-480I12. 5 is a newly identified long non-coding RNA (lncRNA) that has never been studied in breast cancer (BC). The biological function of RP11-480I12.5 in breast carcinoma and its underlying mechanism are still unknown.Methods: We scanned The Cancer Genome Atlas (TCGA) database and identified RP11-480I12.5 as one of the most dysregulated lncRNAs. The level of RP11-480I12.5 was assessed in BC tissue samples and BC cell lines. The prognostic value of RP11-480I12.5 expression was assessed using the Kaplan–Meier method. The biological influence of RP11-480I12.5 on BC cell lines was studied using proliferation and Transwell migration and invasion assays.Results: RP11-480I12.5 expression was upregulated in data from both the TCGA database and our own database. Moreover, Kaplan–Meier and Cox proportional hazard analyses indicated that high RP11-480I12.5 expression was related to poor overall survival. Moreover, RP11-480I12.5 promoted the proliferation, migration, and invasion of BC. RP11-480I12.5 promoted the expression of AURKA and the activation of the downstream Wnt/β-catenin pathway by sponging the microRNA (miRNA) miR-490-3p.Conclusion: Taken together, our results indicate that RP11-480I12.5 is associated with tumor progression in BCs. Our findings indicate that the lncRNA RP11-480I12.5 promotes the proliferation, migration, and invasion of BC cells through the miR-490-3p-AURKA-Wnt/β-catenin axis, which may serve as a therapeutic target in the future. |
| first_indexed | 2024-12-12T10:09:36Z |
| format | Article |
| id | doaj.art-a5b5ba62365a47f0aa4e82ee626b46a3 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-12T10:09:36Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-a5b5ba62365a47f0aa4e82ee626b46a32022-12-22T00:27:50ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-07-011010.3389/fonc.2020.00948536224Long Non-coding RNA RP11-480I12.5 Promotes the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-490-3p-AURKA-Wnt/β-Catenin AxisXinya Gao0Yuanhui Lai1Zhanqiang Zhang2Yanfei Ma3Zhizhai Luo4Yanghong Li5Ciqiu Yang6Guanming Lu7Jie Li8Department of Neurosurgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Breast and Thyroid Surgery, Eastern Hospital of the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Breast and Thyroid Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Breast and Thyroid Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, ChinaDepartment of Breast and Thyroid Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, ChinaDepartment of Breast and Thyroid Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, ChinaDepartment of Breast and Thyroid Surgery, Guangdong General Hospital, Guangzhou, ChinaDepartment of Breast and Thyroid Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, ChinaDepartment of Breast and Thyroid Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaBackground: RP11-480I12. 5 is a newly identified long non-coding RNA (lncRNA) that has never been studied in breast cancer (BC). The biological function of RP11-480I12.5 in breast carcinoma and its underlying mechanism are still unknown.Methods: We scanned The Cancer Genome Atlas (TCGA) database and identified RP11-480I12.5 as one of the most dysregulated lncRNAs. The level of RP11-480I12.5 was assessed in BC tissue samples and BC cell lines. The prognostic value of RP11-480I12.5 expression was assessed using the Kaplan–Meier method. The biological influence of RP11-480I12.5 on BC cell lines was studied using proliferation and Transwell migration and invasion assays.Results: RP11-480I12.5 expression was upregulated in data from both the TCGA database and our own database. Moreover, Kaplan–Meier and Cox proportional hazard analyses indicated that high RP11-480I12.5 expression was related to poor overall survival. Moreover, RP11-480I12.5 promoted the proliferation, migration, and invasion of BC. RP11-480I12.5 promoted the expression of AURKA and the activation of the downstream Wnt/β-catenin pathway by sponging the microRNA (miRNA) miR-490-3p.Conclusion: Taken together, our results indicate that RP11-480I12.5 is associated with tumor progression in BCs. Our findings indicate that the lncRNA RP11-480I12.5 promotes the proliferation, migration, and invasion of BC cells through the miR-490-3p-AURKA-Wnt/β-catenin axis, which may serve as a therapeutic target in the future.https://www.frontiersin.org/article/10.3389/fonc.2020.00948/fullbreast cancerRNA RP11-480I12.5survivalproliferationmigrationinvasion |
| spellingShingle | Xinya Gao Yuanhui Lai Zhanqiang Zhang Yanfei Ma Zhizhai Luo Yanghong Li Ciqiu Yang Guanming Lu Jie Li Long Non-coding RNA RP11-480I12.5 Promotes the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-490-3p-AURKA-Wnt/β-Catenin Axis Frontiers in Oncology breast cancer RNA RP11-480I12.5 survival proliferation migration invasion |
| title | Long Non-coding RNA RP11-480I12.5 Promotes the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-490-3p-AURKA-Wnt/β-Catenin Axis |
| title_full | Long Non-coding RNA RP11-480I12.5 Promotes the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-490-3p-AURKA-Wnt/β-Catenin Axis |
| title_fullStr | Long Non-coding RNA RP11-480I12.5 Promotes the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-490-3p-AURKA-Wnt/β-Catenin Axis |
| title_full_unstemmed | Long Non-coding RNA RP11-480I12.5 Promotes the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-490-3p-AURKA-Wnt/β-Catenin Axis |
| title_short | Long Non-coding RNA RP11-480I12.5 Promotes the Proliferation, Migration, and Invasion of Breast Cancer Cells Through the miR-490-3p-AURKA-Wnt/β-Catenin Axis |
| title_sort | long non coding rna rp11 480i12 5 promotes the proliferation migration and invasion of breast cancer cells through the mir 490 3p aurka wnt β catenin axis |
| topic | breast cancer RNA RP11-480I12.5 survival proliferation migration invasion |
| url | https://www.frontiersin.org/article/10.3389/fonc.2020.00948/full |
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