Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects
We previously showed that the N6-methyladenosine (m6A) mRNA methylase is essential during Arabidopsis thaliana embryonic development. We also demonstrated that this modification is present at varying levels in all mature tissues. However, the requirement for the m6A in the mature plant was not teste...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2012-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00048/full |
| _version_ | 1819242030787723264 |
|---|---|
| author | Zsuzsanna eBodi Silin eZhong Silin eZhong Surbhi eMehra Jie eSong Jie eSong Hongying eLi Hongying eLi Neil eGraham Sean eMay Rupert George Fray |
| author_facet | Zsuzsanna eBodi Silin eZhong Silin eZhong Surbhi eMehra Jie eSong Jie eSong Hongying eLi Hongying eLi Neil eGraham Sean eMay Rupert George Fray |
| author_sort | Zsuzsanna eBodi |
| collection | DOAJ |
| description | We previously showed that the N6-methyladenosine (m6A) mRNA methylase is essential during Arabidopsis thaliana embryonic development. We also demonstrated that this modification is present at varying levels in all mature tissues. However, the requirement for the m6A in the mature plant was not tested. Here we show that a 95% reduction in m6A levels during later growth stages gives rise to plants with altered growth patterns and reduced apical dominance. The flowers of these plants commonly show defects in their floral organ number, size and identity. The global analysis of gene expression from reduced m6A plants show that a significant number of down regulated genes are involved in transport, or targeted transport, and most of the upregulated genes are involved in stress and stimulus response processes. An analysis of m6A distribution in fragmented mRNA suggests that the m6A is predominantly positioned towards the 3’ end of transcripts in a region 100-150 bp before the poly(A) tail. In addition to the analysis of the phenotypic changes in the low methylation Arabidopsis plants we will review the latest advances in the field of mRNA internal methylation |
| first_indexed | 2024-12-23T14:33:20Z |
| format | Article |
| id | doaj.art-a5ba573a11df47609b1e0fe3ffcc7558 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T14:33:20Z |
| publishDate | 2012-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a5ba573a11df47609b1e0fe3ffcc75582022-12-21T17:43:25ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2012-03-01310.3389/fpls.2012.0004820236Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defectsZsuzsanna eBodi0Silin eZhong1Silin eZhong2Surbhi eMehra3Jie eSong4Jie eSong5Hongying eLi6Hongying eLi7Neil eGraham8Sean eMay9Rupert George Fray10University of NottinghamUniversity of NottinghamCornell UniversityUniversity of NottinghamUniversity of NottinghamJohn Innes CentreUniversity of NottinghamShanxi Agricultural UniversityUniversity of NottinghamUniversity of NottinghamUniversity of NottinghamWe previously showed that the N6-methyladenosine (m6A) mRNA methylase is essential during Arabidopsis thaliana embryonic development. We also demonstrated that this modification is present at varying levels in all mature tissues. However, the requirement for the m6A in the mature plant was not tested. Here we show that a 95% reduction in m6A levels during later growth stages gives rise to plants with altered growth patterns and reduced apical dominance. The flowers of these plants commonly show defects in their floral organ number, size and identity. The global analysis of gene expression from reduced m6A plants show that a significant number of down regulated genes are involved in transport, or targeted transport, and most of the upregulated genes are involved in stress and stimulus response processes. An analysis of m6A distribution in fragmented mRNA suggests that the m6A is predominantly positioned towards the 3’ end of transcripts in a region 100-150 bp before the poly(A) tail. In addition to the analysis of the phenotypic changes in the low methylation Arabidopsis plants we will review the latest advances in the field of mRNA internal methylationhttp://journal.frontiersin.org/Journal/10.3389/fpls.2012.00048/fullpost-transcriptionalIME4METTL3mRNA methylationMT-A70 |
| spellingShingle | Zsuzsanna eBodi Silin eZhong Silin eZhong Surbhi eMehra Jie eSong Jie eSong Hongying eLi Hongying eLi Neil eGraham Sean eMay Rupert George Fray Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects Frontiers in Plant Science post-transcriptional IME4 METTL3 mRNA methylation MT-A70 |
| title | Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects |
| title_full | Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects |
| title_fullStr | Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects |
| title_full_unstemmed | Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects |
| title_short | Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects |
| title_sort | adenosine methylation in arabidopsis mrna is associated with the 3 end and reduced levels cause developmental defects |
| topic | post-transcriptional IME4 METTL3 mRNA methylation MT-A70 |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00048/full |
| work_keys_str_mv | AT zsuzsannaebodi adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT silinezhong adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT silinezhong adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT surbhiemehra adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT jieesong adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT jieesong adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT hongyingeli adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT hongyingeli adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT neilegraham adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT seanemay adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects AT rupertgeorgefray adenosinemethylationinarabidopsismrnaisassociatedwiththe3endandreducedlevelscausedevelopmentaldefects |