Insights into Arbutin Effects on Bone Cells: Towards the Development of Antioxidant Titanium Implants
Arbutin is a plant-derived glycosylated hydroquinone with antioxidant features, exploited to combat cell damage induced by oxidative stress. The latter hinders the osseointegration of bone prostheses, leading to implant failure. Little is known about arbutin antioxidant effects on human osteoblasts,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/7/579 |
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| author | Maria A. Bonifacio Giorgia Cerqueni Stefania Cometa Caterina Licini Luigia Sabbatini Monica Mattioli-Belmonte Elvira De Giglio |
| author_facet | Maria A. Bonifacio Giorgia Cerqueni Stefania Cometa Caterina Licini Luigia Sabbatini Monica Mattioli-Belmonte Elvira De Giglio |
| author_sort | Maria A. Bonifacio |
| collection | DOAJ |
| description | Arbutin is a plant-derived glycosylated hydroquinone with antioxidant features, exploited to combat cell damage induced by oxidative stress. The latter hinders the osseointegration of bone prostheses, leading to implant failure. Little is known about arbutin antioxidant effects on human osteoblasts, therefore, this study explores the in vitro protective role of arbutin on osteoblast-like cells (Saos-2) and periosteum-derived progenitor cells (PDPCs). Interestingly, cells exposed to oxidative stress were protected by arbutin, which preserved cell viability and differentiation. Starting from these encouraging results, an antioxidant coating loaded with arbutin was electrosynthesized on titanium. Therefore, for the first time, a polyacrylate-based system was designed to release the effective concentration of arbutin in situ. The innovative coating was characterized from the physico-chemical and morphological point of view to achieve an optimized system, which was in vitro tested with cells. Morpho-functional evaluations highlighted the high viability and good compatibility of the arbutin-loaded coating, which also promoted the expression of PDPC differentiation markers, even under oxidative stress. These results agreed with the coatings’ in vitro antioxidant activity, which showed a powerful scavenging effect against DPPH radicals. Taken together, the obtained results open intriguing opportunities for the further development of natural bioactive coatings for orthopedic titanium implants. |
| first_indexed | 2024-03-10T18:43:47Z |
| format | Article |
| id | doaj.art-a5baa658afb54c2384a0950ad0dfe437 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T18:43:47Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a5baa658afb54c2384a0950ad0dfe4372023-11-20T05:40:49ZengMDPI AGAntioxidants2076-39212020-07-019757910.3390/antiox9070579Insights into Arbutin Effects on Bone Cells: Towards the Development of Antioxidant Titanium ImplantsMaria A. Bonifacio0Giorgia Cerqueni1Stefania Cometa2Caterina Licini3Luigia Sabbatini4Monica Mattioli-Belmonte5Elvira De Giglio6Department of Chemistry, University of Bari Aldo Moro, 70126 Bari, ItalyDISCLIMO Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, 60126 Ancona, ItalyJaber Innovation s.r.l., 00144 Roma, ItalyDISCLIMO Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, 60126 Ancona, ItalyDepartment of Chemistry, University of Bari Aldo Moro, 70126 Bari, ItalyDISCLIMO Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, 60126 Ancona, ItalyDepartment of Chemistry, University of Bari Aldo Moro, 70126 Bari, ItalyArbutin is a plant-derived glycosylated hydroquinone with antioxidant features, exploited to combat cell damage induced by oxidative stress. The latter hinders the osseointegration of bone prostheses, leading to implant failure. Little is known about arbutin antioxidant effects on human osteoblasts, therefore, this study explores the in vitro protective role of arbutin on osteoblast-like cells (Saos-2) and periosteum-derived progenitor cells (PDPCs). Interestingly, cells exposed to oxidative stress were protected by arbutin, which preserved cell viability and differentiation. Starting from these encouraging results, an antioxidant coating loaded with arbutin was electrosynthesized on titanium. Therefore, for the first time, a polyacrylate-based system was designed to release the effective concentration of arbutin in situ. The innovative coating was characterized from the physico-chemical and morphological point of view to achieve an optimized system, which was in vitro tested with cells. Morpho-functional evaluations highlighted the high viability and good compatibility of the arbutin-loaded coating, which also promoted the expression of PDPC differentiation markers, even under oxidative stress. These results agreed with the coatings’ in vitro antioxidant activity, which showed a powerful scavenging effect against DPPH radicals. Taken together, the obtained results open intriguing opportunities for the further development of natural bioactive coatings for orthopedic titanium implants.https://www.mdpi.com/2076-3921/9/7/579arbutinoxidative stresscoatingsurface characterizationtitanium implantbone cells |
| spellingShingle | Maria A. Bonifacio Giorgia Cerqueni Stefania Cometa Caterina Licini Luigia Sabbatini Monica Mattioli-Belmonte Elvira De Giglio Insights into Arbutin Effects on Bone Cells: Towards the Development of Antioxidant Titanium Implants Antioxidants arbutin oxidative stress coating surface characterization titanium implant bone cells |
| title | Insights into Arbutin Effects on Bone Cells: Towards the Development of Antioxidant Titanium Implants |
| title_full | Insights into Arbutin Effects on Bone Cells: Towards the Development of Antioxidant Titanium Implants |
| title_fullStr | Insights into Arbutin Effects on Bone Cells: Towards the Development of Antioxidant Titanium Implants |
| title_full_unstemmed | Insights into Arbutin Effects on Bone Cells: Towards the Development of Antioxidant Titanium Implants |
| title_short | Insights into Arbutin Effects on Bone Cells: Towards the Development of Antioxidant Titanium Implants |
| title_sort | insights into arbutin effects on bone cells towards the development of antioxidant titanium implants |
| topic | arbutin oxidative stress coating surface characterization titanium implant bone cells |
| url | https://www.mdpi.com/2076-3921/9/7/579 |
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