<i>Glycyrrhiza uralensis</i> Nodules: Histological and Ultrastructural Organization and Tubulin Cytoskeleton Dynamics
Chinese liquorice (<i>Glycyrrhiza uralensis</i> Fisch. ex DC.) is widely used in the food industry and as a medicine. Like other legumes, <i>G. uralensis</i> forms symbiotic nodules. However, the structural organization of <i>G. uralensis</i> nodules is poorly und...
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MDPI AG
2021-12-01
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| Series: | Agronomy |
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| author | Anna V. Tsyganova Anna B. Kitaeva Artemii P. Gorshkov Pyotr G. Kusakin Alexandra R. Sadovskaya Yaroslav G. Borisov Viktor E. Tsyganov |
| author_facet | Anna V. Tsyganova Anna B. Kitaeva Artemii P. Gorshkov Pyotr G. Kusakin Alexandra R. Sadovskaya Yaroslav G. Borisov Viktor E. Tsyganov |
| author_sort | Anna V. Tsyganova |
| collection | DOAJ |
| description | Chinese liquorice (<i>Glycyrrhiza uralensis</i> Fisch. ex DC.) is widely used in the food industry and as a medicine. Like other legumes, <i>G. uralensis</i> forms symbiotic nodules. However, the structural organization of <i>G. uralensis</i> nodules is poorly understood. In this study, we analyzed the histological and ultrastructural organization and dynamics of the tubulin cytoskeleton in various cells from different histological zones of indeterminate nodules formed by two strains of <i>Mesorhizobium</i> sp. The unusual walls of infection threads and formation of multiple symbiosomes with several swollen bacteroids were observed. A large amount of poly-β-hydroxybutyrate accumulated in the bacteroids, while the vacuoles of meristematic and uninfected cells contained drop-shaped osmiophilic inclusions. Immunolocalization of the tubulin cytoskeleton and quantitative analysis of cytoskeletal elements revealed patterns of cortical microtubules in meristematic, infected and uninfected cells, and of endoplasmic microtubules associated with infection structures, typical of indeterminate nodules. The intermediate pattern of endoplasmic microtubules in infected cells was correlated with disordered arrangement of symbiosomes. Thus, analysis of the structural organization of <i>G. uralensis</i> nodules revealed some ancestral features more characteristic of determinate nodules, demonstrating the evolutionary closeness of <i>G. uralensis</i> nodulation to more ancient members of the legume family. |
| first_indexed | 2024-03-10T04:40:46Z |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T04:40:46Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-a5be167642aa46138020c93da370d5e22023-11-23T03:22:58ZengMDPI AGAgronomy2073-43952021-12-011112250810.3390/agronomy11122508<i>Glycyrrhiza uralensis</i> Nodules: Histological and Ultrastructural Organization and Tubulin Cytoskeleton DynamicsAnna V. Tsyganova0Anna B. Kitaeva1Artemii P. Gorshkov2Pyotr G. Kusakin3Alexandra R. Sadovskaya4Yaroslav G. Borisov5Viktor E. Tsyganov6Laboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, 196608 Saint Petersburg, RussiaLaboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, 196608 Saint Petersburg, RussiaLaboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, 196608 Saint Petersburg, RussiaLaboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, 196608 Saint Petersburg, RussiaFaculty of Biology, Saint Petersburg State University, 199034 Saint Petersburg, RussiaFaculty of Biology, Saint Petersburg State University, 199034 Saint Petersburg, RussiaLaboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, 196608 Saint Petersburg, RussiaChinese liquorice (<i>Glycyrrhiza uralensis</i> Fisch. ex DC.) is widely used in the food industry and as a medicine. Like other legumes, <i>G. uralensis</i> forms symbiotic nodules. However, the structural organization of <i>G. uralensis</i> nodules is poorly understood. In this study, we analyzed the histological and ultrastructural organization and dynamics of the tubulin cytoskeleton in various cells from different histological zones of indeterminate nodules formed by two strains of <i>Mesorhizobium</i> sp. The unusual walls of infection threads and formation of multiple symbiosomes with several swollen bacteroids were observed. A large amount of poly-β-hydroxybutyrate accumulated in the bacteroids, while the vacuoles of meristematic and uninfected cells contained drop-shaped osmiophilic inclusions. Immunolocalization of the tubulin cytoskeleton and quantitative analysis of cytoskeletal elements revealed patterns of cortical microtubules in meristematic, infected and uninfected cells, and of endoplasmic microtubules associated with infection structures, typical of indeterminate nodules. The intermediate pattern of endoplasmic microtubules in infected cells was correlated with disordered arrangement of symbiosomes. Thus, analysis of the structural organization of <i>G. uralensis</i> nodules revealed some ancestral features more characteristic of determinate nodules, demonstrating the evolutionary closeness of <i>G. uralensis</i> nodulation to more ancient members of the legume family.https://www.mdpi.com/2073-4395/11/12/2508legume-rhizobial symbiosisindeterminate nodulesinfection threadbacteroidsymbiosomesmicrotubules |
| spellingShingle | Anna V. Tsyganova Anna B. Kitaeva Artemii P. Gorshkov Pyotr G. Kusakin Alexandra R. Sadovskaya Yaroslav G. Borisov Viktor E. Tsyganov <i>Glycyrrhiza uralensis</i> Nodules: Histological and Ultrastructural Organization and Tubulin Cytoskeleton Dynamics Agronomy legume-rhizobial symbiosis indeterminate nodules infection thread bacteroid symbiosomes microtubules |
| title | <i>Glycyrrhiza uralensis</i> Nodules: Histological and Ultrastructural Organization and Tubulin Cytoskeleton Dynamics |
| title_full | <i>Glycyrrhiza uralensis</i> Nodules: Histological and Ultrastructural Organization and Tubulin Cytoskeleton Dynamics |
| title_fullStr | <i>Glycyrrhiza uralensis</i> Nodules: Histological and Ultrastructural Organization and Tubulin Cytoskeleton Dynamics |
| title_full_unstemmed | <i>Glycyrrhiza uralensis</i> Nodules: Histological and Ultrastructural Organization and Tubulin Cytoskeleton Dynamics |
| title_short | <i>Glycyrrhiza uralensis</i> Nodules: Histological and Ultrastructural Organization and Tubulin Cytoskeleton Dynamics |
| title_sort | i glycyrrhiza uralensis i nodules histological and ultrastructural organization and tubulin cytoskeleton dynamics |
| topic | legume-rhizobial symbiosis indeterminate nodules infection thread bacteroid symbiosomes microtubules |
| url | https://www.mdpi.com/2073-4395/11/12/2508 |
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