Transcriptomic and physiological responses of contrasting maize genotypes to drought stress
Drought is a significant environmental stress factor that adversely affects maize productivity. However, many details regarding the molecular mechanisms of maize against drought are still unclear. In this study, leaf transcriptomics and physiological traits of two maize genotypes with differing drou...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.928897/full |
| _version_ | 1811343159737712640 |
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| author | Yifan Wang Haoxue Guo Xi Wu Jiarui Wang Hongjie Li Renhe Zhang |
| author_facet | Yifan Wang Haoxue Guo Xi Wu Jiarui Wang Hongjie Li Renhe Zhang |
| author_sort | Yifan Wang |
| collection | DOAJ |
| description | Drought is a significant environmental stress factor that adversely affects maize productivity. However, many details regarding the molecular mechanisms of maize against drought are still unclear. In this study, leaf transcriptomics and physiological traits of two maize genotypes with differing drought resistance were analyzed. Transcriptome sequencing identified 8985 and 7305 differentially expressed genes (DEGs) in SD902 and SD609, respectively. Functional analysis suggested that numerous genes are highly involved in oxidative defense, protein modification, photosynthesis, phytohormone response, MAPK signaling, and transcription factors (TFs). Compared to SD902, SD609 had a higher expression of DEGs related to antioxidant enzymes, photosynthetic electron transport, heat shock proteins, and indole-3-acetic acid (IAA) signaling under drought conditions, which might contribute to its tolerance mechanisms to drought. Stress-induced TFs may play a crucial regulatory role in genotypic differences. Moreover, the physiological changes and gene expression abundance determined using quantitative reverse transcription polymerase chain reaction were consistent with the RNA sequencing data. The study results suggest that the higher drought tolerance of SD609 than SD902 can be attributed to stronger stress defense capabilities, IAA signal transduction, and more stable photosynthesis. Our findings provide new insights into the molecular mechanisms of maize against drought stress, and the candidate genes identified may be used in breeding drought-tolerant maize cultivars. |
| first_indexed | 2024-04-13T19:24:47Z |
| format | Article |
| id | doaj.art-a5be2c074e01483595458089b854af94 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T19:24:47Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a5be2c074e01483595458089b854af942022-12-22T02:33:25ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-08-011310.3389/fpls.2022.928897928897Transcriptomic and physiological responses of contrasting maize genotypes to drought stressYifan WangHaoxue GuoXi WuJiarui WangHongjie LiRenhe ZhangDrought is a significant environmental stress factor that adversely affects maize productivity. However, many details regarding the molecular mechanisms of maize against drought are still unclear. In this study, leaf transcriptomics and physiological traits of two maize genotypes with differing drought resistance were analyzed. Transcriptome sequencing identified 8985 and 7305 differentially expressed genes (DEGs) in SD902 and SD609, respectively. Functional analysis suggested that numerous genes are highly involved in oxidative defense, protein modification, photosynthesis, phytohormone response, MAPK signaling, and transcription factors (TFs). Compared to SD902, SD609 had a higher expression of DEGs related to antioxidant enzymes, photosynthetic electron transport, heat shock proteins, and indole-3-acetic acid (IAA) signaling under drought conditions, which might contribute to its tolerance mechanisms to drought. Stress-induced TFs may play a crucial regulatory role in genotypic differences. Moreover, the physiological changes and gene expression abundance determined using quantitative reverse transcription polymerase chain reaction were consistent with the RNA sequencing data. The study results suggest that the higher drought tolerance of SD609 than SD902 can be attributed to stronger stress defense capabilities, IAA signal transduction, and more stable photosynthesis. Our findings provide new insights into the molecular mechanisms of maize against drought stress, and the candidate genes identified may be used in breeding drought-tolerant maize cultivars.https://www.frontiersin.org/articles/10.3389/fpls.2022.928897/fullmaizedrought stresstranscriptome sequencingphysiological analysistolerance mechanism |
| spellingShingle | Yifan Wang Haoxue Guo Xi Wu Jiarui Wang Hongjie Li Renhe Zhang Transcriptomic and physiological responses of contrasting maize genotypes to drought stress Frontiers in Plant Science maize drought stress transcriptome sequencing physiological analysis tolerance mechanism |
| title | Transcriptomic and physiological responses of contrasting maize genotypes to drought stress |
| title_full | Transcriptomic and physiological responses of contrasting maize genotypes to drought stress |
| title_fullStr | Transcriptomic and physiological responses of contrasting maize genotypes to drought stress |
| title_full_unstemmed | Transcriptomic and physiological responses of contrasting maize genotypes to drought stress |
| title_short | Transcriptomic and physiological responses of contrasting maize genotypes to drought stress |
| title_sort | transcriptomic and physiological responses of contrasting maize genotypes to drought stress |
| topic | maize drought stress transcriptome sequencing physiological analysis tolerance mechanism |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.928897/full |
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