Molecular Diagnostic Methods for the Detection of Leptospirosis
Leptospirosis is a widespread infectious disease caused by the spirochete Leptospira. The clinical features of leptospirosis are fever, headache, vomiting, jaundice, and the acute form of the disease is commonly called Weil’s disease. The microscopic agglutination test (MAT) is a gold standard metho...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2022-06-01
|
| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/molecular-diagnostic-methods-for-the-detection-of-leptospirosis/ |
| _version_ | 1828332011891195904 |
|---|---|
| author | Rethinavelu Gayathri Vishwakarma Archana Mohandass Ramya |
| author_facet | Rethinavelu Gayathri Vishwakarma Archana Mohandass Ramya |
| author_sort | Rethinavelu Gayathri |
| collection | DOAJ |
| description | Leptospirosis is a widespread infectious disease caused by the spirochete Leptospira. The clinical features of leptospirosis are fever, headache, vomiting, jaundice, and the acute form of the disease is commonly called Weil’s disease. The microscopic agglutination test (MAT) is a gold standard method used to detect leptospirosis. However, it requires 14 days of time and skilled personnel to detect leptospirosis. Various molecular methods were developed for the rapid detection process, including polymerase chain reaction (PCR), multiplex PCR, nested PCR, real-time PCR, and Loop-mediated isothermal amplification (LAMP). Other immuno-based biosensor kits are readily available for the diagnosis of leptospirosis. Though these methods claim to be highly sensitive and specific, each method has its drawbacks. This review discusses the different molecular diagnostic techniques applied for the diagnosis of leptospirosis; elaborating on each method’s sensitivity, specificity, and detection time and the different samples of water, blood, and urine used. |
| first_indexed | 2024-04-13T21:00:11Z |
| format | Article |
| id | doaj.art-a5c1b48e3c57418e8e5b7cb16248ca6f |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-13T21:00:11Z |
| publishDate | 2022-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-a5c1b48e3c57418e8e5b7cb16248ca6f2022-12-22T02:30:10ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2022-06-01162782795https://doi.org/10.22207/JPAM.16.2.24Molecular Diagnostic Methods for the Detection of LeptospirosisRethinavelu Gayathrihttps://orcid.org/0000-0001-9200-1630Vishwakarma Archanahttps://orcid.org/0000-0002-4820-6468Mohandass Ramyahttps://orcid.org/0000-0003-4448-920XLeptospirosis is a widespread infectious disease caused by the spirochete Leptospira. The clinical features of leptospirosis are fever, headache, vomiting, jaundice, and the acute form of the disease is commonly called Weil’s disease. The microscopic agglutination test (MAT) is a gold standard method used to detect leptospirosis. However, it requires 14 days of time and skilled personnel to detect leptospirosis. Various molecular methods were developed for the rapid detection process, including polymerase chain reaction (PCR), multiplex PCR, nested PCR, real-time PCR, and Loop-mediated isothermal amplification (LAMP). Other immuno-based biosensor kits are readily available for the diagnosis of leptospirosis. Though these methods claim to be highly sensitive and specific, each method has its drawbacks. This review discusses the different molecular diagnostic techniques applied for the diagnosis of leptospirosis; elaborating on each method’s sensitivity, specificity, and detection time and the different samples of water, blood, and urine used.https://microbiologyjournal.org/molecular-diagnostic-methods-for-the-detection-of-leptospirosis/leptospirosismatlamppcrmarker genes |
| spellingShingle | Rethinavelu Gayathri Vishwakarma Archana Mohandass Ramya Molecular Diagnostic Methods for the Detection of Leptospirosis Journal of Pure and Applied Microbiology leptospirosis mat lamp pcr marker genes |
| title | Molecular Diagnostic Methods for the Detection of Leptospirosis |
| title_full | Molecular Diagnostic Methods for the Detection of Leptospirosis |
| title_fullStr | Molecular Diagnostic Methods for the Detection of Leptospirosis |
| title_full_unstemmed | Molecular Diagnostic Methods for the Detection of Leptospirosis |
| title_short | Molecular Diagnostic Methods for the Detection of Leptospirosis |
| title_sort | molecular diagnostic methods for the detection of leptospirosis |
| topic | leptospirosis mat lamp pcr marker genes |
| url | https://microbiologyjournal.org/molecular-diagnostic-methods-for-the-detection-of-leptospirosis/ |
| work_keys_str_mv | AT rethinavelugayathri moleculardiagnosticmethodsforthedetectionofleptospirosis AT vishwakarmaarchana moleculardiagnosticmethodsforthedetectionofleptospirosis AT mohandassramya moleculardiagnosticmethodsforthedetectionofleptospirosis |