Application study of curcumin fluorescent complex coated with pharmaceutical excipients for cell imaging
Taking curcumin as the starting point, β-cyclodextrin was introduced on both sides, and lipid-soluble curcumin was coated by acrylic resin using oil-in-water strategy. Four different types of curcumin fluorescent complexes EPO-Curcumin (EPO-Cur), L100-55-Curcumin (L100-55-Cur), EPO -Curcumin-β-cyclo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Chemistry |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1153729/full |
| _version_ | 1797869639781318656 |
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| author | Chen Shao Xiaoli Zhang Shihe Shao Shihe Shao Feng Jin |
| author_facet | Chen Shao Xiaoli Zhang Shihe Shao Shihe Shao Feng Jin |
| author_sort | Chen Shao |
| collection | DOAJ |
| description | Taking curcumin as the starting point, β-cyclodextrin was introduced on both sides, and lipid-soluble curcumin was coated by acrylic resin using oil-in-water strategy. Four different types of curcumin fluorescent complexes EPO-Curcumin (EPO-Cur), L100-55-Curcumin (L100-55-Cur), EPO -Curcumin-β-cyclodextrin (EPO-Cur-β-cd) and L100-55-Curcumin-β-cyclodextrin (L100-55-Cur-β-cd) were prepared to solve their own solubility and biocompatibility issues. The prepared curcumin fluorescent complexes were characterized and tested by spectroscopy. The characteristic peaks of 3446 cm−1 (hydroxyl group), 1735cm−1(carbonyl group) and 1455 cm−1 (aromatic group) were determined in the infrared spectrum. In the fluorescence emission spectrum, it was found that the emission intensity of different curcumin fluorescent complexes in polar solvents reached hundreds of times. Through the transmission electron microscopy shows that acrylic resin tightly coats curcumin into rods or clusters. In order to observe their compatibility with tumor cells more directly, live cell fluorescence imaging was carried out, and it was found that all four kinds of curcumin fluorescence complexes had good biocompatibility. In particular, the effect of EPO-Cur-β-cd and L100-55-Cur-β-cd is better than that of EPO-Cur and L100-55-Cur. |
| first_indexed | 2024-04-10T00:14:42Z |
| format | Article |
| id | doaj.art-a5c33f9a72b044d3948d7ec1f68b7fcc |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-10T00:14:42Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-a5c33f9a72b044d3948d7ec1f68b7fcc2023-03-16T06:03:55ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-03-011110.3389/fchem.2023.11537291153729Application study of curcumin fluorescent complex coated with pharmaceutical excipients for cell imagingChen Shao0Xiaoli Zhang1Shihe Shao2Shihe Shao3Feng Jin4Affiliated Hospital of Jiangsu University, Zhenjiang, ChinaDepartment of Critical Care Medicine, Affiliated Yixing People’s Hospital, Jiangsu University, Yixing, ChinaAffiliated Hospital of Jiangsu University, Zhenjiang, ChinaDepartment of Critical Care Medicine, Affiliated Yixing People’s Hospital, Jiangsu University, Yixing, ChinaMedical College of Anhui University of Science and Technology, Huainan, ChinaTaking curcumin as the starting point, β-cyclodextrin was introduced on both sides, and lipid-soluble curcumin was coated by acrylic resin using oil-in-water strategy. Four different types of curcumin fluorescent complexes EPO-Curcumin (EPO-Cur), L100-55-Curcumin (L100-55-Cur), EPO -Curcumin-β-cyclodextrin (EPO-Cur-β-cd) and L100-55-Curcumin-β-cyclodextrin (L100-55-Cur-β-cd) were prepared to solve their own solubility and biocompatibility issues. The prepared curcumin fluorescent complexes were characterized and tested by spectroscopy. The characteristic peaks of 3446 cm−1 (hydroxyl group), 1735cm−1(carbonyl group) and 1455 cm−1 (aromatic group) were determined in the infrared spectrum. In the fluorescence emission spectrum, it was found that the emission intensity of different curcumin fluorescent complexes in polar solvents reached hundreds of times. Through the transmission electron microscopy shows that acrylic resin tightly coats curcumin into rods or clusters. In order to observe their compatibility with tumor cells more directly, live cell fluorescence imaging was carried out, and it was found that all four kinds of curcumin fluorescence complexes had good biocompatibility. In particular, the effect of EPO-Cur-β-cd and L100-55-Cur-β-cd is better than that of EPO-Cur and L100-55-Cur.https://www.frontiersin.org/articles/10.3389/fchem.2023.1153729/fullcurcuminβ-cyclodextrinacrylic resinpharmaceutical excipientscell imaging |
| spellingShingle | Chen Shao Xiaoli Zhang Shihe Shao Shihe Shao Feng Jin Application study of curcumin fluorescent complex coated with pharmaceutical excipients for cell imaging Frontiers in Chemistry curcumin β-cyclodextrin acrylic resin pharmaceutical excipients cell imaging |
| title | Application study of curcumin fluorescent complex coated with pharmaceutical excipients for cell imaging |
| title_full | Application study of curcumin fluorescent complex coated with pharmaceutical excipients for cell imaging |
| title_fullStr | Application study of curcumin fluorescent complex coated with pharmaceutical excipients for cell imaging |
| title_full_unstemmed | Application study of curcumin fluorescent complex coated with pharmaceutical excipients for cell imaging |
| title_short | Application study of curcumin fluorescent complex coated with pharmaceutical excipients for cell imaging |
| title_sort | application study of curcumin fluorescent complex coated with pharmaceutical excipients for cell imaging |
| topic | curcumin β-cyclodextrin acrylic resin pharmaceutical excipients cell imaging |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1153729/full |
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