Safety Assessment of <i>Bacillus subtilis</i> G8 Isolated from Natto for Food Application
Various bacteria are widely used as food-fermenting agents, including Lactobacillus, Bifidobacterium, and Bacillus. Despite they are generally recognized as safe to be consumed by humans, those bacteria could potentially cause antibiotic resistance as they could acquire and transfer antibiotic resis...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2024-02-01
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| Series: | Journal of Tropical Biodiversity and Biotechnology |
| Subjects: | |
| Online Access: | https://journal.ugm.ac.id/jtbb/article/view/87079 |
| _version_ | 1797254059530387456 |
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| author | Nathania Calista Putri Hans Victor Vivian Litanto Reinhard Pinontoan Juandy Jo |
| author_facet | Nathania Calista Putri Hans Victor Vivian Litanto Reinhard Pinontoan Juandy Jo |
| author_sort | Nathania Calista Putri |
| collection | DOAJ |
| description | Various bacteria are widely used as food-fermenting agents, including Lactobacillus, Bifidobacterium, and Bacillus. Despite they are generally recognized as safe to be consumed by humans, those bacteria could potentially cause antibiotic resistance as they could acquire and transfer antibiotic resistance genes from or to other microbes within the human gastrointestinal tract. Profiling antibiotic resistance pattern in those bacteria is therefore important to control the spread of antibiotic resistance. In this study, antibiotic resistance profile of Bacillus subtilis G8 was assessed. B. subtilis G8 had been isolated from commercialised Japanese natto in Indonesia and had been previously reported for its fibrinolytic characteristics. The antibiotic resistance phenotype and genotype of B. subtilis G8 were assessed through the Kirby-Bauer disk diffusion method and whole-genome analysis, respectively. B. subtilis G8 exhibited resistance towards Oxacillin, Lincomycin and Tiamulin-Lefamulin. The bioinformatics analysis indicated several responsible genes mediating those resistance, i.e., ybxI (for Oxacillin), lmrB (for Lincomycin) and vmlR (for Lincomycin and Tiamulin-Lefamulin). All identified genes were found in the chromosomal DNA. Further analysis found no mobile genetic elements within the genome, therefore reducing a risk of resistance gene transfer via plasmid and subsequently supporting safety profile of B. subtilis G8 in food fermentation usage. |
| first_indexed | 2024-04-24T21:43:56Z |
| format | Article |
| id | doaj.art-a5c54a2f62ac4027af67c2b7947df422 |
| institution | Directory Open Access Journal |
| issn | 2540-9573 2540-9581 |
| language | English |
| last_indexed | 2024-04-24T21:43:56Z |
| publishDate | 2024-02-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Journal of Tropical Biodiversity and Biotechnology |
| spelling | doaj.art-a5c54a2f62ac4027af67c2b7947df4222024-03-21T03:30:47ZengUniversitas Gadjah MadaJournal of Tropical Biodiversity and Biotechnology2540-95732540-95812024-02-019110.22146/jtbb.8707935303Safety Assessment of <i>Bacillus subtilis</i> G8 Isolated from Natto for Food ApplicationNathania Calista Putri0Hans Victor1Vivian Litanto2Reinhard Pinontoan3Juandy Jo4Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Tangerang 15811, Banten, Indonesia.Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Tangerang 15811, Banten, Indonesia.Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Tangerang 15811, Banten, Indonesia.Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Tangerang 15811, Banten, Indonesia.Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Tangerang 15811, Banten, Indonesia; Mochtar Riady Institute for Nanotechnology, Jl. Boulevard Jendral Sudirman No.1688, Tangerang 15811, Banten, Indonesia.Various bacteria are widely used as food-fermenting agents, including Lactobacillus, Bifidobacterium, and Bacillus. Despite they are generally recognized as safe to be consumed by humans, those bacteria could potentially cause antibiotic resistance as they could acquire and transfer antibiotic resistance genes from or to other microbes within the human gastrointestinal tract. Profiling antibiotic resistance pattern in those bacteria is therefore important to control the spread of antibiotic resistance. In this study, antibiotic resistance profile of Bacillus subtilis G8 was assessed. B. subtilis G8 had been isolated from commercialised Japanese natto in Indonesia and had been previously reported for its fibrinolytic characteristics. The antibiotic resistance phenotype and genotype of B. subtilis G8 were assessed through the Kirby-Bauer disk diffusion method and whole-genome analysis, respectively. B. subtilis G8 exhibited resistance towards Oxacillin, Lincomycin and Tiamulin-Lefamulin. The bioinformatics analysis indicated several responsible genes mediating those resistance, i.e., ybxI (for Oxacillin), lmrB (for Lincomycin) and vmlR (for Lincomycin and Tiamulin-Lefamulin). All identified genes were found in the chromosomal DNA. Further analysis found no mobile genetic elements within the genome, therefore reducing a risk of resistance gene transfer via plasmid and subsequently supporting safety profile of B. subtilis G8 in food fermentation usage.https://journal.ugm.ac.id/jtbb/article/view/87079bacillus subtilis g8nattoantibiotic resistancedisk diffusion methodwhole-genome sequencing |
| spellingShingle | Nathania Calista Putri Hans Victor Vivian Litanto Reinhard Pinontoan Juandy Jo Safety Assessment of <i>Bacillus subtilis</i> G8 Isolated from Natto for Food Application Journal of Tropical Biodiversity and Biotechnology bacillus subtilis g8 natto antibiotic resistance disk diffusion method whole-genome sequencing |
| title | Safety Assessment of <i>Bacillus subtilis</i> G8 Isolated from Natto for Food Application |
| title_full | Safety Assessment of <i>Bacillus subtilis</i> G8 Isolated from Natto for Food Application |
| title_fullStr | Safety Assessment of <i>Bacillus subtilis</i> G8 Isolated from Natto for Food Application |
| title_full_unstemmed | Safety Assessment of <i>Bacillus subtilis</i> G8 Isolated from Natto for Food Application |
| title_short | Safety Assessment of <i>Bacillus subtilis</i> G8 Isolated from Natto for Food Application |
| title_sort | safety assessment of i bacillus subtilis i g8 isolated from natto for food application |
| topic | bacillus subtilis g8 natto antibiotic resistance disk diffusion method whole-genome sequencing |
| url | https://journal.ugm.ac.id/jtbb/article/view/87079 |
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