Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus Platform
Lujo virus (LUJV), a highly pathogenic arenavirus, was first identified in 2008 in Zambia. To aid the identification of effective therapeutics for LUJV, we developed a recombinant reporter virus system, confirming reporter LUJV comparability with wild-type virus and its utility in high-throughput an...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-06-01
|
Series: | Viruses |
Subjects: | |
Online Access: | https://www.mdpi.com/1999-4915/13/7/1255 |
_version_ | 1797528544641810432 |
---|---|
author | Stephen R. Welch Jessica R. Spengler Sarah C. Genzer Payel Chatterjee Mike Flint Éric Bergeron Joel M. Montgomery Stuart T. Nichol César G. Albariño Christina F. Spiropoulou |
author_facet | Stephen R. Welch Jessica R. Spengler Sarah C. Genzer Payel Chatterjee Mike Flint Éric Bergeron Joel M. Montgomery Stuart T. Nichol César G. Albariño Christina F. Spiropoulou |
author_sort | Stephen R. Welch |
collection | DOAJ |
description | Lujo virus (LUJV), a highly pathogenic arenavirus, was first identified in 2008 in Zambia. To aid the identification of effective therapeutics for LUJV, we developed a recombinant reporter virus system, confirming reporter LUJV comparability with wild-type virus and its utility in high-throughput antiviral screening assays. Using this system, we evaluated compounds with known and unknown efficacy against related arenaviruses, with the aim of identifying LUJV-specific and potential new pan-arenavirus antivirals. We identified six compounds demonstrating robust anti-LUJV activity, including several compounds with previously reported activity against other arenaviruses. These data provide critical evidence for developing broad-spectrum antivirals against high-consequence arenaviruses. |
first_indexed | 2024-03-10T10:00:54Z |
format | Article |
id | doaj.art-a5c5b74b8d64468b9220e208da5b46b7 |
institution | Directory Open Access Journal |
issn | 1999-4915 |
language | English |
last_indexed | 2024-03-10T10:00:54Z |
publishDate | 2021-06-01 |
publisher | MDPI AG |
record_format | Article |
series | Viruses |
spelling | doaj.art-a5c5b74b8d64468b9220e208da5b46b72023-11-22T01:58:41ZengMDPI AGViruses1999-49152021-06-01137125510.3390/v13071255Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus PlatformStephen R. Welch0Jessica R. Spengler1Sarah C. Genzer2Payel Chatterjee3Mike Flint4Éric Bergeron5Joel M. Montgomery6Stuart T. Nichol7César G. Albariño8Christina F. Spiropoulou9Viral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USAViral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USAViral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USAViral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USAViral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USAViral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USAViral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USAViral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USAViral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USAViral Special Pathogens Branch, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30329, USALujo virus (LUJV), a highly pathogenic arenavirus, was first identified in 2008 in Zambia. To aid the identification of effective therapeutics for LUJV, we developed a recombinant reporter virus system, confirming reporter LUJV comparability with wild-type virus and its utility in high-throughput antiviral screening assays. Using this system, we evaluated compounds with known and unknown efficacy against related arenaviruses, with the aim of identifying LUJV-specific and potential new pan-arenavirus antivirals. We identified six compounds demonstrating robust anti-LUJV activity, including several compounds with previously reported activity against other arenaviruses. These data provide critical evidence for developing broad-spectrum antivirals against high-consequence arenaviruses.https://www.mdpi.com/1999-4915/13/7/1255Lujo virusviral hemorrhagic feverarenavirusreporter virusreverse geneticsantiviral screen |
spellingShingle | Stephen R. Welch Jessica R. Spengler Sarah C. Genzer Payel Chatterjee Mike Flint Éric Bergeron Joel M. Montgomery Stuart T. Nichol César G. Albariño Christina F. Spiropoulou Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus Platform Viruses Lujo virus viral hemorrhagic fever arenavirus reporter virus reverse genetics antiviral screen |
title | Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus Platform |
title_full | Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus Platform |
title_fullStr | Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus Platform |
title_full_unstemmed | Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus Platform |
title_short | Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus Platform |
title_sort | screening and identification of lujo virus inhibitors using a recombinant reporter virus platform |
topic | Lujo virus viral hemorrhagic fever arenavirus reporter virus reverse genetics antiviral screen |
url | https://www.mdpi.com/1999-4915/13/7/1255 |
work_keys_str_mv | AT stephenrwelch screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform AT jessicarspengler screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform AT sarahcgenzer screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform AT payelchatterjee screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform AT mikeflint screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform AT ericbergeron screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform AT joelmmontgomery screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform AT stuarttnichol screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform AT cesargalbarino screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform AT christinafspiropoulou screeningandidentificationoflujovirusinhibitorsusingarecombinantreportervirusplatform |